Mras muscle and microspikes RAS [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mrasprovided by MGI
Official Full Name: muscle and microspikes RASprovided by MGI
Primary source: MGI:MGI:1100856
See related: Ensembl:ENSMUSG00000032470 AllianceGenome:MGI:1100856
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 2900078C09Rik
Summary: Predicted to enable GTP-dependent protein binding activity; GTPase activity; and guanyl ribonucleotide binding activity. Acts upstream of or within cellular response to leukemia inhibitory factor. Predicted to be part of protein phosphatase type 1 complex. Predicted to be active in plasma membrane. Is expressed in several structures, including brain; branchial arch; future spinal cord; limb bud; and liver. Human ortholog(s) of this gene implicated in Noonan syndrome 11. Orthologous to human MRAS (muscle RAS oncogene homolog). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in cortex adult (RPKM 34.0), ovary adult (RPKM 30.7) and 21 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 E3.3; 9 51.63 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (99267473..99319505, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (99385420..99437700, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

This study demonstrated that M-Ras is recruited to the membrane of phagocytic cups and contributes to the regulation of phagosome formation in macrophages.
Title: Transient recruitment of M-Ras GTPase to phagocytic cups in RAW264 macrophages during FcγR-mediated phagocytosis.
Expression of the muscarinic M2 and M3 receptors that mediate the cholinergic contractile stimuli of the detrusor muscle was dysregulated in both Mras-/- males and females, although only males exhibited a urinary phenotype.
Title: Urinary Retention, Incontinence, and Dysregulation of Muscarinic Receptors in Male Mice Lacking Mras.
Overexpression of MRAS in mouse embryonic stem cells leads to different cellular phenotypes.
Title: MRAS GTPase is a novel stemness marker that impacts mouse embryonic stem cell plasticity and Xenopus embryonic cell fate.
succeed in solving two crystal structures corresponding to state 1 and state 2 from a single Ras polypeptide, M-RasD41E, carrying an H-Ras-type substitution in residue 41
Title: Critical roles of interactions among switch I-preceding residues and between switch II and its neighboring alpha-helix in conformational dynamics of the GTP-bound Ras family small GTPases.
X-ray crystal structure analyses of a series of mutant H-Ras and M-Ras in complex with guanosine 5'-(beta,gamma-imido)triphosphate (GppNHp), representing various intermediate states of the transition, were determined.
Title: Structural basis for conformational dynamics of GTP-bound Ras protein.
These results imply that M-Ras, induced and activated by BMP-2 signaling, participates in the osteoblastic determination, differentiation, and transdifferentiation under p38 MAPK and JNK regulation
Title: M-Ras is activated by bone morphogenetic protein-2 and participates in osteoblastic determination, differentiation, and transdifferentiation.
Conserved expression of Mras was observed in the nervous system both in mice and in ascidians.
Title: M-Ras evolved independently of R-Ras and its neural function is conserved between mammalian and ascidian, which lacks classical Ras.
These findings proved a crucial role of the cross-talk between two Ras-family GTPases M-Ras and Rap1, mediated by RA-GEF-2, in adhesion signaling.
Title: The M-Ras-RA-GEF-2-Rap1 pathway mediates tumor necrosis factor-alpha dependent regulation of integrin activation in splenocytes.
Expression of activated mutants of M-Ras was sufficient for oncogenic transformation of a murine mammary epithelial cell line
Title: Expression of activated M-Ras in a murine mammary epithelial cell line induces epithelial-mesenchymal transition and tumorigenesis.
Data describe the crystal structure of M-Ras in the GDP-bound and guanosine 5'-(beta,gamma-imido)triphosphate (Gpp(NH)p)-bound forms.
Title: Crystal structure of M-Ras reveals a GTP-bound "off" state conformation of Ras family small GTPases.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables G protein activity	IEA Inferred from Electronic Annotation more info
enables GDP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTP-dependent protein binding	IEA Inferred from Electronic Annotation more info
enables GTP-dependent protein binding	ISO Inferred from Sequence Orthology more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in Ras protein signal transduction	IEA Inferred from Electronic Annotation more info
involved_in Ras protein signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to leukemia inhibitory factor	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
part_of protein phosphatase type 1 complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ras-related protein M-Ras

Names: X-Ras; ras-related protein R-Ras3

NP_032650.1

EC 3.6.5.2

XP_006510888.1

EC 3.6.5.2

XP_030099940.1

EC 3.6.5.2

XP_036010535.1

EC 3.6.5.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008624.3 → NP_032650.1 ras-related protein M-Ras

See identical proteins and their annotated locations for NP_032650.1

Status: VALIDATED

Source sequence(s)

AB004879, AC157994, AK050469, BC023079, CJ162751, CX122916

Consensus CDS

CCDS23433.1

UniProtKB/Swiss-Prot

O08989

UniProtKB/TrEMBL

Q3TPX5

Related

ENSMUSP00000035045.9, ENSMUST00000035045.15

Conserved Domains (1) summary

cd04145
Location:12 → 176

M_R_Ras_like; R-Ras2/TC21, M-Ras/R-Ras3

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

99267473..99319505 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006510825.4 → XP_006510888.1 ras-related protein M-Ras isoform X1

See identical proteins and their annotated locations for XP_006510888.1

UniProtKB/Swiss-Prot

O08989

UniProtKB/TrEMBL

Q3TPX5

Related

ENSMUSP00000113275.2, ENSMUST00000122384.9

Conserved Domains (1) summary

cd04145
Location:12 → 176

M_R_Ras_like; R-Ras2/TC21, M-Ras/R-Ras3
XM_036154642.1 → XP_036010535.1 ras-related protein M-Ras isoform X1

UniProtKB/Swiss-Prot

O08989

UniProtKB/TrEMBL

Q3TPX5

Related

ENSMUSP00000112407.2, ENSMUST00000119472.8

Conserved Domains (1) summary

cd04145
Location:12 → 176

M_R_Ras_like; R-Ras2/TC21, M-Ras/R-Ras3
XM_030244080.1 → XP_030099940.1 ras-related protein M-Ras isoform X1

UniProtKB/Swiss-Prot

O08989

UniProtKB/TrEMBL

Q3TPX5

Conserved Domains (1) summary

cd04145
Location:12 → 176

M_R_Ras_like; R-Ras2/TC21, M-Ras/R-Ras3