alg-2 Protein argonaute-2 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: alg-2
Official Full Name: Protein argonaute-2
Primary source: WormBase:WBGene00000106
Locus tag: CELE_T07D3.7
See related: AllianceGenome:WB:WBGene00000106
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables RNA binding activity and RNA endonuclease activity. Involved in embryo development; nematode larval development; and regulatory ncRNA-mediated gene silencing. Located in cytoplasm. Part of RISC complex. Is expressed in several structures, including germ line; gonad; hypodermis; neurons; and somatic cell. Human ortholog(s) of this gene implicated in alcohol dependence and glaucoma. Orthologous to several human genes including AGO2 (argonaute RISC catalytic component 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See alg-2 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 7

Sequence:: Chromosome: II; NC_003280.10 (873212..879885)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ALG-2 regulates IR-induced apoptosis via the mir-35 family of microRNAs.
Title: ALG-2/AGO-Dependent mir-35 Family Regulates DNA Damage-Induced Apoptosis Through MPK-1/ERK MAPK Signaling Downstream of the Core Apoptotic Machinery in Caenorhabditis elegans.
knockdown of alg-1 and alg-2 in germ cells results in few defects in oocyte maturation and ovulation.
Title: Functional analysis of microRNA pathway genes in the somatic gonad and germ cells during ovulation in C. elegans.
The slicing activity of ALG-2 is important to coordinate Dicer cleavage and thus is required for the formation of miRNA-induced silencing complex in C. elegans.
Title: The slicing activity of miRNA-specific Argonautes is essential for the miRNA pathway in C. elegans.
genetic and biochemical data hint at individual nonredundant functions for ALG-1 and ALG-2 in the processing of precursor miRNAs to mature miRNAs
Title: The Caenorhabditis elegans Argonautes ALG-1 and ALG-2: almost identical yet different.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

ha3206 (e-PCR)
- UniSTS:515665

T07D3.7 (e-PCR)
- UniSTS:310721

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity, producing 3'-phosphomonoesters	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables endoribonuclease activity, cleaving siRNA-paired mRNA	IDA Inferred from Direct Assay more info	PubMed
enables miRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables miRNA binding	IPI Inferred from Physical Interaction more info	PubMed
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables single-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in RISC complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryo development ending in birth or egg hatching	IGI Inferred from Genetic Interaction more info	PubMed
involved_in miRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in miRNA-mediated gene silencing by mRNA destabilization	IDA Inferred from Direct Assay more info	PubMed
involved_in nematode larval development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of RISC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RISC complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasmic ribonucleoprotein granule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Protein argonaute-2

NP_493837.1

Confirmed by transcript evidence

NP_871992.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

873212..879885

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_182192.6 → NP_871992.1 Protein argonaute-2 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_871992.1

Status: REVIEWED

UniProtKB/TrEMBL

O16720, Q3LTR7

Conserved Domains (5) summary

PLN03202
Location:67 → 859

PLN03202; protein argonaute; Provisional

cd02846
Location:274 → 395

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:440 → 866

Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...

pfam08699
Location:216 → 273

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:67 → 205

ArgoN; N-terminal domain of argonaute
NM_061436.6 → NP_493837.1 Protein argonaute-2 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_493837.1

Status: REVIEWED

UniProtKB/TrEMBL

Q3LTR7, Q86B39

Conserved Domains (5) summary

PLN03202
Location:48 → 840

PLN03202; protein argonaute; Provisional

cd02846
Location:255 → 376

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:421 → 847

Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...

pfam08699
Location:197 → 254

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:48 → 186

ArgoN; N-terminal domain of argonaute