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    SRS2 DNA helicase SRS2 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853353, updated on 9-Dec-2024

    Summary

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    Official Symbol
    SRS2
    Official Full Name
    DNA helicase SRS2
    Primary source
    SGD:S000003628
    Locus tag
    YJL092W
    See related
    AllianceGenome:SGD:S000003628; FungiDB:YJL092W; VEuPathDB:YJL092W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    HPR5
    Summary
    Enables 3'-5' DNA helicase activity and enzyme activator activity. Involved in several processes, including DNA metabolic process; DNA recombinase disassembly; and negative regulation of double-strand break repair via homologous recombination. Located in nucleus. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See SRS2 in Genome Data Viewer
    Location:
    chromosome: X
    Exon count:
    1
    Sequence:
    Chromosome: X; NC_001142.9 (257423..260947)

    Chromosome X - NC_001142.9Genomic Context describing neighboring genes Neighboring gene Kha1p Neighboring gene Tok1p Neighboring gene glucosaminyl-phosphotidylinositol O-acyltransferase Neighboring gene protein kinase activating protein DPB11

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001142.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Purification and characterization of the SRS2 DNA helicase of the yeast Saccharomyces cerevisiae. Rong L, et al. J Biol Chem, 1993 Jan 15. PMID 8419328
    2. The Srs2 helicase prevents recombination by disrupting Rad51 nucleoprotein filaments. Veaute X, et al. Nature, 2003 May 15. PMID 12748645
    3. ATPase and DNA helicase activities of the Saccharomyces cerevisiae anti-recombinase Srs2. Van Komen S, et al. J Biol Chem, 2003 Nov 7. PMID 12966095
    4. Overcoming natural replication barriers: differential helicase requirements. Anand RP, et al. Nucleic Acids Res, 2012 Feb. PMID 21984413, Free PMC Article
    5. Yeast Srs2 Helicase Promotes Redistribution of Single-Stranded DNA-Bound RPA and Rad52 in Homologous Recombination Regulation. De Tullio L, et al. Cell Rep, 2017 Oct 17. PMID 29045827, Free PMC Article

    See all (289) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases.
      Title: Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases.
    2. Genetic Dissection of Budding Yeast PCNA Mutations Responsible for the Regulated Recruitment of Srs2 Helicase.
      Title: Genetic Dissection of Budding Yeast PCNA Mutations Responsible for the Regulated Recruitment of Srs2 Helicase.
    3. Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments.
      Title: Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments.
    4. Prevention of unwanted recombination at damaged replication forks.
      Title: Prevention of unwanted recombination at damaged replication forks.
    5. Srs2 C-terminal Rad51 interaction domain is essential for crossover control in S. cerevisiae.
      Title: Role of the Srs2-Rad51 Interaction Domain in Crossover Control in Saccharomyces cerevisiae.
    6. DNA helicase domain of Saccharomyces cerevisiae yeast Srs2 protein is needed to deal with DNA damage and assist in DNA replication during vegetative growth and in meiosis. The interactions of Srs2 with Rad51 Recombinase and proliferating cell nuclear Antigen PCNA are dispensable for the main role of Srs2.
      Title: The Main Role of Srs2 in DNA Repair Depends on Its Helicase Activity, Rather than on Its Interactions with PCNA or Rad51.
    7. Two main activities of Srs2, strippase and helicase, counteract toxic joint intermediates formed during break-induced replication by preventing their formation and promoting their disruption, respectively.
      Title: Break-induced replication promotes formation of lethal joint molecules dissolved by Srs2.
    8. Srs2 rapidly disrupts small heteroduplex DNA joints bound to Rad51 filaments.
      Title: Dissociation of Rad51 Presynaptic Complexes and Heteroduplex DNA Joints by Tandem Assemblies of Srs2.
    9. These data establish a biochemical mechanism for the role of Srs2 in crossover suppression by promoting synthesis-dependent strand annealing through disruption of DNA polymerase delta-extended D-loops.
      Title: Srs2 promotes synthesis-dependent strand annealing by disrupting DNA polymerase δ-extending D-loops.
    10. Srs2 interaction with PCNA allows the helicase activity to unwind fork-blocking CAG/CTG hairpin structures to prevent DNA breaks during DNA replication. Independently of PCNA binding, Srs2 also displaces Rad51 from nascent strands to prevent recombination-dependent repeat expansions and contractions.
      Title: Differential requirement of Srs2 helicase and Rad51 displacement activities in replication of hairpin-forming CAG/CTG repeats.
    Submit: New GeneRIF Correction See all GeneRIFs (43)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables 3'-5' DNA helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 3'-5' DNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H2AZ histone chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent H3-H4 histone complex chaperone activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA clamp loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables chromatin extrusion motor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cohesin loader activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables double-stranded DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables enzyme activator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables forked DNA-dependent helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables four-way junction helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables single-stranded 3'-5' DNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA duplex unwinding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA protection IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in DNA recombinase disassembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA recombinase disassembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in chromatin looping IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin remodeling IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in double-strand break repair via nonhomologous end joining IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in meiosis I IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of DNA recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of double-strand break repair via homologous recombination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in recombinational repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    DNA helicase SRS2
    NP_012443.1
    • DNA helicase and DNA-dependent ATPase; role in DNA repair and checkpoint recovery, in the proper timing of commitment to meiotic recombination and the Meiosis I to II transition; blocks trinucleotide repeat expansion; affects genome stability; disassembles Rad51p nucleoprotein filaments during meiotic recombination; stimulates Mus81p-Mms4p endonuclease activity independent of catalytic activity; ATPase and ssDNA translocating motor activities inhibited by Dmc1p; functional homolog of human RTEL1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001142.9 Reference assembly

      Range
      257423..260947
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001181525.1 → NP_012443.1  TPA: DNA helicase SRS2 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012443.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VW92, P12954
      UniProtKB/TrEMBL
      B3LQ21, C7GR22, C8ZBC1, N1P8F3
      Conserved Domains (1) summary
      COG0210
      Location:13 → 750
      UvrD; Superfamily I DNA or RNA helicase [Replication, recombination and repair]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P12954.2

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