madd-4 Protein madd-4 [Caenorhabditis elegans] - Gene

Summary

Go to the top of the page Help

Official Symbol: madd-4
Official Full Name: Protein madd-4
Primary source: WormBase:WBGene00009958
Locus tag: CELE_F53B6.2
See related: AllianceGenome:WB:WBGene00009958
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables signaling receptor binding activity. Involved in dorsal/ventral axon guidance and synapse organization. Located in extracellular space. Is expressed in blast cell; coelomocyte; and nervous system. Orthologous to human ADAMTS12 (ADAM metallopeptidase with thrombospondin type 1 motif 12); ADAMTSL1 (ADAMTS like 1); and ADAMTSL3 (ADAMTS like 3). [provided by Alliance of Genome Resources, Dec 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See madd-4 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 18

Sequence:: Chromosome: I; NC_003279.8 (8931033..8942292, complement)

Chromosome I - NC_003279.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003279.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Specific heparan sulfate modifications stabilize the synaptic organizer MADD-4/Punctin at Caenorhabditis elegans neuromuscular junctions.
Title: Specific heparan sulfate modifications stabilize the synaptic organizer MADD-4/Punctin at Caenorhabditis elegans neuromuscular junctions.
The HSPG syndecan is a core organizer of cholinergic synapses.
Title: The HSPG syndecan is a core organizer of cholinergic synapses.
Extracellular Matrix Muscle Arm Development Defective Protein Cooperates with the One Immunoglobulin Domain Protein To Suppress Precocious Synaptic Remodeling.
Title: Extracellular Matrix Muscle Arm Development Defective Protein Cooperates with the One Immunoglobulin Domain Protein To Suppress Precocious Synaptic Remodeling.
The Ig-like domain of Punctin/MADD-4 is the primary determinant for interaction with the ectodomain of neuroligin NLG-1.
Title: The Ig-like domain of Punctin/MADD-4 is the primary determinant for interaction with the ectodomain of neuroligin NLG-1.
we demonstrate that MADD-4 interacts with an EVA-1/UNC-40 co-receptor complex to attract cell extensions
Title: EVA-1 functions as an UNC-40 Co-receptor to enhance attraction to the MADD-4 guidance cue in Caenorhabditis elegans.
This study demonstrated that C. elegans Punctin Clusters GABA(A) Receptors via Neuroligin Binding and UNC-40/DCC Recruitment
Title: C. elegans Punctin Clusters GABA(A) Receptors via Neuroligin Binding and UNC-40/DCC Recruitment.
previously uncharacterized protein called MADD-4 is secreted by the dorsal and ventral nerve cords of the nematode C. elegans to attract sensory axons and muscle membrane extensions called muscle arms
Title: MADD-4 is a secreted cue required for midline-oriented guidance in Caenorhabditis elegans.
Punctin/madd-4, the C. elegans orthologue of mammalian punctin-1 and punctin-2, encodes neurally secreted isoforms that specify the excitatory or inhibitory identity of postsynaptic neuromuscular junction domains
Title: C. elegans Punctin specifies cholinergic versus GABAergic identity of postsynaptic domains.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

F53B6.2 (e-PCR)
- UniSTS:307689

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables signaling receptor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in dorsal/ventral axon guidance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in extracellular matrix organization	IEA Inferred from Electronic Annotation more info
involved_in synapse organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in axon	IEA Inferred from Electronic Annotation more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: Protein madd-4

NP_001250933.1

Confirmed by transcript evidence

NP_492405.3

Confirmed by transcript evidence

NP_871884.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003279.8 Reference assembly

Range

8931033..8942292 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_060004.5 → NP_492405.3 Protein madd-4 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_492405.3

Status: REVIEWED

UniProtKB/Swiss-Prot

I2HA89, P90884, Q8I4I1

Conserved Domains (2) summary

smart00408
Location:666 → 723

IGc2; Immunoglobulin C-2 Type

smart00209
Location:26 → 71

TSP1; Thrombospondin type 1 repeats
NM_182084.9 → NP_871884.1 Protein madd-4 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_871884.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P90884

Conserved Domains (3) summary

smart00408
Location:332 → 389

IGc2; Immunoglobulin C-2 Type

smart00209
Location:48 → 104

TSP1; Thrombospondin type 1 repeats

smart00410
Location:333 → 388

IG_like; Immunoglobulin like
NM_001264004.5 → NP_001250933.1 Protein madd-4 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001250933.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P90884

Conserved Domains (3) summary

smart00408
Location:664 → 721

IGc2; Immunoglobulin C-2 Type

smart00209
Location:26 → 71

TSP1; Thrombospondin type 1 repeats

smart00410
Location:665 → 720

IG_like; Immunoglobulin like