MLS1 malate synthase MLS1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MLS1
Official Full Name: malate synthase MLS1
Primary source: SGD:S000005061
Locus tag: YNL117W
See related: AllianceGenome:SGD:S000005061; FungiDB:YNL117W; VEuPathDB:YNL117W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables malate synthase activity. Involved in glyoxylate cycle. Located in cytosol and peroxisomal matrix. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MLS1 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (406358..408022)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Using MLS1 promoters from eight yeast species, we find that large differences in gene expression levels do not generate detectable fitness effects. However, we also find a large drop in fitness below a certain low level of expression, implying that the S. cerevisiae MLS1 promoter resides on a fitness plateau.
Title: Divergent MLS1 Promoters Lie on a Fitness Plateau for Gene Expression.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables malate synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables malate synthase activity	IEA Inferred from Electronic Annotation more info
enables malate synthase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in glyoxylate cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in glyoxylate cycle	IEA Inferred from Electronic Annotation more info
involved_in glyoxylate cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tricarboxylic acid cycle	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	HDA more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in peroxisomal matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in peroxisomal matrix	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisomal matrix	IEA Inferred from Electronic Annotation more info
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisome	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: malate synthase MLS1

NP_014282.1

EC 2.3.3.9

Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic; can accept butyryl-CoA as acyl-CoA donor in addition to traditional substrate acetyl-CoA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

406358..408022

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182955.1 → NP_014282.1 TPA: malate synthase MLS1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014282.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZRW6, B0KZR8, B0KZS7, B0KZU5, B0KZY1, B0L026, D6W165, P30952, Q4KQ64, Q4KQ91, Q4KQC3, Q4KQC4, Q4KQC8

UniProtKB/TrEMBL

G2WLY9, N1NXW0

Conserved Domains (1) summary

cd00727
Location:31 → 542

malate_synt_A; Malate synthase A (MSA), present in some bacteria, plants and fungi. Prokaryotic MSAs tend to be monomeric, whereas eukaryotic enzymes are homomultimers. In general, malate synthase catalyzes the Claisen condensation of glyoxylate and acetyl-CoA to ...