SPT15 TATA-binding protein [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SPT15
Official Full Name: TATA-binding protein
Primary source: SGD:S000000950
Locus tag: YER148W
See related: AllianceGenome:SGD:S000000950; FungiDB:YER148W; VEuPathDB:YER148W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: BTF1; TBP1
Summary: Enables several functions, including DNA binding activity; RNA polymerase I general transcription initiation factor binding activity; and RNA polymerase II-specific DNA-binding transcription factor binding activity. Involved in DNA-templated transcription. Located in nucleus. Part of transcription factor TFIID complex and transcription factor TFIIIB complex. Used to study spinocerebellar ataxia type 17. Human ortholog(s) of this gene implicated in late onset Parkinson's disease; schizophrenia; spinocerebellar ataxia type 17; and type 1 diabetes mellitus. Orthologous to several human genes including TBPL2 (TATA-box binding protein like 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SPT15 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (465303..466025)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural convergence endows nuclear transport receptor Kap114p with a transcriptional repressor function toward TATA-binding protein.
Title: Structural convergence endows nuclear transport receptor Kap114p with a transcriptional repressor function toward TATA-binding protein.
Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.
Title: Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.
Architecture of the multi-functional SAGA complex and the molecular mechanism of holding TBP.
Title: Architecture of the multi-functional SAGA complex and the molecular mechanism of holding TBP.
structure of yeast SAGA with bound TBP
Title: Structure of SAGA and mechanism of TBP deposition on gene promoters.
Transcription of nearly all yeast RNA polymerase II-transcribed genes is dependent on transcription factor TFIID.
Title: SAGA Is a General Cofactor for RNA Polymerase II Transcription.
Transcription of nearly all yeast RNA polymerase II-transcribed genes is dependent on transcription factor TFIID.
Title: Transcription of Nearly All Yeast RNA Polymerase II-Transcribed Genes Is Dependent on Transcription Factor TFIID.
this study identified two SPT15 alleles, nine gene deletions and low concentration of amino acids in the medium that confer enhanced tolerance to acetic acid.
Title: Tolerance to acetic acid is improved by mutations of the TATA-binding protein gene.
SPT15 confers enhanced tolerance to hyperosmotic stress.
Title: Mutations of the TATA-binding protein confer enhanced tolerance to hyperosmotic stress in Saccharomyces cerevisiae.
The action of Mot1 is required to clear TBP from intrinsically preferred (TATA-containing) binding sites, ensuring sufficient soluble TBP to bind intrinsically disfavored (TATA-less) sites.
Title: Mot1 redistributes TBP from TATA-containing to TATA-less promoters.
In the absence of ATP, ternary complex stability arises from both Mot1-DNA and Mot1-TBP interactions.
Title: Two-step mechanism for modifier of transcription 1 (Mot1) enzyme-catalyzed displacement of TATA-binding protein (TBP) from DNA.

Submit: New GeneRIF Correction See all GeneRIFs (23)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA binding, bending	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase I general transcription initiation factor binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase I general transcription initiation factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II core promoter sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II general transcription initiation factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase III transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA-templated transcription initiation	IEA Inferred from Electronic Annotation more info
involved_in RNA polymerase I preinitiation complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in RNA polymerase II preinitiation complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in RNA polymerase III preinitiation complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleolar large rRNA transcription by RNA polymerase I	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of transcription factor TFIID complex	IDA Inferred from Direct Assay more info	PubMed
part_of transcription factor TFIIIB complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: TATA-binding protein

NP_011075.3

TATA-binding protein (TBP); general transcription factor that interacts with other factors to form the preinitiation complex at promoters; essential for viability, highly conserved; yeast gene can complement mutations in human homolog TBP