DSK2 ubiquitin domain-containing protein DSK2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DSK2
Official Full Name: ubiquitin domain-containing protein DSK2
Primary source: SGD:S000004889
Locus tag: YMR276W
See related: AllianceGenome:SGD:S000004889; FungiDB:YMR276W; VEuPathDB:YMR276W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables K48-linked polyubiquitin modification-dependent protein binding activity and protein-macromolecule adaptor activity. Involved in ERAD pathway; protein localization to vacuole; and spindle pole body duplication. Predicted to be located in nucleus. Predicted to be active in cytosol. Used to study amyotrophic lateral sclerosis type 15. Human ortholog(s) of this gene implicated in amyotrophic lateral sclerosis and amyotrophic lateral sclerosis type 15. Orthologous to several human genes including UBQLN2 (ubiquilin 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See DSK2 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (818827..819948)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Identify the Cdc48-Rad23/Dsk2 axis as a major route to the proteasome.
Title: In Vivo Ubiquitin Linkage-type Analysis Reveals that the Cdc48-Rad23/Dsk2 Axis Contributes to K48-Linked Chain Specificity of the Proteasome.
Proteasome/autophagy pathway choice is independent of the ubiquitin-binding properties of the receptors Dsk2/Cue5 but largely determined by their oligomerization potentials.
Title: Receptor oligomerization guides pathway choice between proteasomal and autophagic degradation.
functional yeast proteasomes have the capacity to associate and dissociate with Rpn10 and that Rpn10 monoubiquitination decreases the Rpn10-proteasome and Rpn10-Dsk2 associations.
Title: Rpn10 monoubiquitination orchestrates the association of the ubiquilin-type DSK2 receptor with the proteasome.
C-terminal UBA domain of Dsk2 ubiquitin receptor protects it by preventing initiation of protein degradation at the proteasome.
Title: C-terminal UBA domains protect ubiquitin receptors by preventing initiation of protein degradation.
These results indicate that ubiquitination in the UBL domain of Dsk2 has in vivo functions in the ubiquitin-proteasome pathway in yeast.
Title: Ubiquitin chains in the Dsk2 UBL domain mediate Dsk2 stability and protein degradation in yeast.
Data present the crystal structures of Ufd2 in complex with the UBL domains of Rad23 and Dsk2.
Title: The yeast E4 ubiquitin ligase Ufd2 interacts with the ubiquitin-like domains of Rad23 and Dsk2 via a novel and distinct ubiquitin-like binding domain.
Data describe formation of ternary complexes of Dsk2 and Rpn10 with (poly)ubiquitin and found that, depending on the ubiquitin chain length, the orientation of the resulting complex is entirely different, providing for alternate signals.
Title: Together, Rpn10 and Dsk2 can serve as a polyubiquitin chain-length sensor.
Using a genome-wide functional screen, we have identified 11 yeast genes that cause slower growth upon their overexpression in cells lacking two ubiquitin-binding proteins Rad23 and Dsk2.
Title: A genome-wide synthetic dosage lethality screen reveals multiple pathways that require the functioning of ubiquitin-binding proteins Rad23 and Dsk2.
Dsk2p, Rad23p and the trimeric Cdc48 complex function together in the delivery of ubiquitinated proteins to the proteasome
Title: A genomic screen identifies Dsk2p and Rad23p as essential components of ER-associated degradation.
structure reveals that the UBA domain uses a mode of ubiquitin recognition that is similar to that of the CUE domain, another ubiquitin binding motif that shares low sequence homology but high structural similarity with UBA domains
Title: Structure of the UBA domain of Dsk2p in complex with ubiquitin molecular determinants for ubiquitin recognition.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables K48-linked polyubiquitin modification-dependent protein binding	IDA Inferred from Direct Assay more info	PubMed
enables polyubiquitin modification-dependent protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein-macromolecule adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in ERAD pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to vacuole	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spindle pole body duplication	IGI Inferred from Genetic Interaction more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IC Inferred by Curator more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ubiquitin domain-containing protein DSK2

NP_014003.1

Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; K48-Ub chain binding protein that also binds K63-linked chains in vitro; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; contains an N-terminal Ub-like (UBL) and a C-terminal Ub-associated (UBA) domain; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

818827..819948

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182783.1 → NP_014003.1 TPA: ubiquitin domain-containing protein DSK2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014003.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0A3, P48510

UniProtKB/TrEMBL

B3LMG4, B5VQ43, C7GTM3, C8ZFE3

Conserved Domains (3) summary

smart00727
Location:149 → 185

STI1; Heat shock chaperonin-binding motif

cd14324
Location:328 → 369

UBA_Dsk2p_like; UBA domain of Saccharomyces cerevisiae proteasome interacting protein Dsk2p and its homologs found in fungi

cd16106
Location:3 → 74

Ubl_Dsk2p_like; ubiquitin-like (Ubl) domain found in Saccharomyces cerevisiae proteasome interacting protein Dsk2p and similar proteins