HRQ1 ATP-dependent 3'-5' DNA helicase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: HRQ1
Official Full Name: ATP-dependent 3'-5' DNA helicase
Primary source: SGD:S000002699
Locus tag: YDR291W
See related: AllianceGenome:SGD:S000002699; FungiDB:YDR291W; VEuPathDB:YDR291W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables 3'-5' DNA helicase activity; DNA binding activity; and enzyme activator activity. Involved in DNA repair; chromosome organization; and regulation of telomere maintenance. Acts upstream of or within error-free postreplication DNA repair and positive regulation of nuclease activity. Located in nucleus. Used to study Baller-Gerold syndrome; Rothmund-Thomson syndrome; breast cancer; and rapadilino syndrome. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See HRQ1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1039728..1042961)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Genetic and Physical Interactomes of the Saccharomyces cerevisiae Hrq1 Helicase.
Title: The Genetic and Physical Interactomes of the Saccharomyces cerevisiae Hrq1 Helicase.
Comprehensive Synthetic Genetic Array Analysis of Alleles That Interact with Mutation of the Saccharomyces cerevisiae RecQ Helicases Hrq1 and Sgs1.
Title: Comprehensive Synthetic Genetic Array Analysis of Alleles That Interact with Mutation of the Saccharomyces cerevisiae RecQ Helicases Hrq1 and Sgs1.
Pif1DeltaN was still able to synergize with the Hrq1 helicase to inhibit telomerase activity in vitro, similar to full-length Pif1. These data impact our understanding of Pif1 helicase evolution and the roles of these enzymes in the maintenance of genome integrity.
Title: The Biochemical Activities of the Saccharomyces cerevisiae Pif1 Helicase Are Regulated by Its N-Terminal Domain.
results suggest that Hrq1 and Pif1 interact and that their concerted activities ensure proper telomere length homeostasis in vivo
Title: The Saccharomyces cerevisiae Hrq1 and Pif1 DNA helicases synergistically modulate telomerase activity in vitro.
These results establish the importance of DNA sequence in Hrq1 helicase activity, and the absence of Hrq1 strand annealing activity explains the previously identified discrepancies between S. cerevisiae Hrq1 and human RecQ4.
Title: Saccharomyces cerevisiae Hrq1 helicase activity is affected by the sequence but not the length of single-stranded DNA.
Overexpression of Hrq1K318A helicase-deficient protein rendered mutant cells more sensitive to 4-nitroquinoline-1-oxide and cisplatin, suggesting that helicase activity is required for the proper function of Hrq1 in nucleotide excision repair.
Title: Hrq1 facilitates nucleotide excision repair of DNA damage induced by 4-nitroquinoline-1-oxide and cisplatin in Saccharomyces cerevisiae.
Hrq1 acts catalytically to protect against DNA interstrand crosslinks and affects telomere length by a noncatalytic mechanism.
Title: Hrq1, a homolog of the human RecQ4 helicase, acts catalytically and structurally to promote genome integrity.
These results suggest that Hrq1 is involved in recombination and DNA repair pathways in Saccharomyces cerevisiae independent of Sgs1.
Title: Hrq1 functions independently of Sgs1 to preserve genome integrity in Saccharomyces cerevisiae.
these results indicate that Hrq1 has vigorous helicase activity that deserves further characterization to expand our understanding of RecQ helicases.
Title: Saccharomyces cerevisiae Hrq1 requires a long 3'-tailed DNA substrate for helicase activity.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3'-5' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3'-5' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables enzyme activator activity	IDA Inferred from Direct Assay more info	PubMed
enables forked DNA-dependent helicase activity	IEA Inferred from Electronic Annotation more info
enables four-way junction helicase activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables single-stranded 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables telomeric DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within error-free postreplication DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in interstrand cross-link repair	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within interstrand cross-link repair	IDA Inferred from Direct Assay more info	PubMed
involved_in interstrand cross-link repair	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleotide-excision repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleotide-excision repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nucleotide-excision repair	IPI Inferred from Physical Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of nuclease activity	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of nuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of telomere maintenance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ATP-dependent 3'-5' DNA helicase

NP_010577.3

EC 3.6.4.12

3'-5' DNA helicase of the conserved RecQ family; involved in Pso2p-mediated interstrand crosslink repair; acts with Rad4p in nucleotide-excision repair; binds telomeres, modulates telomerase activity with Pif1p and inhibits telomere addition to dsDNA breaks; role in telomerase-independent telomere maintenance; lacks ssDNA annealing and strand exchange activities; human RecQL4, a structural and functional homolog, is involved in Rothmund-Thomson, Baller-Gerold and RAPADILINO syndromes

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1039728..1042961

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180599.3 → NP_010577.3 TPA: ATP-dependent 3'-5' DNA helicase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010577.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VSS0, Q05549

UniProtKB/TrEMBL

A6ZYN9, B3LG04, C7GQU1, C8Z5K3, G2WB29, N1PAH1

Conserved Domains (1) summary

COG1205
Location:143 → 1077

YprA; ATP-dependent helicase YprA, contains C-terminal metal-binding DUF1998 domain [Replication, recombination and repair]