Cplx2 complexin 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cplx2provided by MGI
Official Full Name: complexin 2provided by MGI
Primary source: MGI:MGI:104726
See related: Ensembl:ENSMUSG00000025867 Ensembl:ENSMUSG00000112941 AllianceGenome:MGI:104726
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 921-L; Gm34843
Summary: Predicted to enable calcium-dependent protein binding activity and syntaxin-1 binding activity. Involved in positive regulation of synaptic plasticity and regulation of synaptic vesicle fusion to presynaptic active zone membrane. Is active in calyx of Held and glutamatergic synapse. Is expressed in nervous system; retina layer; and skeletal muscle. Used to study schizophrenia. Orthologous to human CPLX2 (complexin 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in cortex adult (RPKM 102.7), frontal lobe adult (RPKM 85.8) and 7 other tissues See more
Orthologs: human all
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Genomic context

Location:: 13 B1; 13 28.59 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (54455447..54531736)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (54301964..54383923)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Long non-coding RNA LncCplx2 regulates glucose homeostasis and pancreatic beta cell function.
Title: Long non-coding RNA LncCplx2 regulates glucose homeostasis and pancreatic β cell function.
Complexin Suppresses Spontaneous Exocytosis by Capturing the Membrane-Proximal Regions of VAMP2 and SNAP25.
Title: Complexin Suppresses Spontaneous Exocytosis by Capturing the Membrane-Proximal Regions of VAMP2 and SNAP25.
data suggest that CPLX2 inhibits spontaneous secretion of IgM and IgG1 from splenic antibody-secreting cells (ASCs). This study provides new insight into the mechanism of antibody secretion of ASCs.
Title: Complexin 2 regulates secretion of immunoglobulin in antibody-secreting cells.
the C2B domain of syt1, independent of complexin, is the molecular clamp that arrests synaptic vesicles prior to Ca(2+)-triggered fusion.
Title: Synaptotagmin 1 clamps synaptic vesicle fusion in mammalian neurons independent of complexin.
We describe a high degree of structural similarity between the CpxII CTD and the SNAP25-SN1 domain (C-terminal half) and show that the CTD peptide lowers the rate of SDS-resistant SNARE complex formation in vitro. Moreover, corresponding CpxII:SNAP25 chimeras do restore complexin's function and even 'superclamp' tonic secretion.
Title: A mechanism for exocytotic arrest by the Complexin C-terminus.
Complexin II is a central target molecule that links NADPH oxidase-derived reactive oxygen species to glutamate-mediated neuronal excitotoxicity in ischemic stroke.
Title: NOX2 deficiency ameliorates cerebral injury through reduction of complexin II-mediated glutamate excitotoxicity in experimental stroke.
CpxII attenuates fluctuations of the early fusion pore and slows its expansion.
Title: Complexin synchronizes primed vesicle exocytosis and regulates fusion pore dynamics.
Cplx 1 and 2 play a role in facilitating vesicle priming, and also lead to the new hypothesis that Cplxs may synchronize vesicle release by promoting coupling between secretory vesicles and calcium channels.
Title: Complexin facilitates exocytosis and synchronizes vesicle release in two secretory model systems.
Cplx2 knockout mice exhibit hyperlocomotion following neonatal isolation stress.
Title: [Effects of neonatal isolation stress on hyperlocomotion of complexin II knockout mouse].
Several months after lesion, Cplx2 null mutants showed reduced pre-pulse inhibition, deficit of spatial learning and loss of inhibition after MK-801 challenge.
Title: Complexin2 null mutation requires a 'second hit' for induction of phenotypic changes relevant to schizophrenia.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

U35101 (e-PCR)
- UniSTS:125730

AW492120 (e-PCR)
- UniSTS:180535

CPLX2_9184 (e-PCR)
- UniSTS:468355

D38613 (e-PCR)
- UniSTS:163536

AI413745 (e-PCR)
- UniSTS:165874

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables SNARE binding	IBA Inferred from Biological aspect of Ancestor more info
enables SNARE binding	ISO Inferred from Sequence Orthology more info
enables calcium-dependent protein binding	IEA Inferred from Electronic Annotation more info
enables calcium-dependent protein binding	ISO Inferred from Sequence Orthology more info
enables syntaxin-1 binding	ISO Inferred from Sequence Orthology more info	PubMed

Process	Evidence Code	Pubs
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in mast cell degranulation	IEA Inferred from Electronic Annotation more info
involved_in modulation of chemical synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in nervous system development	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of synaptic plasticity	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of synaptic vesicle fusion to presynaptic active zone membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of synaptic vesicle fusion to presynaptic active zone membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of synaptic vesicle fusion to presynaptic active zone membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic vesicle exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic vesicle exocytosis	IEA Inferred from Electronic Annotation more info
involved_in synaptic vesicle exocytosis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of SNARE complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SNARE complex	ISO Inferred from Sequence Orthology more info
is_active_in calyx of Held	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in dendrite	ISO Inferred from Sequence Orthology more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in perikaryon	IEA Inferred from Electronic Annotation more info
located_in postsynapse	IEA Inferred from Electronic Annotation more info
is_active_in postsynapse	ISO Inferred from Sequence Orthology more info
is_active_in presynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynapse	ISO Inferred from Sequence Orthology more info
located_in synapse	ISO Inferred from Sequence Orthology more info	PubMed
part_of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex	IEA Inferred from Electronic Annotation more info
part_of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex	ISO Inferred from Sequence Orthology more info
is_active_in terminal bouton	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: complexin-2

Names: CPX II; complexin II; synaphin-1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001362218.1 → NP_001349147.1 complexin-2

Status: VALIDATED

Source sequence(s)

AC242674, AW492120, CJ108367, CX240169

Consensus CDS

CCDS36667.1

UniProtKB/Swiss-Prot

P84086, Q32KK4

Conserved Domains (1) summary

pfam05835
Location:1 → 133

Synaphin; Synaphin protein
NM_009946.3 → NP_034076.1 complexin-2

See identical proteins and their annotated locations for NP_034076.1

Status: VALIDATED

Source sequence(s)

AC242674, BN000502

Consensus CDS

CCDS36667.1

UniProtKB/Swiss-Prot

P84086, Q32KK4

Related

ENSMUSP00000026985.9, ENSMUST00000026985.9

Conserved Domains (1) summary

pfam05835
Location:1 → 133

Synaphin; Synaphin protein

RNA

NR_155492.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

BE984504, BM941693, CJ108367, CX240169

Related

ENSMUST00000240905.2

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000079.7 Reference GRCm39 C57BL/6J

Range

54455447..54531736

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017315376.3 → XP_017170865.1 complexin-2 isoform X1

UniProtKB/Swiss-Prot

P84086, Q32KK4

Conserved Domains (1) summary

pfam05835
Location:1 → 133

Synaphin; Synaphin protein