Cd8a CD8 subunit alpha [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cd8aprovided by MGI
Official Full Name: CD8 subunit alphaprovided by MGI
Primary source: MGI:MGI:88346
See related: Ensembl:ENSMUSG00000053977 AllianceGenome:MGI:88346
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ly-2; Ly-B; Ly-35; Lyt-2
Summary: Enables identical protein binding activity. Acts upstream of or within several processes, including cytotoxic T cell differentiation; defense response to virus; and positive regulation of calcium-mediated signaling. Located in external side of plasma membrane. Part of receptor complex. Is expressed in several structures, including endocrine gland; exocrine gland; genitourinary system; gut; and immune system. Human ortholog(s) of this gene implicated in primary immunodeficiency disease. Orthologous to human CD8A (CD8 subunit alpha). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Restricted expression toward thymus adult (RPKM 213.1) See more
Orthologs: human all
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Genomic context

Location:: 6 C1; 6 32.14 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (71350411..71356155)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (71373427..71379171)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A Cryptosporidium parvum vaccine candidate effect on immunohistochemical profiling of CD4[+], CD8[+], Caspase-3 and NF-kappaB in mice.
Title: A Cryptosporidium parvum vaccine candidate effect on immunohistochemical profiling of CD4(+), CD8(+), Caspase-3 and NF-κB in mice.
The CD8alpha-PILRalpha interaction maintains CD8(+) T cell quiescence.
Title: The CD8α-PILRα interaction maintains CD8(+) T cell quiescence.
Ribosylation of the CD8alphabeta heterodimer permits binding of the nonclassical major histocompatibility molecule, H2-Q10.
Title: Ribosylation of the CD8αβ heterodimer permits binding of the nonclassical major histocompatibility molecule, H2-Q10.
Positron emission tomography imaging with (89)Zr-labeled anti-CD8 cys-diabody reveals CD8(+) cell infiltration during oncolytic virus therapy in a glioma murine model.
Title: Positron emission tomography imaging with (89)Zr-labeled anti-CD8 cys-diabody reveals CD8(+) cell infiltration during oncolytic virus therapy in a glioma murine model.
IL-4Ralpha signaling by CD8alpha(+) dendritic cells contributes to cerebral malaria by enhancing inflammatory, Th1, and cytotoxic CD8(+) T cell responses.
Title: IL-4Rα signaling by CD8α(+) dendritic cells contributes to cerebral malaria by enhancing inflammatory, Th1, and cytotoxic CD8(+) T cell responses.
Constitutive CD8 expression drives innate CD8(+) T-cell differentiation via induction of iNKT2 cells.
Title: Constitutive CD8 expression drives innate CD8(+) T-cell differentiation via induction of iNKT2 cells.
Intestinal CD8alphaalpha IELs derived from two distinct thymic precursors have staggered ontogeny.
Title: Intestinal CD8αα IELs derived from two distinct thymic precursors have staggered ontogeny.
CD8alpha-deficient conventional dendritic cells control Vbeta T-cell immunity in response to Staphylococcus aureus infection in mice.
Title: CD8α(-) conventional dendritic cells control Vβ T-cell immunity in response to Staphylococcus aureus infection in mice.
results suggest that CD4 CD8 double knockout (DN)T cells can develop efficiently in vivo and chronic exposure to bacterial superantigens may precipitate a lupus-like autoimmune disease through activation of DNT cells
Title: Concomitant Disruption of CD4 and CD8 Genes Facilitates the Development of Double Negative αβ TCR(+) Peripheral T Cells That Respond Robustly to Staphylococcal Superantigen.
Depletion of Depletion of medullary thymic epithelial cells (mTECs) and CD8 antigen alpha chain (CD8alpha+) dendritic cells (DCs) leads to overt autoimmunity.
Title: Medullary thymic epithelial cells and CD8α(+) dendritic cells coordinately regulate central tolerance but CD8α(+) cells are dispensable for thymic regulatory T cell production.

Submit: New GeneRIF Correction See all GeneRIFs (93)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (2)
Endonuclease-mediated (10) 1 citation
Not Applicable (1)
Targeted (7) 1 citation

General gene information

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Markers

Y00157 (e-PCR)
- UniSTS:159518

BB154331 (e-PCR)
- UniSTS:185670

PMC122808P1 (e-PCR)
- UniSTS:270412

Y00157 (e-PCR)
- UniSTS:125906

Cd8a (e-PCR), detects polymorphism
- UniSTS:141298

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables MHC class I protein complex binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in T cell activation	ISO Inferred from Sequence Orthology more info
involved_in T cell activation	ISS Inferred from Sequence or Structural Similarity more info
involved_in T cell mediated immunity	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within T cell mediated immunity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in T cell receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in T cell receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in adaptive immune response	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of calcium-mediated signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in cell surface receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cell surface receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in cytotoxic T cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cytotoxic T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within defense response to virus	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of calcium-mediated signaling	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	ISO Inferred from Sequence Orthology more info
is_active_in external side of plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane raft	ISO Inferred from Sequence Orthology more info
part_of receptor complex	IPI Inferred from Physical Interaction more info	PubMed
part_of receptor complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: T-cell surface glycoprotein CD8 alpha chain

Names: CD8 antigen, alpha chain; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell surface glycoprotein Lyt-2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001081110.2 → NP_001074579.1 T-cell surface glycoprotein CD8 alpha chain isoform 1 precursor

See identical proteins and their annotated locations for NP_001074579.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC160090

Consensus CDS

CCDS39507.1

UniProtKB/Swiss-Prot

P01731

UniProtKB/TrEMBL

Q542K6

Related

ENSMUSP00000068123.8, ENSMUST00000066747.14

Conserved Domains (2) summary

cd05720
Location:41 → 147

Ig_CD8_alpha; Immunoglobulin (Ig) like domain of CD8 alpha chain

smart00410
Location:38 → 142

IG_like; Immunoglobulin like
NM_009857.1 → NP_033987.1 T-cell surface glycoprotein CD8 alpha chain isoform 2 precursor

See identical proteins and their annotated locations for NP_033987.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an exon in the coding region resulting in a frameshift, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 1.

Source sequence(s)

AC160090

Consensus CDS

CCDS51806.1

UniProtKB/TrEMBL

Q8C2L1, Q8CAX3

Related

ENSMUSP00000131873.2, ENSMUST00000172321.3

Conserved Domains (2) summary

cd05720
Location:41 → 147

Ig_CD8_alpha; Immunoglobulin (Ig) like domain of CD8 alpha chain

smart00410
Location:38 → 142

IG_like; Immunoglobulin like