Galphai G protein alpha subunit i [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Galphaiprovided by FlyBase
Official Full Name: G protein alpha subunit iprovided by FlyBase
Primary source: FLYBASE:FBgn0001104
Locus tag: Dmel_CG10060
See related: AllianceGenome:FB:FBgn0001104
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG10060; DGalpha1; DG[[alpha1]]; Dmel\CG10060; G(alphai); G-alpha-65A; G-ia65A; G-ialpha65a; G-ialpha65A; G-oalpha65A; Gai; Galpha i; Galpha65A; galphai; GalphaI; Galphai/o; Galphaiota65A; Galpha[[i65A]]; Galpha[[i]]; Galpha[[i]]65A; Gi; Gi-alpha; Gialpha; GNAI1/2/3; G[[alphai]]; G[[i]]; G[[i]]alpha; i
Summary: Enables DNA binding activity. Involved in several processes, including asymmetric protein localization involved in cell fate determination; cytoskeleton organization; and nervous system development. Located in apical cortex. Is expressed in several structures, including CNS glial cell; ganglia; germline cell; organism subdivision; and photoreceptor neurons. Human ortholog(s) of this gene implicated in achromatopsia 4; color blindness; congenital stationary night blindness 1G; congenital stationary night blindness autosomal dominant 3; and night blindness. Orthologous to several human genes including GNAI1 (G protein subunit alpha i1); GNAI2 (G protein subunit alpha i2); and GNAI3 (G protein subunit alpha i3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 65D5-65D5; 3-18 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (6972750..6980044, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (6965850..6973144, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Galphai is not necessary for the control of the anteroposterior orientation of the mitotic spindle during pl cell division downstream of Frizzled signalling, but they are required for maintainance of the spindle within the plane of the epithelium
Title: Drosophila Ric-8 regulates Galphai cortical localization to promote Galphai-dependent planar orientation of the mitotic spindle during asymmetric cell division.
Pins can mediate asymmetric spindle geometry.[PINs]. Comparisons of the phenotypes of Galphai, Ric-8, Gbeta13Fsingle and Ric-8;Gbeta13F double loss-of-function mutants indicate that, in NBs, Ric-8 positively regulates Gai activity.
Title: Ric-8 controls Drosophila neural progenitor asymmetric division by regulating heterotrimeric G proteins.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables G protein-coupled receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables G-protein beta/gamma-subunit complex binding	IBA Inferred from Biological aspect of Ancestor more info
enables G-protein beta/gamma-subunit complex binding	IEA Inferred from Electronic Annotation more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in adenylate cyclase-modulating G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in adenylate cyclase-modulating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in asymmetric cell division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in asymmetric neuroblast division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in asymmetric protein localization involved in cell fate determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon ensheathment in central nervous system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in calcium-mediated signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cortical actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of glial blood-brain barrier	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in septate junction assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ventral cord development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in apical cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	TAS Traceable Author Statement more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
part_of heterotrimeric G-protein complex	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	HDA more info	PubMed

General protein information

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Preferred Names: G protein alpha subunit i

Names: CG10060-PA; G protein I a-subunit 65A; G protein alpha i subunit; G protein alpha o subunit 65A; G protein alpha subunit; G protein alpha-subunit; G protein alphai subunit; G protein alphai subunit 65A; G protein alphao subunit 65A; Galphai-PA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

6972750..6980044 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_058154.5 → NP_477502.1 G protein alpha subunit i [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477502.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P20353, Q9VS04

Related

FBpp0076643

Conserved Domains (2) summary

cd00066
Location:35 → 349

G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)

smart00275
Location:14 → 353

G_alpha; G protein alpha subunit