mbt mushroom bodies tiny [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: mbtprovided by FlyBase
Official Full Name: mushroom bodies tinyprovided by FlyBase
Primary source: FLYBASE:FBgn0025743
Locus tag: Dmel_CG18582
See related: AllianceGenome:FB:FBgn0025743
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-EST:fe1G7; CG18582; Dmel\CG18582; DmMBT; DmPAK2; dPAK2; Mbt; PAK; Pak2; PAK2; Ste20; STE20
Summary: Enables ATP binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including negative regulation of cell size; nervous system development; and positive regulation of compound eye photoreceptor development. Located in adherens junction and cytoplasm. Colocalizes with plasma membrane. Is expressed in adult head; neuroblasts; organism; and ovary. Used to study Parkinson's disease. Human ortholog(s) of this gene implicated in clear cell renal cell carcinoma. Orthologous to several human genes including PAK4 (p21 (RAC1) activated kinase 4). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 14F2-14F2; 1-54 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (16610685..16614856)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (16504718..16508889)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rap1, Canoe and Mbt regulated epithelial morphogenesis by coupling stabilization of the adherens junction (AJ) protein E-Cadherin and Bazooka retention at the zonula adherens
Title: Rap1, Canoe and Mbt cooperate with Bazooka to promote zonula adherens assembly in the fly photoreceptor.
miR-252 directly targeted mbt, encoding a p21-activated kinase, to repress its expression. Notably, co-overexpression of mbt rescued the developmental and growth defects associated with miR-252 overexpression, indicating that mbt is a biologically relevant target of miR-252
Title: MicroRNA miR-252 targets mbt to control the developmental growth of Drosophila.
Pak4 regulates adherens junction accumulation. Pak4 promotes Par3 retention at the zonula adherens.
Title: Pak4 Is Required during Epithelial Polarity Remodeling through Regulating AJ Stability and Bazooka Retention at the ZA.
Mbt regulates neuroblast number throughout postembryonic brain development.
Title: The p21-activated kinase Mbt is a component of the apical protein complex in central brain neuroblasts and controls cell proliferation.
Activation of the human Mbt homologue PAK4 leads to anchorage-independent growth and provide a functional link between a PAK protein and the cadherin-catenin complex.
Title: The Drosophila p21-activated kinase Mbt modulates DE-cadherin-mediated cell adhesion by phosphorylation of Armadillo.
role for Mbt as a downstream effector of Cdc42 in photoreceptor cell morphogenesis
Title: Mbt, a Drosophila PAK protein, combines with Cdc42 to regulate photoreceptor cell morphogenesis.
Constitutive activation of Mbt not only disturbs the actin cytoskeleton but also affects adherens junction organisation indicating a requirement of the protein in cell adhesion dependent processes during photoreceptor cell differentiation.
Title: The Drosophila p21 activated kinase Mbt regulates the actin cytoskeleton and adherens junctions to control photoreceptor cell morphogenesis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in compound eye development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in compound eye photoreceptor cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cytoskeleton organization	TAS Traceable Author Statement more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in mushroom body development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell size	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of compound eye photoreceptor development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of developmental growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell-cell adhesion mediated by cadherin	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in adherens junction	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: mushroom bodies tiny

Names: CG18582-PA; anon-fast-evolving-1G7; mbt-PA

NP_523375.2

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004354.4 Reference assembly

Range

16610685..16614856

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_078651.4 → NP_523375.2 mushroom bodies tiny [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523375.2

Status: REVIEWED

UniProtKB/Swiss-Prot

O96372, Q960J8, Q9TYH2, Q9VXE5

UniProtKB/TrEMBL

B5RIT0

Related

FBpp0074060

Conserved Domains (3) summary

smart00220
Location:372 → 619

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd01093
Location:9 → 54

CRIB_PAK_like; PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the ...

cd06648
Location:360 → 620

STKc_PAK_II; Catalytic domain of the Serine/Threonine Kinase, Group II p21-activated kinase