Cers2 ceramide synthase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cers2provided by MGI
Official Full Name: ceramide synthase 2provided by MGI
Primary source: MGI:MGI:1924143
See related: Ensembl:ENSMUSG00000015714 AllianceGenome:MGI:1924143
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: TRH3; Lass2; 0610013I17Rik
Summary: Enables sphingosine N-acyltransferase activity. Involved in ceramide biosynthetic process and regulation of lipid metabolic process. Acts upstream of or within sphingolipid biosynthetic process. Predicted to be located in endoplasmic reticulum. Is expressed in cerebral cortex; limb; liver; neural tube; and skin. Orthologous to human CERS2 (ceramide synthase 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in liver adult (RPKM 73.3), placenta adult (RPKM 50.3) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 F2.1; 3 40.74 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (95222077..95230910)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (95314771..95323599)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

[LASS2/TMSG1 overexpression inhibits proliferation and promotes apoptosis of human lung cancer A549 cells possibly by upregulating ceramide and p38 MAPK to activate a signaling cascade].
Title: [LASS2/TMSG1 overexpression inhibits proliferation and promotes apoptosis of human lung cancer A549 cells possibly by upregulating ceramide and p38 MAPK to activate a signaling cascade].
Fatty acid transport protein 2 interacts with ceramide synthase 2 to promote ceramide synthesis.
Title: Fatty acid transport protein 2 interacts with ceramide synthase 2 to promote ceramide synthesis.
Ceramide Synthase 2 Null Mice Are Protected from Ovalbumin-Induced Asthma with Higher T Cell Receptor Signal Strength in CD4+ T Cells.
Title: Ceramide Synthase 2 Null Mice Are Protected from Ovalbumin-Induced Asthma with Higher T Cell Receptor Signal Strength in CD4+ T Cells.
LASS2 regulates hepatocyte steatosis by interacting with NDUFS2/OXPHOS related proteins.
Title: LASS2 regulates hepatocyte steatosis by interacting with NDUFS2/OXPHOS related proteins.
G-CSF signaling in CerS6 null bone marrow cells was not affected. In conclusion, very long chain ceramides are important for G-CSF signaling and translocation of G-CSF-R into detergent-resistant membranes.
Title: Role of ceramide synthase 2 in G-CSF signaling and G-CSF-R translocation into detergent-resistant membranes.
Our findings indicate that LASS2 plays an important role in the pathogenesis of diet-induced hepatic steatosis
Title: Hepatocyte-specific deletion of LASS2 protects against diet-induced hepatic steatosis and insulin resistance.
Gene expression analyses in livers of transgenic mouse mutants revealed that inactivation of CerS2 catalytic activity largely affects transcription of genes involved in lipid metabolism and cell division and is associated with the formation of hepatocellular carcinoma in 6 to 8-week-old mice.
Title: Inactivation of ceramide synthase 2 catalytic activity in mice affects transcription of genes involved in lipid metabolism and cell division.
Deficiency of CerS2 influences intestinal barrier function and the severity of experimental colitis and may represent a potential mechanism for inflammatory bowel disease pathogenesis.
Title: Ablation of ceramide synthase 2 exacerbates dextran sodium sulphate-induced colitis in mice due to increased intestinal permeability.
LASS2 plays an important role in efficient liver regeneration in response to partial hepatectomy.
Title: Liver-specific deletion of LASS2 delayed regeneration of mouse liver after partial hepatectomy.
Deletion of CerS2 strongly reduced very long-chain ceramides (Cer24:0, 24:1) but concomitantly increased long-chain ceramides and sphinganine in plasma and colon tissue. In naive CerS2(-/-) mice, the expression of tight junction proteins including ZO-1 was almost completely lost in the colon epithelium, leading to increased membrane permeability. Ceramide synthase 2 deficiency aggravates dextran induced colitis in mice.
Title: Ceramide synthase 2 deficiency aggravates AOM-DSS-induced colitis in mice: role of colon barrier integrity.

Submit: New GeneRIF Correction See all GeneRIFs (31)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1)
Targeted (7) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

UniSTS:234764 (e-PCR)
- UniSTS:234764

AU022152 (e-PCR)
- UniSTS:207535

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables N-acyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sphingosine N-acyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables sphingosine N-acyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables sphingosine N-acyltransferase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in ceramide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within ceramide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in ceramide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in ceramide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ceramide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of Schwann cell migration	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of Schwann cell migration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of Schwann cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of Schwann cell proliferation involved in axon regeneration	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of Schwann cell proliferation involved in axon regeneration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of axon regeneration	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of axon regeneration	ISO Inferred from Sequence Orthology more info
involved_in regulation of lipid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to immobilization stress	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within sphingolipid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISS Inferred from Sequence or Structural Similarity more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ceramide synthase 2

Names: LAG1 homolog, ceramide synthase 2; LAG1 longevity assurance homolog 2; TRAM homolog 3; longevity assurance homolog 2; sphingosine N-acyltransferase CERS2; translocating chain-associating membrane protein homolog 3; tumor metastasis-suppressor gene 1 protein; very-long-chain ceramide synthase CERS2

NP_001307421.1

EC 2.3.1.24

EC 2.3.1.297

NP_084065.1

EC 2.3.1.24

EC 2.3.1.297

XP_006502323.1

EC 2.3.1.24

EC 2.3.1.297

XP_030108738.1

EC 2.3.1.24

EC 2.3.1.297

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001320492.1 → NP_001307421.1 ceramide synthase 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.

Source sequence(s)

AC140190, AK159325, BQ176425, BY773771, DN176473

Consensus CDS

CCDS17612.1

UniProtKB/Swiss-Prot

Q3TXC5, Q924Z4, Q9DCN6

Conserved Domains (2) summary

smart00724
Location:131 → 332

TLC; TRAM, LAG1 and CLN8 homology domains

cd00086
Location:71 → 128

homeodomain; Homeodomain; DNA binding domains involved in the transcriptional regulation of key eukaryotic developmental processes; may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner.
NM_029789.2 → NP_084065.1 ceramide synthase 2

See identical proteins and their annotated locations for NP_084065.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein

Source sequence(s)

AC140190, AK159325, BQ176425, CD564737

Consensus CDS

CCDS17612.1

UniProtKB/Swiss-Prot

Q3TXC5, Q924Z4, Q9DCN6

Related

ENSMUSP00000015858.5, ENSMUST00000015858.12

Conserved Domains (2) summary

smart00724
Location:131 → 332

TLC; TRAM, LAG1 and CLN8 homology domains

cd00086
Location:71 → 128

homeodomain; Homeodomain; DNA binding domains involved in the transcriptional regulation of key eukaryotic developmental processes; may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner.

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

95222077..95230910

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006502260.3 → XP_006502323.1 ceramide synthase 2 isoform X1

See identical proteins and their annotated locations for XP_006502323.1

UniProtKB/Swiss-Prot

Q3TXC5, Q924Z4, Q9DCN6

Conserved Domains (2) summary

smart00724
Location:131 → 332

TLC; TRAM, LAG1 and CLN8 homology domains

cd00086
Location:71 → 128

homeodomain; Homeodomain; DNA binding domains involved in the transcriptional regulation of key eukaryotic developmental processes; may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner.
XM_030252878.1 → XP_030108738.1 ceramide synthase 2 isoform X1

UniProtKB/Swiss-Prot

Q3TXC5, Q924Z4, Q9DCN6

Conserved Domains (2) summary

smart00724
Location:131 → 332

TLC; TRAM, LAG1 and CLN8 homology domains

cd00086
Location:71 → 128

homeodomain; Homeodomain; DNA binding domains involved in the transcriptional regulation of key eukaryotic developmental processes; may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner.