Eb1 Eb1 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Eb1provided by FlyBase
Official Full Name: Eb1provided by FlyBase
Primary source: FLYBASE:FBgn0027066
Locus tag: Dmel_CG3265
See related: AllianceGenome:FB:FBgn0027066
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-EST:Liang-2.33; anon-EST:Liang-2.52; BcDNA.LD08743; BcDNA:LD08743; CG3265; clone 2.33; clone 2.52; dEB-1; dEb1; dEB1; Dm EB1; dmEB1; DmEB1; Dmel\CG3265; EB-1; eb1; EB1; l(2)04524; l(2)4524; S(DmcycE[JP])2.5
Summary: Enables myosin binding activity. Involved in several processes, including melanotic encapsulation of foreign target; microtubule cytoskeleton organization; and pseudocleavage involved in syncytial blastoderm formation. Located in axonal growth cone; cytoplasm; and microtubule. Colocalizes with cytoplasmic microtubule; microtubule plus-end; and spindle microtubule. Is expressed in several structures, including adult head; embryonic/larval dorsal vessel; embryonic/larval optic lobe; female germline cell; and larval muscle cell. Human ortholog(s) of this gene implicated in congenital symmetric circumferential skin creases 2. Orthologous to several human genes including MAPRE1 (microtubule associated protein RP/EB family member 1) and MAPRE3 (microtubule associated protein RP/EB family member 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 42C8-42C8; 2-55 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (6749227..6756300)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (2636732..2643805)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Validation and classification of RNA binding proteins identified by mRNA interactome capture.
Title: Validation and classification of RNA binding proteins identified by mRNA interactome capture.
NOD produces congression forces by microtubule plus end-directed motility and tip-tracking on polymerizing microtubule plus ends via association with EB1.
Title: NOD is a plus end-directed motor that binds EB1 via a new microtubule tip localization sequence.
The authors that the cytokine receptor homolog Dome, acting downstream of the niche-derived ligand Upd, directly binds to the microtubule-binding protein Eb1 to regulate spindle orientation in Drosophila male germline stem cells (GSCs).
Title: Cytokine receptor-Eb1 interaction couples cell polarity and fate during asymmetric cell division.
tip-tracking of kinesin-14 occurs through several concurrent mechanisms: binding of kinesin only to EB1 located at the MT end, interaction of the kinesin molecules with a composite site formed by EB1 and the MT tip, and probably surface diffusion of the tail along MT.
Title: Insights into the process of EB1-dependent tip-tracking of kinesin-14 Ncd. The role of the microtubule.
Drosophila melanogaster larval muscles, exhibiting both elastic features contributed by the stretching capacity of MSP300 (nesprin) and rigidity provided by a perinuclear network of microtubules stabilized by Shot (spectraplakin) and EB1.
Title: Nesprin provides elastic properties to muscle nuclei by cooperating with spectraplakin and EB1.
Kank as an EB1 interactor that predominantly localises to embryonic attachment sites between muscle and tendon cells
Title: Kank Is an EB1 interacting protein that localises to muscle-tendon attachment sites in Drosophila.
Aptamers binding to human EB1 and EB3, which have sequence requirements similar to but distinct from each other and from Drosophila EB1, were identified.
Title: Peptide aptamers define distinct EB1- and EB3-binding motifs and interfere with microtubule dynamics.
In Drosophila melanogaster S2 cells, Dynamic microtubules (MT) tip-localizing protein EB1, has been identified as being critical for accelerating MT growth and promoting catastrophe events, thus resulting in the formation of dynamic MTs.
Title: Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin.
Binding of shot protein to EB1 requires SxIP motifs in Shot's carboxy-terminal tail (Ctail). Mutations of these motifs abolish Shot functions in axonal growth.
Title: Spectraplakins promote microtubule-mediated axonal growth by functioning as structural microtubule-associated proteins and EB1-dependent +TIPs (tip interacting proteins).
Data show that kinesin-2, and the +TIPS EB1 and APC, are required for uniform dendrite microtubule polarity.
Title: Directed microtubule growth, +TIPs, and kinesin-2 are required for uniform microtubule polarity in dendrites.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule binding	TAS Traceable Author Statement more info	PubMed
enables microtubule plus-end binding	IBA Inferred from Biological aspect of Ancestor more info
enables myosin binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in chromosome segregation	IDA Inferred from Direct Assay more info	PubMed
involved_in establishment of mitotic spindle localization	TAS Traceable Author Statement more info	PubMed
involved_in establishment or maintenance of microtubule cytoskeleton polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in flight behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in melanotic encapsulation of foreign target	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule cytoskeleton organization	IDA Inferred from Direct Assay more info	PubMed
involved_in microtubule-based process	TAS Traceable Author Statement more info	PubMed
involved_in mitotic spindle elongation	IDA Inferred from Direct Assay more info	PubMed
involved_in mitotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle organization	TAS Traceable Author Statement more info	PubMed
involved_in protein localization to microtubule	IBA Inferred from Biological aspect of Ancestor more info
involved_in pseudocleavage involved in syncytial blastoderm formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of microtubule polymerization or depolymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in sensory organ development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sensory perception of sound	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spindle assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in spindle assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in wound healing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in axonal growth cone	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with cytoplasmic microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule	IDA Inferred from Direct Assay more info	PubMed
part_of microtubule associated complex	TAS Traceable Author Statement more info	PubMed
is_active_in microtubule organizing center	IBA Inferred from Biological aspect of Ancestor more info
colocalizes_with microtubule plus-end	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with spindle microtubule	IDA Inferred from Direct Assay more info	PubMed
is_active_in spindle midzone	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Eb1

Names: +TIP End-Binding 1; CG3265-PA; CG3265-PB; CG3265-PD; CG3265-PE; CG3265-PF; CG3265-PG; Eb1-PA; Eb1-PB; Eb1-PD; Eb1-PE; Eb1-PF; Eb1-PG; End-Binding 1; MT-plus-end-binding protein; microtubule plus-tip protein EB1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

6749227..6756300

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_165487.4 → NP_724495.2 Eb1, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724495.2

Status: REVIEWED

UniProtKB/TrEMBL

A1Z6P3, D0Z742, Q6NNE2

Conserved Domains (3) summary

COG5217
Location:16 → 293

BIM1; Microtubule-binding protein involved in cell cycle control [Cell division and chromosome partitioning / Cytoskeleton]

pfam00307
Location:16 → 114

CH; Calponin homology (CH) domain

pfam03271
Location:241 → 279

EB1; EB1-like C-terminal motif
NM_136389.5 → NP_610233.1 Eb1, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_610233.1

Status: REVIEWED

UniProtKB/TrEMBL

D3DMK3, Q7JZD3

Related

FBpp0085461

Conserved Domains (2) summary

pfam00307
Location:16 → 114

CH; Calponin homology (CH) domain

pfam03271
Location:235 → 273

EB1; EB1-like C-terminal motif
NM_206030.3 → NP_995752.1 Eb1, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_995752.1

Status: REVIEWED

UniProtKB/TrEMBL

D3DMK3, Q9XZ57

Conserved Domains (2) summary

pfam00307
Location:16 → 114

CH; Calponin homology (CH) domain

pfam03271
Location:234 → 272

EB1; EB1-like C-terminal motif
NM_165489.3 → NP_724497.1 Eb1, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724497.1

Status: REVIEWED

UniProtKB/TrEMBL

D3DMK3, Q7JZD3

Related

FBpp0085463

Conserved Domains (2) summary

pfam00307
Location:16 → 114

CH; Calponin homology (CH) domain

pfam03271
Location:235 → 273

EB1; EB1-like C-terminal motif
NM_001273805.1 → NP_001260734.1 Eb1, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260734.1

Status: REVIEWED

UniProtKB/TrEMBL

D3DMK3, Q7JZD3

Conserved Domains (2) summary

pfam00307
Location:16 → 114

CH; Calponin homology (CH) domain

pfam03271
Location:235 → 273

EB1; EB1-like C-terminal motif
NM_165488.3 → NP_724496.1 Eb1, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724496.1

Status: REVIEWED

UniProtKB/TrEMBL

D3DMK3, Q7JZD3

Related

FBpp0085464

Conserved Domains (2) summary

pfam00307
Location:16 → 114

CH; Calponin homology (CH) domain

pfam03271
Location:235 → 273

EB1; EB1-like C-terminal motif