SCC2 cohesin-loading factor complex subunit SCC2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SCC2
Official Full Name: cohesin-loading factor complex subunit SCC2
Primary source: SGD:S000002588
Locus tag: YDR180W
See related: AllianceGenome:SGD:S000002588; FungiDB:YDR180W; VEuPathDB:YDR180W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to kinetochore binding activity. Involved in chromosome organization; establishment of protein localization to chromatin; and replication-born double-strand break repair via sister chromatid exchange. Located in several cellular components, including 2-micrometer circle DNA; chromatin; and cytosol. Part of SMC loading complex. Used to study cancer. Human ortholog(s) of this gene implicated in Cornelia de Lange syndrome and Cornelia de Lange syndrome 1. Orthologous to human NIPBL (NIPBL cohesin loading factor). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SCC2 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (821295..825776)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Conformational dynamics of cohesin/Scc2 loading complex are regulated by Smc3 acetylation and ATP binding.
Title: Conformational dynamics of cohesin/Scc2 loading complex are regulated by Smc3 acetylation and ATP binding.
A model for Scc2p stimulation of cohesin's ATPase and its inhibition by acetylation of Smc3p.
Title: A model for Scc2p stimulation of cohesin's ATPase and its inhibition by acetylation of Smc3p.
Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin-dependent chromatin loops.
Title: Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin-dependent chromatin loops.
Recruitment of Scc2/4 to double-strand breaks depends on gammaH2A and DNA end resection.
Title: Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resection.
Folding of cohesin's coiled coil is important for Scc2/4-induced association with chromosomes.
Title: Folding of cohesin's coiled coil is important for Scc2/4-induced association with chromosomes.
Chromosome loading of cohesin depends on conserved residues in Scc3.
Title: Chromosome loading of cohesin depends on conserved residues in Scc3.
A conserved ATP- and Scc2/4-dependent activity for cohesin in tethering DNA molecules.
Title: A conserved ATP- and Scc2/4-dependent activity for cohesin in tethering DNA molecules.
The authors show here that Scc2 also actively maintains cohesin on chromosomes during G1 in S. cerevisiae cells. It does so by blocking a Wapl-independent release reaction that requires opening the cohesin ring at its Smc3/Scc1 interface as well as the D loop of Smc1's ATPase.
Title: Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1.
Here we reveal a dual role of RSC in cohesin loading. RSC acts as a chromatin receptor that recruits Scc2-Scc4 by a direct protein interaction independent of chromatin remodeling. In addition, chromatin remodeling is required to generate a nucleosome-free region that is the substrate for cohesin loading.
Title: A Role for Chromatin Remodeling in Cohesin Loading onto Chromosomes.
Nipbl seems to have also additional roles, for instance as transcription factor.This chapter summarizes our current knowledge on kollerin function and the recent studies on the genomic localization of Scc2, highlighting and critically discussing controversial data.
Title: Resolving the Genomic Localization of the Kollerin Cohesin-Loader Complex.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables chromatin binding	IBA Inferred from Biological aspect of Ancestor more info
enables chromatin binding	IEA Inferred from Electronic Annotation more info
contributes_to kinetochore binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in double-strand break repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic sister chromatid cohesion	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of mitotic sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of protein localization to chromatin	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of protein localization to chromatin	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic chromosome condensation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to chromatin	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rDNA chromatin condensation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in replication-born double-strand break repair via sister chromatid exchange	IBA Inferred from Biological aspect of Ancestor more info
involved_in replication-born double-strand break repair via sister chromatid exchange	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA gene clustering	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in 2-micrometer circle DNA	IDA Inferred from Direct Assay more info	PubMed
part_of SMC loading complex	IPI Inferred from Physical Interaction more info	PubMed
part_of Scc2-Scc4 cohesin loading complex	IBA Inferred from Biological aspect of Ancestor more info
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: cohesin-loading factor complex subunit SCC2

NP_010466.3

Subunit of cohesin loading factor (Scc2p-Scc4p); a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; promotes gene expression program that supports translational fidelity; evolutionarily-conserved adherin; relocalizes to cytosol in response to hypoxia; human disorder Cornelia de Lange syndrome is caused by mutations in NIPBL, the human ortholog of SCC2; conserved role in NHEJ

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

821295..825776

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180488.3 → NP_010466.3 TPA: cohesin-loading factor complex subunit SCC2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010466.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VSG3, Q04002

UniProtKB/TrEMBL

A6ZYD9, N1P8C5

Conserved Domains (2) summary

pfam12765
Location:721 → 762

Cohesin_HEAT; HEAT repeat associated with sister chromatid cohesion

pfam12830
Location:1207 → 1384

Nipped-B_C; Sister chromatid cohesion C-terminus