rfl-1 NEDD8-activating enzyme E1 catalytic subunit [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: rfl-1
Official Full Name: NEDD8-activating enzyme E1 catalytic subunit
Primary source: WormBase:WBGene00004341
Locus tag: CELE_F11H8.1
See related: AllianceGenome:WB:WBGene00004341
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable NEDD8 activating enzyme activity. Involved in negative regulation of DNA damage response, signal transduction by p53 class mediator and protein neddylation. Located in cytoplasm and nucleus. Is expressed in several structures, including hypodermis; intestine; nervous system; pharynx; and vulval cell. Orthologous to human UBA3 (ubiquitin like modifier activating enzyme 3). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See rfl-1 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 8

Sequence:: Chromosome: III; NC_003281.10 (7021885..7023652)

Chromosome III - NC_003281.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003281.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Depletion of the strongest specific suppressors rescued the early embryonic cell division defects in rfl-1 mutants; depletion of the two strongest specific enhancers did not affect the early embryonic cell division defects
Title: Using RNA interference to identify specific modifiers of a temperature-sensitive, embryonic-lethal mutation in the Caenorhabditis elegans ubiquitin-like Nedd8 protein modification pathway E1-activating gene rfl-1.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F11H8.1 (e-PCR)
- UniSTS:306017

F11H8.3 (e-PCR)
- UniSTS:309699

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables NEDD8 activating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
enables NEDD8 activating enzyme activity	IEA Inferred from Electronic Annotation more info
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-like modifier activating enzyme activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in negative regulation of DNA damage response, signal transduction by p53 class mediator	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein neddylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein neddylation	IEA Inferred from Electronic Annotation more info
involved_in protein neddylation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: NEDD8-activating enzyme E1 catalytic subunit

NP_498534.2

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003281.10 Reference assembly

Range

7021885..7023652

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_066133.8 → NP_498534.2 NEDD8-activating enzyme E1 catalytic subunit [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_498534.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q19360

Conserved Domains (3) summary

cd01488
Location:44 → 341

Uba3_RUB; Ubiquitin activating enzyme (E1) subunit UBA3. UBA3 is part of the heterodimeric activating enzyme (E1), specific for the Rub family of ubiquitin-like proteins (Ubls). E1 enzymes are part of a conjugation cascade to attach Ub or Ubls, covalently to ...

pfam00899
Location:33 → 214

ThiF; ThiF family

pfam08825
Location:349 → 429

E2_bind; E2 binding domain