DCP2 decapping enzyme complex catalytic subunit [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DCP2
Official Full Name: decapping enzyme complex catalytic subunit
Primary source: SGD:S000005062
Locus tag: YNL118C
See related: AllianceGenome:SGD:S000005062; FungiDB:YNL118C; VEuPathDB:YNL118C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PSU1
Summary: Enables chromatin binding activity; hydrolase activity; and mRNA binding activity. Involved in mRNA methylguanosine-cap decapping; positive regulation of transcription initiation by RNA polymerase II; and stress granule assembly. Located in P-body; cytoplasmic side of membrane; and nucleus. Part of RNA decapping complex. Orthologous to human DCP2 (decapping mRNA 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See DCP2 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (402652..405564, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dcp2 C-terminal cis-binding elements control selective targeting of the decapping enzyme by forming distinct decapping complexes.
Title: Dcp2 C-terminal cis-binding elements control selective targeting of the decapping enzyme by forming distinct decapping complexes.
Suppressors of mRNA Decapping Defects Restore Growth Without Major Effects on mRNA Decay Rates or Abundance.
Title: Suppressors of mRNA Decapping Defects Restore Growth Without Major Effects on mRNA Decay Rates or Abundance.
Pby1 is a direct partner of the Dcp2 decapping enzyme.
Title: Pby1 is a direct partner of the Dcp2 decapping enzyme.
results suggest that Scd6 can (i) recruit Dhh1 to confer translational repression and (ii) activate mRNA decapping by Dcp2 with attendant degradation of specific mRNAs in vivo, in a manner dependent on the Scd6 LSm domain and modulated by Ccr4
Title: Conserved mRNA-granule component Scd6 targets Dhh1 to repress translation initiation and activates Dcp2-mediated mRNA decay in vivo.
Membrane-association of mRNA decapping factors, Dcp1, Dcp2, and Dhh1, is independent of stress in Saccharomyces cerevisiae.
Title: Membrane-association of mRNA decapping factors is independent of stress in budding yeast.
This recruitment requires Hrr25 kinase activity and the Dcp2 decapping enzyme, a core constituent of these RNP granules. Interestingly, the data indicate that this localization sequesters active Hrr25 away from the remainder of the cytoplasm and thereby shields this enzyme from the degradation machinery during these periods of stress.
Title: The Activity-Dependent Regulation of Protein Kinase Stability by the Localization to P-Bodies.
Results show that C-terminal domain of Dcp2 includes a set of conserved negative and positive regulatory elements reveal a pivotal role for the Dcp2 in mRNA decapping and lead to a new model for decapping enzyme regulation in vivo.
Title: Control of mRNA decapping by positive and negative regulatory elements in the Dcp2 C-terminal domain.
Local active site conformational changes are important for Dcp2 mRNA decapping activity.
Title: Active site conformational dynamics are coupled to catalysis in the mRNA decapping enzyme Dcp2.
Dcp1 links coactivators of mRNA decapping to Dcp2 by proline recognition
Title: Dcp1 links coactivators of mRNA decapping to Dcp2 by proline recognition.
show using NMR that cap binds conserved residues on both the catalytic and regulatory domains of Dcp2.
Title: A split active site couples cap recognition by Dcp2 to activation.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity	IEA Inferred from Electronic Annotation more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables hydrolase activity	IDA Inferred from Direct Assay more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables manganese ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in deadenylation-dependent decapping of nuclear-transcribed mRNA	IBA Inferred from Biological aspect of Ancestor more info
involved_in deadenylation-dependent decapping of nuclear-transcribed mRNA	IDA Inferred from Direct Assay more info	PubMed
involved_in deadenylation-dependent decapping of nuclear-transcribed mRNA	IEA Inferred from Electronic Annotation more info
involved_in deadenylation-dependent decapping of nuclear-transcribed mRNA	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in deadenylation-independent decapping of nuclear-transcribed mRNA	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription initiation by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in stress granule assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in stress granule assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in P-body	IBA Inferred from Biological aspect of Ancestor more info
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
located_in P-body	IEA Inferred from Electronic Annotation more info
part_of RNA decapping complex	IDA Inferred from Direct Assay more info	PubMed
part_of RNA decapping complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic side of membrane	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: decapping enzyme complex catalytic subunit

NP_014281.1

EC 3.6.1.62

Catalytic subunit of Dcp1p-Dcp2p decapping enzyme complex; removes 5' cap structure from mRNAs prior to their degradation; also enters nucleus and positively regulates transcription initiation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress; human homolog DCP2 complements yeast dcp2 thermosensitive mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

402652..405564 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182956.1 → NP_014281.1 TPA: decapping enzyme complex catalytic subunit [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014281.1

Status: REVIEWED

UniProtKB/Swiss-Prot

B0KZS6, B0KZU4, B0KZY0, B0KZY9, B0KZZ8, B0L007, D6W164, P53550, Q6LCS6

UniProtKB/TrEMBL

G2WLY8, N1P4C4

Conserved Domains (2) summary

cd03672
Location:103 → 242

Dcp2p; mRNA decapping enzyme 2 (Dcp2p), the catalytic subunit, and Dcp1p are the two components of the decapping enzyme complex. Decapping is a key step in both general and nonsense-mediated 5'->3' mRNA-decay pathways. Dcp2p contains an all-alpha helical ...

pfam05026
Location:17 → 99

DCP2; Dcp2, box A domain