asf1 anti-silencing factor 1 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: asf1provided by FlyBase
Official Full Name: anti-silencing factor 1provided by FlyBase
Primary source: FLYBASE:FBgn0029094
Locus tag: Dmel_CG9383
See related: AllianceGenome:FB:FBgn0029094
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: ASF-1; Asf1; ASF1; CG9383; dASF/SF2; dAsf1; dASF1; Dmel\CG9383; l(3)76BDq
Summary: Enables RNA polymerase II-specific DNA-binding transcription factor binding activity. Involved in DNA replication-dependent chromatin assembly; negative regulation of transcription by RNA polymerase II; and regulation of mRNA splicing, via spliceosome. Located in polytene chromosome. Part of RCAF complex and transcription repressor complex. Is expressed in several structures, including antennal primordium; embryonic/larval midgut primordium; ganglia; head epidermis primordium; and mesoderm. Orthologous to several human genes including ASF1A (anti-silencing function 1A histone chaperone). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 76B9-76B9; 3-45 cM

Exon count:: 1

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (19618894..19620398)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (19611994..19613498)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Genetic and Molecular Interactions between H[DeltaCT], a Novel Allele of the Notch Antagonist Hairless, and the Histone Chaperone Asf1 in Drosophila melanogaster.
Title: Genetic and Molecular Interactions between H(ΔCT), a Novel Allele of the Notch Antagonist Hairless, and the Histone Chaperone Asf1 in Drosophila melanogaster.
ASF1 is critically required to load H3.3-H4 dimers on the HIRA complex prior to histone deposition on paternal DNA. This separation of tasks could optimize the rapid assembly of paternal chromatin within the gigantic volume of the egg cell. In contrast, ASF1 is surprisingly dispensable for the amplification of cleavage nuclei, although chromatin integrity is likely compromised in asf1 knocked-down embryos.
Title: ASF1 is required to load histones on the HIRA complex in preparation of paternal chromatin assembly at fertilization.
DLP and ASF1 are part of a predeposition complex, which is recruited by XNP and is necessary to prevent DNA exposure in the nucleus.
Title: The Drosophila DAXX-Like Protein (DLP) Cooperates with ASF1 for H3.3 Deposition and Heterochromatin Formation.
ASF1 cellular levels are tightly controlled by distinct pathways and provide a molecular mechanism for post-translational regulation of dASF1 and hASF1a by TLK kinases.
Title: Phosphorylation-mediated control of histone chaperone ASF1 levels by Tousled-like kinases.
Histone chaperones ASF1 and NAP1 differentially modulate removal of active histone marks by LID-RPD3 complexes during NOTCH silencing.
Title: Histone chaperones ASF1 and NAP1 differentially modulate removal of active histone marks by LID-RPD3 complexes during NOTCH silencing.
Asf1 is directed via interactions with sequence-specific complexes to mediate silencing of specific target genes.
Title: Gene-specific targeting of the histone chaperone asf1 to mediate silencing.
dASF1 has a direct role in modifying chromatin structure during DNA replication and this function of dASF1 is important for the processivity of the replication machinery.
Title: The histone chaperone ASF1 localizes to active DNA replication forks to mediate efficient DNA replication.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables histone binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA replication-dependent chromatin assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication-dependent chromatin assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin organization	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mRNA splicing, via spliceosome	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of RCAF complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromatin	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in polytene chromosome	IDA Inferred from Direct Assay more info	PubMed
part_of transcription repressor complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: anti-silencing factor 1

Names: CG9383-PA; CG9383-PB; anti- silencing function 1; anti-silencing factor; asf1-PA; asf1-PB

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

19618894..19620398

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079439.4 → NP_524163.1 anti-silencing factor 1, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524163.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9V464

UniProtKB/TrEMBL

C0MIY4

Related

FBpp0074715

Conserved Domains (1) summary

pfam04729
Location:1 → 154

ASF1_hist_chap; ASF1 like histone chaperone
NM_001202202.2 → NP_001189131.1 anti-silencing factor 1, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001189131.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9V464

UniProtKB/TrEMBL

C0MIY4

Conserved Domains (1) summary

pfam04729
Location:1 → 154

ASF1_hist_chap; ASF1 like histone chaperone