DHH1 DExD/H-box ATP-dependent RNA helicase DHH1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DHH1
Official Full Name: DExD/H-box ATP-dependent RNA helicase DHH1
Primary source: SGD:S000002319
Locus tag: YDL160C
See related: AllianceGenome:SGD:S000002319; FungiDB:YDL160C; VEuPathDB:YDL160C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables ATP hydrolysis activity; chromatin binding activity; and mRNA binding activity. Involved in several processes, including membraneless organelle assembly; post-transcriptional regulation of gene expression; and pseudohyphal growth. Located in P-body; cytoplasmic side of membrane; and cytoplasmic stress granule. Orthologous to human DDX6 (DEAD-box helicase 6). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See DHH1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (170410..171930, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

mRNA decapping activators Pat1 and Dhh1 regulate transcript abundance and translation to tune cellular responses to nutrient availability.
Title: mRNA decapping activators Pat1 and Dhh1 regulate transcript abundance and translation to tune cellular responses to nutrient availability.
Both the RNA-binding ability and helicase activity of Dhh1 are indispensable to promote Atg1 translation and autophagy.
Title: Dhh1 promotes autophagy-related protein translation during nitrogen starvation.
Based on the data the authors hypothesized that Dhh1 is responsible for the highly selective inhibition of POM34 mRNA by the SESA (Smy2, Eap1, Scp160, Asc1) protein network.
Title: Dhh1 is a member of the SESA network.
Our results define the roles of Pat1, Lsm1, and Dhh1 in decapping of general mRNAs and suggest that these factors may monitor mRNA translation and target unique features of individual mRNAs.
Title: General decapping activators target different subsets of inefficiently translated mRNAs.
results suggest that Scd6 can (i) recruit Dhh1 to confer translational repression and (ii) activate mRNA decapping by Dcp2 with attendant degradation of specific mRNAs in vivo, in a manner dependent on the Scd6 LSm domain and modulated by Ccr4
Title: Conserved mRNA-granule component Scd6 targets Dhh1 to repress translation initiation and activates Dcp2-mediated mRNA decay in vivo.
All Dhh1-dependent mRNAs, viral and cellular, share key common features. First, they contain long and highly structured CDSs, including a region located around nucleotide 70 after the translation initiation site; second, they are directly bound by Dhh1 with a specific binding distribution; and third, complementary experimental approaches suggest that they are activated by Dhh1 at the translation initiation step
Title: A novel translational control mechanism involving RNA structures within coding sequences.
Here, the authors show that in budding yeast, mutations in the DEAD-box ATPase Dhh1 that prevent ATP hydrolysis, or that affect the interaction between Dhh1 and Not1, the central scaffold of the CCR4-NOT complex and an activator of the Dhh1 ATPase, prevent processing bodies disassembly in vivo.
Title: ATPase activity of the DEAD-box protein Dhh1 controls processing body formation.
findings show that the mutations in ATPase A and B motif (K96R, D195A) affected the growth at non-permissive temperature, the mating processes including the Ste12 expression, and the P-body accumulation
Title: Mutational analysis of the RNA helicase Dhh1 in Ste12 expression and yeast mating.
These results suggest that after localization to the bud tip, a portion of the localized ASH1 mRNA becomes translationally inactive because of binding of Dhh1 and Puf6 to the 5'- and 3'-UTRs of ASH1 mRNA.
Title: Binding of DEAD-box helicase Dhh1 to the 5'-untranslated region of ASH1 mRNA represses localized translation of ASH1 in yeast cells.
Data indicate that RNA helicase RCK family member Dhh1 and the mRNA decay pathway coordinately repress the autophagy gene expression.
Title: A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy.

Submit: New GeneRIF Correction See all GeneRIFs (25)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA helicase activity	IEA Inferred from Electronic Annotation more info
enables RNA helicase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables mRNA binding	HDA more info	PubMed
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in P-body assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in P-body assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in P-body assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to glucose starvation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to glucose starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to nitrogen starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in deadenylation-dependent decapping of nuclear-transcribed mRNA	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in filamentous growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in invasive filamentous growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in mRNA transport	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of translation	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of translational elongation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of translational elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pseudohyphal growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cytoplasmic mRNA processing body assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cytoplasmic mRNA processing body assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in response to pheromone triggering conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in stress granule assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in stress granule assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in P-body	IBA Inferred from Biological aspect of Ancestor more info
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
located_in P-body	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic side of membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic stress granule	HDA more info	PubMed
is_active_in cytoplasmic stress granule	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DExD/H-box ATP-dependent RNA helicase DHH1

NP_010121.1

EC 3.6.4.13

Cytoplasmic DEAD-box helicase and mRNA decapping activator; interacts with decapping and deadenylase complexes to coordinate mRNA decapping and decay; regulates general translational repression; translational activator of select mRNAs during filamentous growth, mating and autophagy; cooperates with Ngr1p to promote specific mRNA decay; ATP- and RNA-bound form promotes processing body assembly, while ATPase stimulation by Not1p promotes disassembly; forms cytoplasmic foci on replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

170410..171930 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180220.1 → NP_010121.1 TPA: DExD/H-box ATP-dependent RNA helicase DHH1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010121.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZXG9, D6VRJ0, P39517

UniProtKB/TrEMBL

B3LH47, B5VFB0, C7GL09, C8Z6E3, G2WC03, N1P7U0

Conserved Domains (2) summary

cd18787
Location:258 → 387

SF2_C_DEAD; C-terminal helicase domain of the DEAD box helicases

cd17940
Location:48 → 248

DEADc_DDX6; DEAD-box helicase domain of DEAD box protein 6