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    ase1 antiparallel microtubule cross-linking factor Ase1 [ Schizosaccharomyces pombe (fission yeast) ]

    Gene ID: 2543372, updated on 18-Sep-2024

    Summary

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    Official Symbol
    ase1
    Official Full Name
    antiparallel microtubule cross-linking factor Ase1
    Primary source
    PomBase:SPAPB1A10.09
    Locus tag
    SPOM_SPAPB1A10.09
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Schizosaccharomyces pombe (strain: 972h-)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
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    Genomic context

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    See ase1 in Genome Data Viewer
    Location:
    chromosome: I
    Exon count:
    2
    Sequence:
    Chromosome: I; NC_003424.3 (1878189..1880726)

    Chromosome I - NC_003424.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene uncharacterized protein Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene GTPase Ypt71 Neighboring gene glutamyl--tRNA (Glu) ligase Mse1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003424.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Diffusible crosslinkers generate directed forces in microtubule networks. Lansky Z, et al. Cell, 2015 Mar 12. PMID 25748652
    2. Microtubule-driven multimerization recruits ase1p onto overlapping microtubules. Kapitein LC, et al. Curr Biol, 2008 Nov 11. PMID 18976915
    3. Latrunculin A delays anaphase onset in fission yeast by disrupting an Ase1-independent pathway controlling mitotic spindle stability. Meadows JC, et al. Mol Biol Cell, 2008 Sep. PMID 18562692, Free PMC Article
    4. Ase1p organizes antiparallel microtubule arrays during interphase and mitosis in fission yeast. Loïodice I, et al. Mol Biol Cell, 2005 Apr. PMID 15689489, Free PMC Article
    5. Adaptive braking by Ase1 prevents overlapping microtubules from sliding completely apart. Braun M, et al. Nat Cell Biol, 2011 Sep 4. PMID 21892183

    See all (41) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Klp2 and Ase1 synergize to maintain meiotic spindle stability during metaphase I.
      Title: Klp2 and Ase1 synergize to maintain meiotic spindle stability during metaphase I.
    2. Kinesin-6 Klp9 plays motor-dependent and -independent roles in collaboration with Kinesin-5 Cut7 and the microtubule crosslinker Ase1 in fission yeast.
      Title: Kinesin-6 Klp9 plays motor-dependent and -independent roles in collaboration with Kinesin-5 Cut7 and the microtubule crosslinker Ase1 in fission yeast.
    3. Study shows that fission yeast bipolar spindle assembly and chromosome segregation can take place in the absence of both kinesin-5 Cut7 and kinesin-14 Pkl1. In cut7Deltapkl1Delta cells, spindle formation and bipolarity requires the microtubule (MT) antiparallel bundler Ase1 to recruit Cls1 to stabilize MTs.
      Title: Kinesin-5-independent mitotic spindle assembly requires the antiparallel microtubule crosslinker Ase1 in fission yeast.
    4. Study demonstrated that diffusible microtubule crosslinker Ase1 generated directed microtubule sliding when confined between partially overlapping microtubules. The Ase1-generated forces, directly measured by optical tweezers to be in the piconewton-range, were sufficient to antagonize motor-protein driven microtubule sliding.
      Title: Diffusible crosslinkers generate directed forces in microtubule networks.
    5. Show that Ase1, a member of the conserved MAP65/PRC1 family of microtubule-bundling proteins, enables the formation of stable antiparallel overlaps through adaptive braking of Kinesin-14-driven microtubule-microtubule sliding.
      Title: Adaptive braking by Ase1 prevents overlapping microtubules from sliding completely apart.
    6. An Ase1/Prc1-dependent mechanism that prevents spindle collapse and thus asymmetric division is required for correction of merotelic attachment in anaphase.
      Title: Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast.
    7. Data show that fission yeast kinesin-6 motor klp9p binds to the microtubule antiparallel bundler ase1p at the midzone at anaphase B onset.
      Title: Phospho-regulated interaction between kinesin-6 Klp9p and microtubule bundler Ase1p promotes spindle elongation.
    8. loss of Ase1 activates a checkpoint that requires only the Mad3, Bub1, and Mph1, but not Mad1, Mad2, or Bub3 checkpoint proteins
      Title: Latrunculin A delays anaphase onset in fission yeast by disrupting an Ase1-independent pathway controlling mitotic spindle stability.
    9. Cells use a finely tuned cooperative localization mechanism that exploits differences in the geometry and concentration of ase1p binding sites along single and overlapping microtubules.
      Title: Microtubule-driven multimerization recruits ase1p onto overlapping microtubules.
    10. ase1 has a role in mitotic cell division, meiotic nuclear oscillation, and cytokinesis checkpoint signaling
      Title: The roles of fission yeast ase1 in mitotic cell division, meiotic nuclear oscillation, and cytokinesis checkpoint signaling.
    Submit: New GeneRIF Correction See all GeneRIFs (11)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by PomBase

    Function Evidence Code Pubs
    enables microtubule binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables microtubule binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables microtubule lateral binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in dynein-driven meiotic oscillatory nuclear movement IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in horsetail-astral microtubule organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in meiotic spindle elongation EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in meiotic spindle formation (spindle phase two) IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in microtubule bundle formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in microtubule cytoskeleton organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mitotic cytokinesis checkpoint signaling IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in mitotic nuclear bridge organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic spindle elongation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic spindle formation (spindle phase one) IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic spindle formation (spindle phase two) IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic spindle midzone assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mitotic spindle midzone assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in mitotic spindle midzone assembly IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in mitotic spindle midzone assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of plus-end directed microtubule sliding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in nuclear migration by microtubule mediated pushing forces IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cortical microtubule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in cytoplasmic microtubule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in equatorial microtubule organizing center IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in meiotic spindle midzone IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    is_active_in microtubule cytoskeleton HDA PubMed 
    is_active_in mitotic spindle microtubule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in mitotic spindle midzone IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in mitotic spindle midzone IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus IC
    Inferred by Curator
    more info
    		 PubMed 
    is_active_in polar microtubule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in post-anaphase array microtubule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in post-anaphase microtubule array EXP
    Inferred from Experiment
    more info
    		 PubMed 
    is_active_in spindle IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in static microtubule bundle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    antiparallel microtubule cross-linking factor Ase1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003424.3 Reference assembly

      Range
      1878189..1880726
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001018957.3 → NP_593523.1  antiparallel microtubule cross-linking factor Ase1 [Schizosaccharomyces pombe]

      See identical proteins and their annotated locations for NP_593523.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      Q9HDY1
      Conserved Domains (2) summary
      pfam03999
      Location:72 → 649
      MAP65_ASE1; Microtubule associated protein (MAP65/ASE1 family)
      pfam16919
      Location:465 → 525
      PknG_rubred; Protein kinase G rubredoxin domain
    Nucleotide Protein
    Heading Accession and Version

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