scaf scarface [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: scafprovided by FlyBase
Official Full Name: scarfaceprovided by FlyBase
Primary source: FLYBASE:FBgn0033033
Locus tag: Dmel_CG11066
See related: AllianceGenome:FB:FBgn0033033
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG11066; CG15900; cSPH142; Dmel\CG11066; Scaf; scarf; SPH142
Summary: Involved in embryonic development via the syncytial blastoderm; establishment or maintenance of epithelial cell apical/basal polarity; and imaginal disc morphogenesis. Located in cytoplasm and extracellular space. Colocalizes with apical plasma membrane and endosome. Is expressed in several structures, including adult head; dorsal epidermis primordium; embryonic head epidermis; embryonic ventral epidermis; and epithelium. [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 41F6-41F8; 2-55 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (5676750..5691723, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (1564255..1579228, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Serine Protease Homolog, Scarface, Is Sensitive to Nutrient Availability and Modulates the Development of the Drosophila Blood-Brain Barrier.
Title: The Serine Protease Homolog, Scarface, Is Sensitive to Nutrient Availability and Modulates the Development of the Drosophila Blood-Brain Barrier.
scaf promotes feeding in response to the presence of sugar in the food, therefore providing a mechanism for the regulation of hedonic feeding.
Title: Sugar Promotes Feeding in Flies via the Serine Protease Homolog scarface.
Here we uncover a cell non-autonomous requirement for the Epidermal growth factor receptor (Egfr) pathway in the lateral epidermis for sustained dpp expression in the LE. Specifically, we demonstrate that Egfr pathway activity in the lateral epidermis prevents expression of the gene scarface (scaf), encoding a secreted antagonist of JNK signaling
Title: Novel interplay between JNK and Egfr signaling in Drosophila dorsal closure.
Scarface has a key role in regulating the polarized localization of laminin A in this developmental context.
Title: Scarface, a secreted serine protease-like protein, regulates polarized localization of laminin A at the basement membrane of the Drosophila embryo.
Results identify the serine protease homologue-encoding gene scaf as a new transcriptional target of JNK signalling and reveal the first secreted regulator of the JNK pathway acting in a negative-feedback loop during epithelial morphogenesis.
Title: The Drosophila serine protease homologue Scarface regulates JNK signalling in a negative-feedback loop during epithelial morphogenesis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

mobile_element

Loc: c5678202-5678146 mobile_element_type = transposon:baggins{}5698
Loc: c5679027-5678594 mobile_element_type = transposon:INE-1{}5088

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
NOT enables peptidase activity	IKR Inferred from Key Residues more info
NOT enables serine-type endopeptidase activity	IKR Inferred from Key Residues more info	PubMed

Process	Evidence Code	Pubs
involved_in dorsal closure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dorsal closure, elongation of leading edge cells	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of epithelial cell apical/basal polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ-band shortening	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in imaginal disc-derived male genitalia morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in innate immune response	IBA Inferred from Biological aspect of Ancestor more info
involved_in notum morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in regulation of developmental process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
colocalizes_with apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with early endosome	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IDA Inferred from Direct Assay more info	PubMed
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with late endosome	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with recycling endosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: scarface

Names: CG11066-PA; CG11066-PB; scaf-PA; scaf-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

5676750..5691723 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_136336.3 → NP_610180.1 scarface, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_610180.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q7K5M0

Related

FBpp0085396

Conserved Domains (2) summary

cd00190
Location:428 → 616

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

cl28087
Location:198 → 334

FtsK; DNA segregation ATPase FtsK/SpoIIIE and related proteins [Cell cycle control, cell division, chromosome partitioning]
NM_165441.2 → NP_724424.1 scarface, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724424.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q7K5M0

Related

FBpp0085397

Conserved Domains (2) summary

cd00190
Location:428 → 616

Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...

cl28087
Location:198 → 334

FtsK; DNA segregation ATPase FtsK/SpoIIIE and related proteins [Cell cycle control, cell division, chromosome partitioning]