Atp4a ATPase, H+/K+ exchanging, gastric, alpha polypeptide [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Atp4aprovided by MGI
Official Full Name: ATPase, H+/K+ exchanging, gastric, alpha polypeptideprovided by MGI
Primary source: MGI:MGI:88113
See related: Ensembl:ENSMUSG00000005553 AllianceGenome:MGI:88113
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Enables P-type potassium:proton transporter activity. Acts upstream of or within pH reduction; regulation of proton transport; and response to xenobiotic stimulus. Located in plasma membrane. Is expressed in body of stomach and stomach epithelium. Orthologous to human ATP4A (ATPase H+/K+ transporting subunit alpha). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Restricted expression toward stomach adult (RPKM 485.0) See more
Orthologs: human all
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Genomic context

Location:: 7 B1; 7 19.2 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (30411634..30424959)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (30712209..30725534)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dynamic characterization of intestinal metaplasia in the gastric corpus mucosa of Atp4a-deficient mice.
Title: Dynamic characterization of intestinal metaplasia in the gastric corpus mucosa of Atp4a-deficient mice.
Two genes, Atp4a and Pop4, fulfilled the criteria from our candidate gene approaches. The knockdown of both genes in MIN6 cells led to decreased glucose-stimulated insulin secretion, indicating a regulatory role of both genes in insulin secretion, thereby possibly contributing to the phenotype linked to Nbg7p
Title: Two Novel Candidate Genes for Insulin Secretion Identified by Comparative Genomics of Multiple Backcross Mouse Populations.
the effect of GOAT on gastric acid secretion and expression of ghrelin in vitro, were investigated.
Title: The effect of ghrelin O-acyltransferase inhibitor on gastric H(+)-K(+)-ATPase activity and GOAT/ghrelin system in gastric mucosal cells in vitro.
ENaC activity in the cortical collecting duct of HKalpha1 H(+),K(+)-ATPase knockout mice is uncoupled from Na(+) intake.
Title: ENaC activity in the cortical collecting duct of HKα(1) H(+),K(+)-ATPase knockout mice is uncoupled from Na(+) intake.
Point mutation in Atp4a (or Atp4a-/- knockout) results in achlorhydria and leads to iron-deficiency anemia; thus, functioning Atp4a is necessary for absorption of dietary iron leading to effective erythropoiesis.
Title: Mutation of the gastric hydrogen-potassium ATPase alpha subunit causes iron-deficiency anemia in mice.
The metabolism of H,K-ATPases in in collecting ducts of normal and potassium-depleted mice reflect the functional expression of gastric and colonic H,K-ATPase, respectively.
Title: Molecular identification of Sch28080-sensitive K-ATPase activities in the mouse kidney.
Both HKalpha(1) and HKalpha(2) contribute to proton secretion in both A-type and B-type intercalated cells in animals fed a normal diet.
Title: Impaired acid secretion in cortical collecting duct intercalated cells from H-K-ATPase-deficient mice: role of HKalpha isoforms.
Prevention of autoimmune gastritis requires the extra-thymic purging of highly autoaggressive H(+)/K(+) ATPase-specific T cells to produce a T-cell repertoire that is more susceptible to the suppressive activity of regulatory T cells.
Title: Prevention of autoimmune gastritis in mice requires extra-thymic T-cell deletion and suppression by regulatory T cells.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (2)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables P-type potassium:proton transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables P-type potassium:proton transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables P-type sodium:potassium-exchanging transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables magnesium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables potassium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in intracellular potassium ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular sodium ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within pH reduction	IDA Inferred from Direct Assay more info	PubMed
involved_in potassium ion import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in potassium ion transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in proton transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in proton transmembrane transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of proton transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to xenobiotic stimulus	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to xenobiotic stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sodium ion export across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
part_of potassium:proton exchanging ATPase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: potassium-transporting ATPase alpha chain 1

Names: ATPase, H+/K+ exchanging, alpha polypeptide; ATPase, H+/K+ transporting, alpha polypeptide; H+/K+-ATPase alpha; H+K+-transporting alpha 1; gastric H(+)/K(+) ATPase subunit alpha; proton pump

NP_001277556.1

EC 7.2.2.19

NP_061201.2

EC 7.2.2.19

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001290627.1 → NP_001277556.1 potassium-transporting ATPase alpha chain 1 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC167978

Consensus CDS

CCDS71932.1

UniProtKB/Swiss-Prot

E9QNX7, Q64436, Q9CV46

Related

ENSMUSP00000005692.7, ENSMUST00000005692.14

Conserved Domains (7) summary

smart00831
Location:50 → 124

Cation_ATPase_N; Cation transporter/ATPase, N-terminus

COG4087
Location:616 → 742

COG4087; Soluble P-type ATPase [General function prediction only]

TIGR01106
Location:42 → 1034

ATPase-IIC_X-K; sodium or proton efflux -- potassium uptake antiporter, P-type ATPase, alpha subunit

pfam00122
Location:156 → 375

E1-E2_ATPase; E1-E2 ATPase

pfam00689
Location:809 → 1018

Cation_ATPase_C; Cation transporting ATPase, C-terminus

pfam09040
Location:2 → 27

H-K_ATPase_N; Gastric H+/K+-ATPase, N terminal domain

pfam13246
Location:437 → 531

Cation_ATPase; Cation transport ATPase (P-type)
NM_018731.3 → NP_061201.2 potassium-transporting ATPase alpha chain 1 isoform 2

See identical proteins and their annotated locations for NP_061201.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

Source sequence(s)

AC167978

Consensus CDS

CCDS52182.1

UniProtKB/TrEMBL

Q91WH7

Related

ENSMUSP00000131964.2, ENSMUST00000170371.2

Conserved Domains (7) summary

smart00831
Location:50 → 124

Cation_ATPase_N; Cation transporter/ATPase, N-terminus

TIGR01106
Location:42 → 1025

ATPase-IIC_X-K; sodium or proton efflux -- potassium uptake antiporter, P-type ATPase, alpha subunit

pfam00122
Location:156 → 375

E1-E2_ATPase; E1-E2 ATPase

pfam00689
Location:800 → 1009

Cation_ATPase_C; Cation transporting ATPase, C-terminus

pfam09040
Location:2 → 27

H-K_ATPase_N; Gastric H+/K+-ATPase, N terminal domain

pfam12710
Location:611 → 727

HAD; haloacid dehalogenase-like hydrolase

pfam13246
Location:437 → 531

Cation_ATPase; Cation transport ATPase (P-type)