Mfng MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mfngprovided by MGI
Official Full Name: MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferaseprovided by MGI
Primary source: MGI:MGI:1095404
See related: Ensembl:ENSMUSG00000018169 AllianceGenome:MGI:1095404
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Enables O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity. Involved in marginal zone B cell differentiation and regulation of Notch signaling pathway. Acts upstream of or within blastocyst formation and positive regulation of Notch signaling pathway. Predicted to be located in Golgi membrane. Is expressed in several structures, including alimentary system; genitourinary system; limb; nervous system; and sensory organ. Orthologous to human MFNG (MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in subcutaneous fat pad adult (RPKM 50.0), mammary gland adult (RPKM 49.8) and 17 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 E1; 15 37.69 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (78640082..78657678, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (78755882..78773516, complement)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MFNG imposes a negative correlation between Jag1 and Notch, being high Jag1 in the absence of MFNG predictive of poor prognosis.
Title: Manic Fringe deficiency imposes Jagged1 addiction to intestinal tumor cells.
These results show that Notch signaling is finely calibrated in the cochlea via Lfng and Mfng to produce precisely tuned levels of signaling that first set the boundary of the organ of Corti and later regulate hair cell development.
Title: Fine-tuning of Notch signaling sets the boundary of the organ of Corti and establishes sensory cell fates.
Fringe modifications at EGF8 and EGF12 enhanced Notch1 binding to and activation from Delta-like 1, while modifications at EGF6 and EGF36 (added by Manic and Lunatic but not Radical) inhibited Notch1 activation from Jagged1.
Title: Deciphering the Fringe-Mediated Notch Code: Identification of Activating and Inhibiting Sites Allowing Discrimination between Ligands.
each Fringe contributes to T and B cell development, and Fringe is required for optimal in vitro stimulation of T and B cells.
Title: Lunatic, Manic, and Radical Fringe Each Promote T and B Cell Development.
the presence of Gal on O-fucose glycans differentially affects DLL1-induced NOTCH signaling modulated by LFNG versus MFNG
Title: Galactose differentially modulates lunatic and manic fringe effects on Delta1-induced NOTCH signaling.
Manic fringe is not required for embryonic development.
Title: Manic fringe is not required for embryonic development, and fringe family members do not exhibit redundant functions in the axial skeleton, limb, or hindbrain.
MFng colocalized with the proendocrine transcription factor Ngn3 in the developing mouse pancreas between embryonic days 9 and 14
Title: MFng is dispensable for mouse pancreas development and function.
Lunatic Fringe (Lfng) and Manic Fringe (Mfng) cooperatively enhanced the DL1-Notch2 interaction to promote marginal zone B cell development
Title: Lunatic and manic fringe cooperatively enhance marginal zone B cell precursor competition for delta-like 1 in splenic endothelial niches.
Jag1/fringe genes (Lfng, Rfng, and Mfng) may regulate postnatal bile duct growth and remodeling, and serve as candidate modifiers of the hepatic phenotype in Alagille syndrome.
Title: Bile duct proliferation in Jag1/fringe heterozygous mice identifies candidate modifiers of the Alagille syndrome hepatic phenotype.
The structure of Manic Fringe reveals amino acid residues involved in recognition of donor substrates and catalysis, and a putative binding pocket for acceptor substrates. Mutations of several residues in this pocket impair Fringe activity in vivo.
Title: Structural insights into the Notch-modifying glycosyltransferase Fringe.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (1)
Targeted (6) 1 citation

General gene information

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Markers

NoName (e-PCR)
- UniSTS:237342

Mfng (e-PCR), detects polymorphism
- UniSTS:525613

RH129110 (e-PCR)
- UniSTS:212418

AW546563 (e-PCR)
- UniSTS:160476

AI449026 (e-PCR)
- UniSTS:160477

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within blastocyst formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in marginal zone B cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pattern specification process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of Notch signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of protein binding	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of Notch signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of Notch signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: beta-1,3-N-acetylglucosaminyltransferase manic fringe

Names: O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; manic fringe homolog

NP_001398307.1

EC 2.4.1.222

NP_032621.1

EC 2.4.1.222

XP_006520614.1

EC 2.4.1.222

XP_006520615.1

EC 2.4.1.222

XP_036015108.1

EC 2.4.1.222

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001411378.1 → NP_001398307.1 beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform 2

Status: VALIDATED

Source sequence(s)

AL591946, AL592169
NM_008595.3 → NP_032621.1 beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform 1

See identical proteins and their annotated locations for NP_032621.1

Status: VALIDATED

Source sequence(s)

AL591946, AL592169

Consensus CDS

CCDS27622.1

UniProtKB/Swiss-Prot

O09008

UniProtKB/TrEMBL

Q3UPI0

Related

ENSMUSP00000018313.5, ENSMUST00000018313.6

Conserved Domains (1) summary

pfam02434
Location:51 → 300

Fringe; Fringe-like

RNA

NR_177237.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AL591946, AL592169
NR_177238.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AL591946, AL592169
NR_177239.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AL591946, AL592169
NR_177240.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AL591946, AL592169
NR_177241.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AL591946, AL592169
NR_177242.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AL591946, AL592169

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

78640082..78657678 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006520551.3 → XP_006520614.1 beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X2

See identical proteins and their annotated locations for XP_006520614.1

Conserved Domains (1) summary

pfam02434
Location:1 → 178

Fringe; Fringe-like
XM_036159215.1 → XP_036015108.1 beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X2

Conserved Domains (1) summary

pfam02434
Location:1 → 178

Fringe; Fringe-like
XM_006520552.4 → XP_006520615.1 beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X2

See identical proteins and their annotated locations for XP_006520615.1

Conserved Domains (1) summary

pfam02434
Location:1 → 178

Fringe; Fringe-like