RCF1 Rcf1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RCF1
Official Full Name: Rcf1p
Primary source: SGD:S000004492
Locus tag: YML030W
See related: AllianceGenome:SGD:S000004492; FungiDB:YML030W; VEuPathDB:YML030W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: AIM31
Summary: Involved in mitochondrial respirasome assembly and regulation of proton transport. Acts upstream of or within mitochondrial cytochrome c oxidase assembly. Located in mitochondrial inner membrane. Part of respiratory chain complex. Orthologous to several human genes including HIGD2A (HIG1 hypoxia inducible domain family member 2A). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RCF1 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (216435..216914)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Using yeast as a model organism, we have shown previously that its two HIGD1 proteins, Rcf1 and Rcf2, are required for the generation and maintenance of a normal membrane potential (DeltaPsi) across the inner mitochondrial membrane (IMM). We postulated that the lower DeltaPsi observed in the absence of the HIGD1 proteins may be due to decreased proton pumping by complex IV (CIV) or enhanced leak of protons across the IMM
Title: Hypoxia-inducible gene domain 1 proteins in yeast mitochondria protect against proton leak through complex IV.
the absence of Rcf1 and Rcf2 proteins on the CIV complex are distinct in terms of CIV assembly/accumulation and additive in terms of the ability of CIV to generate PMF. Thus, the combined absence of Rcf1 and Rcf2 alters both CIV physiology and assembly.
Title: The yeast mitochondrial proteins Rcf1 and Rcf2 support the enzymology of the cytochrome c oxidase complex and generation of the proton motive force.
The turnover of CytcO was less blocked in rcf1Delta than in the wild-type mitochondria, suggesting that Rcf1 modulates the structure of the catalytic site.
Title: Modulation of O(2) reduction in Saccharomyces cerevisiae mitochondria.
Data suggest the regulatory mechanism of the respiratory supercomplex factors 1 and 2 (Rcf1 and Rcf2) in the context of supramolecular interactions between cyt. bc1 and CytcO.
Title: Regulatory role of the respiratory supercomplex factors in Saccharomyces cerevisiae.
mutation of conserved residues within the Rcf1 QRRQ motif alters the interactions between Rcf1 and partner proteins and results in the destabilization of complex IV and alteration of its enzymatic properties
Title: Mutational Analysis of the QRRQ Motif in the Yeast Hig1 Type 2 Protein Rcf1 Reveals a Regulatory Role for the Cytochrome c Oxidase Complex.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in mitochondrial cytochrome c oxidase assembly	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within mitochondrial cytochrome c oxidase assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial respirasome assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrial respirasome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of proton transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
part_of respiratory chain complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Rcf1p

NP_013682.1

Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast