Trim45 tripartite motif-containing 45 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Trim45provided by MGI
Official Full Name: tripartite motif-containing 45provided by MGI
Primary source: MGI:MGI:1918187
See related: Ensembl:ENSMUSG00000033233 AllianceGenome:MGI:1918187
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 4921530N01Rik
Summary: Predicted to enable ubiquitin protein ligase activity. Acts upstream of or within bone development. Predicted to be located in cytosol and intercellular bridge. Predicted to be active in cytoplasm and nucleoplasm. Is expressed in several structures, including axial skeleton; central nervous system; genitourinary system; and sensory organ. Orthologous to human TRIM45 (tripartite motif containing 45). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in testis adult (RPKM 4.9), subcutaneous fat pad adult (RPKM 3.3) and 21 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 F2.2; 3 43.85 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (100828516..100844241)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (100921203..100936925)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy.
Title: TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy.
TRIM45 causes neuronal damage by aggravating microglia-mediated neuroinflammation upon cerebral ischemia and reperfusion injury.
Title: TRIM45 causes neuronal damage by aggravating microglia-mediated neuroinflammation upon cerebral ischemia and reperfusion injury.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:234778

MHAa35c5.seq (e-PCR)
- UniSTS:125160

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within bone development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein K48-linked ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein K48-linked ubiquitination	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in intercellular bridge	IEA Inferred from Electronic Annotation more info
located_in intercellular bridge	ISO Inferred from Sequence Orthology more info
is_active_in nucleoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase TRIM45

Names: RING finger protein 99; tripartite motif-containing protein 45

NP_001159424.1

EC 2.3.2.27

NP_001159425.1

EC 2.3.2.27

NP_919324.2

EC 2.3.2.27

XP_011238402.1

EC 2.3.2.27

XP_030108447.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001165952.1 → NP_001159424.1 E3 ubiquitin-protein ligase TRIM45 isoform b

See identical proteins and their annotated locations for NP_001159424.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 3' UTR and lacks an alternate exon that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. Both variants 2 and 3 encode the same isoform.

Source sequence(s)

AK150574, AL669872, BY518289

Consensus CDS

CCDS17678.1

UniProtKB/Swiss-Prot

E9Q2K9, Q3U5H4, Q6PFY8, Q8BVT5

UniProtKB/TrEMBL

A2ACL2

Related

ENSMUSP00000102593.3, ENSMUST00000106980.9

Conserved Domains (4) summary

smart00557
Location:399 → 497

IG_FLMN; Filamin-type immunoglobulin domains

cd00021
Location:189 → 226

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

pfam00630
Location:397 → 493

Filamin; Filamin/ABP280 repeat

cl23765
Location:237 → 357

iSH2_PI3K_IA_R; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunits
NM_001165953.1 → NP_001159425.1 E3 ubiquitin-protein ligase TRIM45 isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a).

Source sequence(s)

AK076600, AK150574, AL669872, BC057358

Consensus CDS

CCDS51018.1

UniProtKB/Swiss-Prot

Q6PFY8

Related

ENSMUSP00000115669.3, ENSMUST00000134993.3

Conserved Domains (4) summary

smart00557
Location:399 → 497

IG_FLMN; Filamin-type immunoglobulin domains

cd00021
Location:189 → 226

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

pfam00630
Location:397 → 493

Filamin; Filamin/ABP280 repeat

cl23765
Location:237 → 357

iSH2_PI3K_IA_R; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunits
NM_194343.2 → NP_919324.2 E3 ubiquitin-protein ligase TRIM45 isoform b

See identical proteins and their annotated locations for NP_919324.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate exon that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. Both variants 2 and 3 encode the same isoform.

Source sequence(s)

AK150574, BC057358

Consensus CDS

CCDS17678.1

UniProtKB/Swiss-Prot

E9Q2K9, Q3U5H4, Q6PFY8, Q8BVT5

UniProtKB/TrEMBL

A2ACL2

Related

ENSMUSP00000043389.7, ENSMUST00000037409.13

Conserved Domains (4) summary

smart00557
Location:399 → 497

IG_FLMN; Filamin-type immunoglobulin domains

cd00021
Location:189 → 226

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

pfam00630
Location:397 → 493

Filamin; Filamin/ABP280 repeat

cl23765
Location:237 → 357

iSH2_PI3K_IA_R; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunits

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

100828516..100844241

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011240100.3 → XP_011238402.1 E3 ubiquitin-protein ligase TRIM45 isoform X1

Conserved Domains (3) summary

smart00502
Location:65 → 185

BBC; B-Box C-terminal domain

pfam00630
Location:225 → 320

Filamin; Filamin/ABP280 repeat

cd19785
Location:16 → 58

Bbox2_TRIM45_C-X; B-box-type 2 zinc finger found in tripartite motif-containing protein 45 (TRIM45) and similar proteins
XM_030252587.2 → XP_030108447.1 E3 ubiquitin-protein ligase TRIM45 isoform X2

Conserved Domains (3) summary

smart00502
Location:65 → 185

BBC; B-Box C-terminal domain

smart00557
Location:227 → 325

IG_FLMN; Filamin-type immunoglobulin domains

cd19785
Location:16 → 58

Bbox2_TRIM45_C-X; B-box-type 2 zinc finger found in tripartite motif-containing protein 45 (TRIM45) and similar proteins