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    BUD6 Bud6p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851029, updated on 9-Dec-2024

    Summary

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    Official Symbol
    BUD6
    Official Full Name
    Bud6p
    Primary source
    SGD:S000004311
    Locus tag
    YLR319C
    See related
    AllianceGenome:SGD:S000004311; FungiDB:YLR319C; VEuPathDB:YLR319C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    AIP3
    Summary
    Enables actin binding activity; cytoskeletal regulatory protein binding activity; and enzyme activator activity. Involved in several processes, including bipolar cellular bud site selection; cytoskeleton organization; and positive regulation of supramolecular fiber organization. Located in several cellular components, including cellular bud tip; incipient cellular bud site; and mating projection tip. Part of polarisome. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See BUD6 in Genome Data Viewer
    Location:
    chromosome: XII
    Exon count:
    1
    Sequence:
    Chromosome: XII; NC_001144.5 (769318..771684, complement)

    Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Nkp2p Neighboring gene Tad3p Neighboring gene telomerase reverse transcriptase Neighboring gene Mms22p Neighboring gene Sfh1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001144.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Fission yeast Aip3p (spAip3p) is required for an alternative actin-directed polarity program. Jin H, et al. Mol Biol Cell, 2001 May. PMID 11359921, Free PMC Article
    2. Kar9p-independent microtubule capture at Bud6p cortical sites primes spindle polarity before bud emergence in Saccharomyces cerevisiae. Segal M, et al. Mol Biol Cell, 2002 Dec. PMID 12475941, Free PMC Article
    3. Septin-dependent compartmentalization of the endoplasmic reticulum during yeast polarized growth. Luedeke C, et al. J Cell Biol, 2005 Jun 20. PMID 15967812, Free PMC Article
    4. Aip3p/Bud6p, a yeast actin-interacting protein that is involved in morphogenesis and the selection of bipolar budding sites. Amberg DC, et al. Mol Biol Cell, 1997 Apr. PMID 9247651, Free PMC Article
    5. Severing of F-actin by yeast cofilin is pH-independent. Pavlov D, et al. Cell Motil Cytoskeleton, 2006 Sep. PMID 16847879, Free PMC Article

    See all (115) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A teamwork promotion of formin-mediated actin nucleation by Bud6 and Aip5 in Saccharomyces cerevisiae.
      Title: A teamwork promotion of formin-mediated actin nucleation by Bud6 and Aip5 in Saccharomyces cerevisiae.
    2. Structure of a Bud6/Actin complex reveals a novel wh2-like actin monomer recruitment motif.
      Title: Structure of a Bud6/Actin Complex Reveals a Novel WH2-like Actin Monomer Recruitment Motif.
    3. Yor304c-a/Bil1 colocalizes with Bud6 and functions in the Bnr1 pathway for actin assembly
      Title: Ligand-induced activation of a formin-NPF pair leads to collaborative actin nucleation.
    4. model in which a Bni1/Bud6 complex with a 2:4 subunit stoichiometry assembles a nucleation seed with Bud6 coordinating up to four actin subunits
      Title: Structure of the formin-interaction domain of the actin nucleation-promoting factor Bud6.
    5. Bud6p and Kip3p play antagonistic roles in controlling the length of microtubules contacting the bud.
      Title: Mechanism for astral microtubule capture by cortical Bud6p priming spindle polarity in S. cerevisiae.
    6. Bud6 promotes actin filament nucleation by recruiting actin monomers to Bni1
      Title: Mechanism and cellular function of Bud6 as an actin nucleation-promoting factor.
    7. BNI1 and BNR1 contribute to Bud6p-dependent microtubule-cortex interactions by controlling Bud6p localisation.
      Title: Dissecting the involvement of formins in Bud6p-mediated cortical capture of microtubules in S. cerevisiae.
    8. Bni1 and Bnr1 are regulated by Bud6
      Title: Differential activities and regulation of Saccharomyces cerevisiae formin proteins Bni1 and Bnr1 by Bud6.
    9. Bud6p, through its localization at both spindle pole bodies and at the mother-bud neck, provides a regulatory mechanism to prevent excessive oscillations of preanaphase nuclei, thus reducing the likelihood of mitotic delays and nuclear missegregation.
      Title: Stable preanaphase spindle positioning requires Bud6p and an apparent interaction between the spindle pole bodies and the neck.
    10. Bud6p has a role in MT capture at the cell cortex, independent of Kar9p-mediated MT delivery along actin cables
      Title: Differential contribution of Bud6p and Kar9p to microtubule capture and spindle orientation in S. cerevisiae.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables actin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cytoskeletal regulatory protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cytoskeletal regulatory protein binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cytoskeletal regulatory protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables enzyme activator activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in actin filament bundle assembly IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in actin filament bundle assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in astral microtubule organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in bipolar cellular bud site selection IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in budding cell apical bud growth IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in budding cell apical bud growth IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular hyperosmotic response IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in cellular hyperosmotic response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in establishment of cell polarity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in establishment of cell polarity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of actin nucleation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of actin nucleation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in positive regulation of formin-nucleated actin cable assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of formin-nucleated actin cable assembly IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in pseudohyphal growth IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of actin cytoskeleton organization IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in regulation of actin cytoskeleton organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of protein localization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cell tip IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cellular bud neck IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cellular bud tip IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in incipient cellular bud site IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mating projection tip HDA PubMed 
    located_in mating projection tip IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of polarisome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in prospore membrane HDA PubMed 
    located_in secretory vesicle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in spindle pole body IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Bud6p
    NP_013423.1
    • Actin- and formin-interacting protein; nucleation-promoting factor (NPF) for Bni1p and Bnr1p during actin cable assembly and organization; enhances Bnr1p-mediated actin nucleation, alleviating Hof1p inhibition through competitive binding; interacts with Bni1p via a C-terminal triple helical coiled-coil domain; role in polarized cell growth; isolated as a bipolar budding mutant; localizes to the presumptive bud site, small bud tips, the bud neck and secretory vesicles; potential Cdc28p substrate

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001144.5 Reference assembly

      Range
      769318..771684 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182208.1 → NP_013423.1  TPA: Bud6p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013423.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VYW2, P41697
      UniProtKB/TrEMBL
      A7A1K3, C7GUB4, G2WJE1, N1P006
      Conserved Domains (1) summary
      smart00806
      Location:365 → 776
      AIP3; Actin interacting protein 3
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P41697.1

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