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    SPP2 Spp2p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 854319, updated on 9-Dec-2024

    Summary

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    Official Symbol
    SPP2
    Official Full Name
    Spp2p
    Primary source
    SGD:S000005674
    Locus tag
    YOR148C
    See related
    AllianceGenome:SGD:S000005674; FungiDB:YOR148C; VEuPathDB:YOR148C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables ATPase activator activity. Involved in mRNA splicing, via spliceosome. Part of spliceosomal complex. Orthologous to human GPKOW (G-patch domain and KOW motifs). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See SPP2 in Genome Data Viewer
    Location:
    chromosome: XV
    Exon count:
    1
    Sequence:
    Chromosome: XV; NC_001147.6 (608640..609197, complement)

    Chromosome XV - NC_001147.6Genomic Context describing neighboring genes Neighboring gene Pno1p Neighboring gene Mdm32p Neighboring gene glycosylphosphatidylinositol-alpha 1,2 mannosyltransferase Neighboring gene mitochondrial 54S ribosomal protein YmL23

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001147.6

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The final stages of spliceosome maturation require Spp2p that can interact with the DEAH box protein Prp2p and promote step 1 of splicing. Roy J, et al. RNA, 1995 Jun. PMID 7493316, Free PMC Article
    2. Mechanism of spliceosome remodeling by the ATPase/helicase Prp2 and its coactivator Spp2. Bai R, et al. Science, 2021 Jan 8. PMID 33243853
    3. Structural analysis of the intrinsically disordered splicing factor Spp2 and its binding to the DEAH-box ATPase Prp2. Hamann F, et al. Proc Natl Acad Sci U S A, 2020 Feb 11. PMID 31974312, Free PMC Article
    4. Interaction between a G-patch protein and a spliceosomal DEXD/H-box ATPase that is critical for splicing. Silverman EJ, et al. Mol Cell Biol, 2004 Dec. PMID 15542821, Free PMC Article
    5. Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing. Krishnan R, et al. Nat Struct Mol Biol, 2013 Dec. PMID 24240612, Free PMC Article

    See all (26) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Mechanism of spliceosome remodeling by the ATPase/helicase Prp2 and its coactivator Spp2.
      Title: Mechanism of spliceosome remodeling by the ATPase/helicase Prp2 and its coactivator Spp2.
    2. The structural analysis unveils the role of conserved residues of the G-patch in the dynamic interaction mode of Spp2 with Prp2, which is vital to maintain the binding during the Prp2 domain movements needed for RNA translocation.
      Title: Structural analysis of the intrinsically disordered splicing factor Spp2 and its binding to the DEAH-box ATPase Prp2.
    3. Spp2 is not required to recruit Prp2 to its bona fide binding site in the B(act) spliceosome.
      Title: The G-patch protein Spp2 couples the spliceosome-stimulated ATPase activity of the DEAH-box protein Prp2 to catalytic activation of the spliceosome.
    4. there is a mechanism for Prp2 specificity mediated through a unique interaction with Spp2
      Title: Interaction between a G-patch protein and a spliceosomal DEXD/H-box ATPase that is critical for splicing.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATPase activator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA splicing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA splicing, via spliceosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA splicing, via spliceosome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of spliceosomal complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of spliceosomal complex IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Spp2p
    NP_014791.1
    • Essential protein that promotes the first step of splicing; required for the final stages of spliceosome maturation and activation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction; stimulates Prp2p ATPase activity

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001147.6 Reference assembly

      Range
      608640..609197 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001183567.1 → NP_014791.1  TPA: Spp2p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_014791.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W2K5, Q02521, Q12353
      UniProtKB/TrEMBL
      C7GN76, G2WN20, N1NYH6
      Conserved Domains (1) summary
      pfam12656
      Location:93 → 151
      G-patch_2; G-patch domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q02521.1

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