Nme8 NME/NM23 family member 8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nme8provided by MGI
Official Full Name: NME/NM23 family member 8provided by MGI
Primary source: MGI:MGI:1920662
See related: Ensembl:ENSMUSG00000041138 AllianceGenome:MGI:1920662
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Txndc3; Sptrx-2; 1700056P15Rik
Summary: Predicted to enable 3'-5' exonuclease activity and microtubule binding activity. Acts upstream of or within cellular response to reactive oxygen species and flagellated sperm motility. Located in axoneme; sperm cytoplasmic droplet; and sperm principal piece. Human ortholog(s) of this gene implicated in primary ciliary dyskinesia 6. Orthologous to human NME8 (NME/NM23 family member 8). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Restricted expression toward testis adult (RPKM 12.6) See more
Orthologs: human all
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Genomic context

Location:: 13 A2; 13 7.0 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (19829248..19881996, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (19645078..19697826, complement)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Txndc2 and Txndc3 are critically important in protecting spermatozoa against the increases in oxidative stress associated with paternal age.
Title: Functional deletion of Txndc2 and Txndc3 increases the susceptibility of spermatozoa to age-related oxidative stress.
data indicate that spermatid-specific thioredoxin-2 incorporation into the sperm fibrous sheath(FS) lags well behind FS assembly, suggesting it is required during the final stages of sperm tail maturation
Title: Cloning and developmental analysis of murid spermatid-specific thioredoxin-2 (SPTRX-2), a novel sperm fibrous sheath protein and autoantigen.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (2) 1 citation

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 3'-5' exonuclease activity	ISO Inferred from Sequence Orthology more info
enables 3'-5' exonuclease activity	ISS Inferred from Sequence or Structural Similarity more info
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to reactive oxygen species	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cilium assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in cilium assembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cilium assembly	ISO Inferred from Sequence Orthology more info
involved_in flagellated sperm motility	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within flagellated sperm motility	IGI Inferred from Genetic Interaction more info	PubMed
involved_in spermatogenesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in axoneme	IBA Inferred from Biological aspect of Ancestor more info
located_in axoneme	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of outer dynein arm	IEA Inferred from Electronic Annotation more info
part_of outer dynein arm	ISO Inferred from Sequence Orthology more info
located_in sperm cytoplasmic droplet	IDA Inferred from Direct Assay more info	PubMed
located_in sperm fibrous sheath	ISO Inferred from Sequence Orthology more info
located_in sperm midpiece	IEA Inferred from Electronic Annotation more info
located_in sperm midpiece	ISO Inferred from Sequence Orthology more info
is_active_in sperm principal piece	IBA Inferred from Biological aspect of Ancestor more info
located_in sperm principal piece	IDA Inferred from Direct Assay more info	PubMed
located_in sperm principal piece	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: thioredoxin domain-containing protein 3

Names: 3'-5' exonuclease NME8; spermatid-specific thioredoxin-2; thioredoxin domain containing 3 (spermatozoa)

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001167909.1 → NP_001161381.1 thioredoxin domain-containing protein 3 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment near the C-terminus, compared to isoform 1.

Source sequence(s)

AC153016, AC164102

Consensus CDS

CCDS49208.1

UniProtKB/Swiss-Prot

Q715T0

Related

ENSMUSP00000047052.9, ENSMUST00000039340.15

Conserved Domains (4) summary

smart00562
Location:313 → 450

NDK; Enzymes that catalyze nonsubstrate specific conversions of nucleoside diphosphates to nucleoside triphosphates

cd02948
Location:11 → 113

TRX_NDPK; TRX domain, TRX and NDP-kinase (NDPK) fusion protein family; most members of this group are fusion proteins which contain one redox active TRX domain containing a CXXC motif and three NDPK domains, and are characterized as intermediate chains (ICs) of ...

cd04416
Location:313 → 445

NDPk_TX; NDP kinase domain of thioredoxin domain-containing proteins (TXNDC3 and TXNDC6): Txl-2 (TXNDC6) and Sptrx-2 (TXNDC3) are fusion proteins of Group II N-terminal thioredoxin domains followed by one or three NDP kinase domains, respectively. Sptrx-2, which ...

cl00335
Location:155 → 301

NDPk; Nucleoside diphosphate kinases (NDP kinases, NDPks): NDP kinases, responsible for the synthesis of nucleoside triphosphates (NTPs), are involved in numerous regulatory processes associated with proliferation, development, and differentiation. They are ...
NM_181591.3 → NP_853622.2 thioredoxin domain-containing protein 3 isoform 1

See identical proteins and their annotated locations for NP_853622.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC153016, AC164102

Consensus CDS

CCDS26262.2

UniProtKB/Swiss-Prot

Q715T0, Q80W74

Related

ENSMUSP00000089358.3, ENSMUST00000091763.3

Conserved Domains (4) summary

smart00562
Location:448 → 581

NDK; Enzymes that catalyze nonsubstrate specific conversions of nucleoside diphosphates to nucleoside triphosphates

cd02948
Location:11 → 113

TRX_NDPK; TRX domain, TRX and NDP-kinase (NDPK) fusion protein family; most members of this group are fusion proteins which contain one redox active TRX domain containing a CXXC motif and three NDPK domains, and are characterized as intermediate chains (ICs) of ...

cd04416
Location:313 → 445

NDPk_TX; NDP kinase domain of thioredoxin domain-containing proteins (TXNDC3 and TXNDC6): Txl-2 (TXNDC6) and Sptrx-2 (TXNDC3) are fusion proteins of Group II N-terminal thioredoxin domains followed by one or three NDP kinase domains, respectively. Sptrx-2, which ...

cl00335
Location:448 → 579

NDPk; Nucleoside diphosphate kinases (NDP kinases, NDPks): NDP kinases, responsible for the synthesis of nucleoside triphosphates (NTPs), are involved in numerous regulatory processes associated with proliferation, development, and differentiation. They are ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000079.7 Reference GRCm39 C57BL/6J

Range

19829248..19881996 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011244353.3 → XP_011242655.1 thioredoxin domain-containing protein 3 isoform X2

Related

ENSMUSP00000152780.2, ENSMUST00000223466.2

Conserved Domains (2) summary

cd04416
Location:97 → 229

NDPk_TX; NDP kinase domain of thioredoxin domain-containing proteins (TXNDC3 and TXNDC6): Txl-2 (TXNDC6) and Sptrx-2 (TXNDC3) are fusion proteins of Group II N-terminal thioredoxin domains followed by one or three NDP kinase domains, respectively. Sptrx-2, which ...

cl00335
Location:232 → 363

NDPk; Nucleoside diphosphate kinases (NDP kinases, NDPks): NDP kinases, responsible for the synthesis of nucleoside triphosphates (NTPs), are involved in numerous regulatory processes associated with proliferation, development, and differentiation. They are ...
XM_017315615.2 → XP_017171104.1 thioredoxin domain-containing protein 3 isoform X1

Related

ENSMUST00000223286.2

Conserved Domains (3) summary

cd02948
Location:11 → 113

TRX_NDPK; TRX domain, TRX and NDP-kinase (NDPK) fusion protein family; most members of this group are fusion proteins which contain one redox active TRX domain containing a CXXC motif and three NDPK domains, and are characterized as intermediate chains (ICs) of ...

cd04416
Location:313 → 445

NDPk_TX; NDP kinase domain of thioredoxin domain-containing proteins (TXNDC3 and TXNDC6): Txl-2 (TXNDC6) and Sptrx-2 (TXNDC3) are fusion proteins of Group II N-terminal thioredoxin domains followed by one or three NDP kinase domains, respectively. Sptrx-2, which ...

cl00335
Location:155 → 301

NDPk; Nucleoside diphosphate kinases (NDP kinases, NDPks): NDP kinases, responsible for the synthesis of nucleoside triphosphates (NTPs), are involved in numerous regulatory processes associated with proliferation, development, and differentiation. They are ...
XM_017315616.2 → XP_017171105.1 thioredoxin domain-containing protein 3 isoform X3

Conserved Domains (2) summary

cd02948
Location:11 → 113

TRX_NDPK; TRX domain, TRX and NDP-kinase (NDPK) fusion protein family; most members of this group are fusion proteins which contain one redox active TRX domain containing a CXXC motif and three NDPK domains, and are characterized as intermediate chains (ICs) of ...

cl00335
Location:155 → 255

NDPk; Nucleoside diphosphate kinases (NDP kinases, NDPks): NDP kinases, responsible for the synthesis of nucleoside triphosphates (NTPs), are involved in numerous regulatory processes associated with proliferation, development, and differentiation. They are ...