TYW1 putative tRNA 4-demethylwyosine synthase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TYW1
Official Full Name: putative tRNA 4-demethylwyosine synthase
Primary source: SGD:S000006128
Locus tag: YPL207W
See related: AllianceGenome:SGD:S000006128; FungiDB:YPL207W; VEuPathDB:YPL207W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables S-adenosyl-L-methionine binding activity. Involved in wybutosine biosynthetic process. Located in endoplasmic reticulum. Orthologous to human TYW1 (tRNA-yW synthesizing protein 1 homolog) and TYW1B (tRNA-yW synthesizing protein 1 homolog B). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See TYW1 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (159909..162341)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Eukaryotic TYW1 Is a Radical SAM Flavoenzyme.
Title: Eukaryotic TYW1 Is a Radical SAM Flavoenzyme.
the Yap5-mediated induction of TYW1 provides protection from high iron toxicity by the consumption of free cytosolic iron through the formation of protein-bound iron-sulfur clusters
Title: Yap5 protein-regulated transcription of the TYW1 gene protects yeast from high iron toxicity.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 4 iron, 4 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables FMN binding	IEA Inferred from Electronic Annotation more info
enables S-adenosyl-L-methionine binding	IDA Inferred from Direct Assay more info	PubMed
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables lyase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables tRNA-4-demethylwyosine synthase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in tRNA processing	IEA Inferred from Electronic Annotation more info
involved_in wybutosine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in wybutosine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in wybutosine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: putative tRNA 4-demethylwyosine synthase

NP_015117.1

EC 4.1.3.44

Iron-sulfur protein required for synthesis of wybutosine modified tRNA; wybutosine is a modified guanosine found at 3'-position adjacent to anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

159909..162341

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184021.1 → NP_015117.1 TPA: putative tRNA 4-demethylwyosine synthase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015117.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W3G3, Q08960

UniProtKB/TrEMBL

A6ZW48, B3LKM3, C8ZIH2

Conserved Domains (2) summary

COG0731
Location:445 → 762

Tyw1; Wyosine [tRNA(Phe)-imidazoG37] synthetase, radical SAM superfamily [Translation, ribosomal structure and biogenesis]

pfam00258
Location:207 → 354

Flavodoxin_1