Ubr2 ubiquitin protein ligase E3 component n-recognin 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ubr2provided by MGI
Official Full Name: ubiquitin protein ligase E3 component n-recognin 2provided by MGI
Primary source: MGI:MGI:1861099
See related: Ensembl:ENSMUSG00000023977 AllianceGenome:MGI:1861099
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: mKIAA0349; 9930021A08Rik; E130209G04Rik
Summary: Enables histone H2A ubiquitin ligase activity. Involved in several processes, including meiotic nuclear division; negative regulation of gene expression, epigenetic; and ubiquitin-dependent protein catabolic process via the N-end rule pathway. Acts upstream of or within male meiosis I and ubiquitin-dependent protein catabolic process. Located in chromatin. Part of ubiquitin ligase complex. Is expressed in several structures, including alimentary system; endocrine gland; eye; genitourinary system; and trachea. Orthologous to human UBR2 (ubiquitin protein ligase E3 component n-recognin 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in bladder adult (RPKM 12.1), thymus adult (RPKM 12.0) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 C; 17 22.91 cM

Exon count:: 48

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (47239217..47321812, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (46928291..47010886, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

UBR2 targets myosin heavy chain IIb and IIx for degradation: Molecular mechanism essential for cancer-induced muscle wasting.
Title: UBR2 targets myosin heavy chain IIb and IIx for degradation: Molecular mechanism essential for cancer-induced muscle wasting.
Taken together, our findings indicated that p53(-/-) mBMMSC exosomes could deliver UBR2 to target cells and promote gastric cancer growth and metastasis by regulating Wnt/beta-catenin pathway.
Title: UBR2 Enriched in p53 Deficient Mouse Bone Marrow Mesenchymal Stem Cell-Exosome Promoted Gastric Cancer Progression via Wnt/β-Catenin Pathway.
These data suggest that Tex19.1 and Ubr2 are required for mouse spermatocytes to accumulate sufficient Spo11-dependent recombination to ensure that the homology search is consistently successful, and reveal a hitherto unknown genetic pathway promoting meiotic recombination in mammals.
Title: Tex19.1 promotes Spo11-dependent meiotic recombination in mouse spermatocytes.
Results suggest that UBR2 up-regulation in cachectic muscle is mediated by the p38beta-C/EBPbeta signaling pathway responsible for the bulk of tumor-induced muscle proteolysis.
Title: Signaling mechanism of tumor cell-induced up-regulation of E3 ubiquitin ligase UBR2.
Results suggest that the binding of Ubr2 to Tex19.1 metabolically stabilizes Tex19.1 during spermatogenesis, revealing a new function for Ubr2 outside the conventional N-end rule pathway.
Title: The ubiquitin ligase Ubr2, a recognition E3 component of the N-end rule pathway, stabilizes Tex19.1 during spermatogenesis.
insufficiency in UBR2-dependent histone ubiquitination triggers a pachytene checkpoint system, providing a new insight into chromatin remodeling and gene expression regulation
Title: UBR2 mediates transcriptional silencing during spermatogenesis via histone ubiquitination.
UBR2 ubiquitin ligase and the N-end rule pathway are required for male meiosis and spermatogenesis and for an essential aspect of female embryonic development.
Title: Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway.
mouse fibroblast cells derived from Ubr2 deficient embryos display genome instability.
Title: Loss of Ubr2, an E3 ubiquitin ligase, leads to chromosome fragility and impaired homologous recombinational repair.
UBR1-/-UBR2-/- embryos die at midgestation, with defects in neurogenesis and cardiovascular development.
Title: Impaired neurogenesis and cardiovascular development in mice lacking the E3 ubiquitin ligases UBR1 and UBR2 of the N-end rule pathway.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1) 1 citation
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI462103 (e-PCR)
- UniSTS:181025

RH94676 (e-PCR)
- UniSTS:119923

AW540746 (e-PCR)
- UniSTS:178566

RH126638 (e-PCR)
- UniSTS:209317

Ubr2 (e-PCR), detects polymorphism
- UniSTS:525283

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables L-leucine binding	IEA Inferred from Electronic Annotation more info
enables L-leucine binding	ISO Inferred from Sequence Orthology more info
enables histone H2A ubiquitin ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular response to L-leucine	ISO Inferred from Sequence Orthology more info
involved_in cellular response to L-leucine	ISS Inferred from Sequence or Structural Similarity more info
involved_in heterochromatin formation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within male meiosis I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in male meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of TOR signaling	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of TOR signaling	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of T cell receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of T cell receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein K63-linked ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein K63-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transposable element silencing	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in ubiquitin-dependent protein catabolic process via the N-end rule pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in ubiquitin-dependent protein catabolic process via the N-end rule pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in ubiquitin-dependent protein catabolic process via the N-end rule pathway	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of ubiquitin ligase complex	IGI Inferred from Genetic Interaction more info	PubMed

General protein information

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Preferred Names: E3 ubiquitin-protein ligase UBR2

Names: N-recognin-2; RING-type E3 ubiquitin transferase UBR2; ubiquitin ligase E3 alpha-II; ubiquitin-protein ligase E3-alpha-2; ubiquitin-protein ligase E3-alpha-II

NP_001170845.1

EC 2.3.2.27

NP_666190.2

EC 2.3.2.27

XP_006524208.1

EC 2.3.2.27

XP_030105595.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001177374.2 → NP_001170845.1 E3 ubiquitin-protein ligase UBR2 isoform 2

See identical proteins and their annotated locations for NP_001170845.1

Status: VALIDATED

Source sequence(s)

AC165289, AC170807

Consensus CDS

CCDS50127.1

UniProtKB/TrEMBL

Q3UHE0, Q3UPU3

Related

ENSMUSP00000108961.3, ENSMUST00000113335.4

Conserved Domains (2) summary

pfam02207
Location:99 → 166

zf-UBR; Putative zinc finger in N-recognin (UBR box)

pfam02617
Location:222 → 300

ClpS; ATP-dependent Clp protease adaptor protein ClpS
NM_146078.4 → NP_666190.2 E3 ubiquitin-protein ligase UBR2 isoform 1

See identical proteins and their annotated locations for NP_666190.2

Status: VALIDATED

Source sequence(s)

AC165289, AC170807

Consensus CDS

CCDS28845.1

UniProtKB/Swiss-Prot

Q6DIB9, Q6WKZ8, Q80U31, Q8BUL9, Q8CGW0, Q8K2I6, Q8R0V7, Q8R130

UniProtKB/TrEMBL

Q3UHE0

Related

ENSMUSP00000108963.3, ENSMUST00000113337.10

Conserved Domains (2) summary

pfam02207
Location:99 → 166

zf-UBR; Putative zinc finger in N-recognin (UBR box)

pfam02617
Location:222 → 300

ClpS; ATP-dependent Clp protease adaptor protein ClpS

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000083.7 Reference GRCm39 C57BL/6J

Range

47239217..47321812 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006524145.3 → XP_006524208.1 E3 ubiquitin-protein ligase UBR2 isoform X2

Conserved Domains (2) summary

cd00162
Location:422 → 527

RING_Ubox; RING-H2 finger (C3H2C3-type) [structural motif]

cl17238
Location:421 → 536

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain
XM_030249735.2 → XP_030105595.1 E3 ubiquitin-protein ligase UBR2 isoform X1

Conserved Domains (2) summary

cd00162
Location:679 → 784

RING_Ubox; RING-H2 finger (C3H2C3-type) [structural motif]

cl17238
Location:678 → 793

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain