Nbr Nibbler [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Nbrprovided by FlyBase
Official Full Name: Nibblerprovided by FlyBase
Primary source: FLYBASE:FBgn0032924
Locus tag: Dmel_CG9247
See related: AllianceGenome:FB:FBgn0032924
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG9247; Dmel\CG9247; nbr
Summary: Enables 3'-5'-exoribonuclease activity involved in mature miRNA 3'-end processing. Involved in regulatory ncRNA-mediated gene silencing and retrotransposon silencing. Part of ribonucleoprotein complex. Is active in P granule. Is expressed in spermatozoon. Orthologous to human EXD3 (exonuclease 3'-5' domain containing 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 39A6-39A7; 2-54 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (21160636..21163083, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (21160636..21163083, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Endogenous piRNA-guided slicing triggers responder and trailer piRNA production from viral RNA in Aedes aegypti mosquitoes.
Title: Endogenous piRNA-guided slicing triggers responder and trailer piRNA production from viral RNA in Aedes aegypti mosquitoes.
Nbr is expressed only during a short time of oogenesis and appears ubiquitously localized within oocytes. Nbr was is not enriched in the nuage where it was shown to be involved in piwi-mediated mechanisms.
Title: Drosophila exoribonuclease nibbler is a tumor suppressor, acts within the RNA(i) machinery and is not enriched in the nuage during early oogenesis.
antagonistic roles of Nbr and Hen1 define a mechanism to modulate piRNA 3' ends.
Title: Antagonistic roles of Nibbler and Hen1 in modulating piRNA 3' ends in Drosophila.
indicate a role of Nibbler on age-associated processes and to modulate length of multiple classes of small RNAs including miRNAs and piRNAs in Drosophila
Title: The exonuclease Nibbler regulates age-associated traits and modulates piRNA length in Drosophila.
The study provides a molecular explanation for the previously reported heterogeneity of miRNA 3' ends and proposes a model in which Nibbler (CG9247)converts miRNAs into isoforms that are compatible with the preferred length of Ago1-bound small RNAs.
Title: The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs bound to Drosophila Argonaute1.
An RNA interference screen identified a putative 3'-5' exoribonuclease CG9247/nibbler essential for the generation of smaller isoforms of the microRNA miR-34. Nibbler interacts with Argonaute1 and processes miR-34 within the RNA-induced silencing complex.
Title: The exoribonuclease Nibbler controls 3' end processing of microRNAs in Drosophila.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables 3'-5' exonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3'-5' exonuclease activity	IEA Inferred from Electronic Annotation more info
enables 3'-5'-RNA exonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables 3'-5'-exoribonuclease activity involved in mature miRNA 3'-end processing	IMP Inferred from Mutant Phenotype more info	PubMed
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in miRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleobase-containing compound metabolic process	IEA Inferred from Electronic Annotation more info
involved_in piRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pre-miRNA 3'-end processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in primary piRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in secondary piRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transposable element silencing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in P granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
part_of ribonucleoprotein complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: nibbler

Names: CG9247-PA; Nbr-PA

NP_610094.1

EC 3.1.13.-

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

21160636..21163083 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_136250.4 → NP_610094.1 nibbler [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_610094.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8MSZ9, Q9VIF1

UniProtKB/TrEMBL

G7H828

Related

FBpp0081027

Conserved Domains (1) summary

cd06146
Location:413 → 599

mut-7_like_exo; DEDDy 3'-5' exonuclease domain of Caenorhabditis elegans mut-7 and similar proteins