BRO1 Bro1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: BRO1
Official Full Name: Bro1p
Primary source: SGD:S000006005
Locus tag: YPL084W
See related: AllianceGenome:SGD:S000006005; FungiDB:YPL084W; VEuPathDB:YPL084W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ASI6; LPF2; NPI3; VPS31
Summary: Enables deubiquitinase activator activity. Involved in several processes, including ATP export; intralumenal vesicle formation; and ubiquitin-dependent protein catabolic process. Located in cytoplasm. Is active in endosome and extracellular vesicle. Orthologous to human PTPN23 (protein tyrosine phosphatase non-receptor type 23). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See BRO1 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (394038..396572)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis.
Title: Bro1 stimulates Vps4 to promote intralumenal vesicle formation during multivesicular body biogenesis.
Novel mutations has been characterized in ESCRT-III Snf7 that trigger activation, and a novel role have been assigned to Bro1, the yeast ALIX ortholog, in Snf7 assembly.
Title: ESCRT-III activation by parallel action of ESCRT-I/II and ESCRT-0/Bro1 during MVB biogenesis.
overexpression of Bro1, particularly the V domain, prevented Rfu1 degradation in response to heat shock
Title: The ESCRT-III adaptor protein Bro1 controls functions of regulator for free ubiquitin chains 1 (Rfu1) in ubiquitin homeostasis.
Bro1 and other Bro1 family members function in parallel with ESCRT-0 to recognize and sort Ub-cargoes
Title: The yeast Alix homolog Bro1 functions as a ubiquitin receptor for protein sorting into multivesicular endosomes.
Results show that the pH-dependent localization of Rim20 is contingent on the amount of Bro1 in the cell.
Title: Intervention of Bro1 in pH-responsive Rim20 localization in Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables deubiquitinase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in ATP export	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intralumenal vesicle formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in intralumenal vesicle formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in late endosome to vacuole transport via multivesicular body sorting pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondria-associated ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein deubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein deubiquitination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein deubiquitination	IPI Inferred from Physical Interaction more info	PubMed
involved_in protein localization to endosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vacuolar transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
is_active_in endosome	IBA Inferred from Biological aspect of Ancestor more info
is_active_in endosome	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
is_active_in extracellular vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Bro1p

NP_015241.1

Cytoplasmic class E vacuolar protein sorting (VPS) factor; coordinates protein sorting and deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes; required for ESCRT-dependent transport of ubiquitinated cargo to the vacuole; localizes to extracellular vesicles during heat conditioning with proposed function in thermotolerance; orthologous to mammalian ESCRT-associated protein ALIX (PDCD6IP)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

394038..396572

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183898.1 → NP_015241.1 TPA: Bro1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015241.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W3T3, P48582, Q02823

UniProtKB/TrEMBL

A6ZWG7, C7GVE5, N1NVH3

Conserved Domains (2) summary

cd09237
Location:368 → 702

V_ScBro1_like; Protein-interacting V-domain of Saccharomyces cerevisiae Bro1 and related domains

cd09242
Location:5 → 347

BRO1_ScBro1_like; Protein-interacting, N-terminal, Bro1-like domain of Saccharomyces cerevisiae Bro1 and related proteins