Prkch protein kinase C, eta [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkchprovided by MGI
Official Full Name: protein kinase C, etaprovided by MGI
Primary source: MGI:MGI:97600
See related: Ensembl:ENSMUSG00000021108 AllianceGenome:MGI:97600
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pkch
Summary: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in the human gene are associated with susceptibility to cerebral infarction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
Expression: Broad expression in lung adult (RPKM 17.2), thymus adult (RPKM 13.2) and 21 other tissues See more
Orthologs: human all
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Genomic context

Location:: 12 C3; 12 30.81 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	12	NC_000078.7 (73631570..73824959)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	12	NC_000078.6 (73584796..73778185)

Chromosome 12 - NC_000078.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

An miRNA derived from amelogenin exon4 regulates expression of transcription factor Runx2 by directly targeting upstream activators Nfia and Prkch.
Title: An miRNA derived from amelogenin exon4 regulates expression of transcription factor Runx2 by directly targeting upstream activators Nfia and Prkch.
These findings reveal a novel Rictor-Prkch-Klf4 pathway that plays an important role in the regulation of Embryonic stem cells differentiation
Title: Superhero Rictor promotes cellular differentiation of mouse embryonic stem cells.
genetic deficiency results in defective suppressive activity of T reg cells
Title: Protein kinase C-η controls CTLA-4-mediated regulatory T cell function.
data suggest that PKCeta shares some redundant roles with PKCtheta; in T cell biology and also performs nonredundant functions that are required for T cell homeostasis and activation
Title: Protein kinase C η is required for T cell activation and homeostatic proliferation.
Protein kinase C eta promotes a proliferation to differentiation switch in keratinocytes via upregulation of p27Kip1 mRNA through suppression of JNK/c-Jun signaling under stress conditions.
Title: PKCη promotes a proliferation to differentiation switch in keratinocytes via upregulation of p27Kip1 mRNA through suppression of JNK/c-Jun signaling under stress conditions.
these results establish a previously uncharacterized role forPKC-epsilon, PKC-eta, PKC-theta in T cell polarity.
Title: A cascade of protein kinase C isozymes promotes cytoskeletal polarization in T cells.
ATP1A1 interaction with PKCeta and occludin was involved in the integrity of the blood-labyrinth-barrier
Title: Na+/K+-ATPase α1 identified as an abundant protein in the blood-labyrinth barrier that plays an essential role in the barrier integrity.
Translational regulation could provide an additional level for controlling the expression of PKC family members.
Title: Translational control of protein kinase Ceta by two upstream open reading frames.
directs the induction of IRF-4 expression downstream of BLNK in the pre-BCR signaling pathway promoting kappa gene rearrangement
Title: PKC eta directs induction of IRF-4 expression and Ig kappa gene rearrangement in pre-BCR signaling pathway.
nPKCeta positively regulates agonist-induced thromboxane generation with no effects on platelet aggregation.
Title: Mechanism of activation and functional role of protein kinase Ceta in human platelets.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Prkch (e-PCR), detects polymorphism
- UniSTS:144197

PMC109105P1 (e-PCR)
- UniSTS:270153

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables calcium,diacylglycerol-dependent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase binding	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed
enables translation regulator activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in cellular response to amino acid starvation	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of glial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of glial cell apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of translation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of B cell receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of B cell receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of glial cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of glial cell proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of keratinocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of macrophage derived foam cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of translation in response to stress	ISO Inferred from Sequence Orthology more info
involved_in protein kinase C signaling	IEA Inferred from Electronic Annotation more info
involved_in protein kinase C signaling	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of bicellular tight junction assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of bicellular tight junction assembly	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: protein kinase C eta type

Names: PKC-L; nPKC-eta

NP_001300906.1

EC 2.7.11.13

NP_032882.2

EC 2.7.11.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001313977.1 → NP_001300906.1 protein kinase C eta type isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.

Source sequence(s)

AC124179, AK164044, BF462787

UniProtKB/TrEMBL

Q3U1I0

Conserved Domains (2) summary

cd05590
Location:246 → 568

STKc_nPKC_eta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C eta

pfam00130
Location:133 → 185

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
NM_008856.4 → NP_032882.2 protein kinase C eta type isoform 1

See identical proteins and their annotated locations for NP_032882.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AC124179, AK145817, AK164044, BF462787

Consensus CDS

CCDS25976.1

UniProtKB/Swiss-Prot

P23298, Q8K2K8

UniProtKB/TrEMBL

Q3UKY1

Related

ENSMUSP00000021527.9, ENSMUST00000021527.15

Conserved Domains (3) summary

cd04014
Location:8 → 140

C2_PKC_epsilon; C2 domain in Protein Kinase C (PKC) epsilon

cd05590
Location:359 → 681

STKc_nPKC_eta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C eta

pfam00130
Location:246 → 296

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)