VPS33 tethering complex ATP-binding subunit VPS33 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: VPS33
Official Full Name: tethering complex ATP-binding subunit VPS33
Primary source: SGD:S000004388
Locus tag: YLR396C
See related: AllianceGenome:SGD:S000004388; FungiDB:YLR396C; VEuPathDB:YLR396C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CLS14; MET27; PEP14; SLP1; VAM5; VPL25; VPT33
Summary: Enables ATP binding activity. Contributes to phosphatidylinositol binding activity. Involved in several processes, including piecemeal microautophagy of the nucleus; vacuolar transport; and vacuole fusion. Located in cytosol and fungal-type vacuole membrane. Part of CORVET complex and HOPS complex. Human ortholog(s) of this gene implicated in arthrogryposis multiplex congenita; arthrogryposis, renal dysfunction, and cholestasis 1; cholestasis; kidney disease; and progressive familial intrahepatic cholestasis. Orthologous to several human genes including VPS33A (VPS33A core subunit of CORVET and HOPS complexes). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See VPS33 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (910235..912310, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Thus, Vps33/HOPS promotes productive SNARE assembly in the presence of otherwise inhibitory Sec17.
Title: Sec17 (α-SNAP) and an SM-tethering complex regulate the outcome of SNARE zippering in vitro and in vivo.
Homotypic fusion and protein-sorting/class C vacuole protein-sorting (HOPS/class C Vps) complex can tether low-curvature membranes, that is, liposomes with a diameter of 100 nm, only when the yeast vacuolar Ypt7p is present in both tethered membranes.
Title: The HOPS/class C Vps complex tethers membranes by binding to one Rab GTPase in each apposed membrane.
Sec17 directly promotes selective loading of Sly1 and Vps33 onto cognate SNARE complexes
Title: SM proteins Sly1 and Vps33 co-assemble with Sec17 and SNARE complexes to oppose SNARE disassembly by Sec18.
Vps33 appears to catalyze SNARE complex assembly through specific SNARE motif recognition.
Title: A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly.
Data show that the binding sites for vacuolar SNAREs and the SNARE Vam3 Habc domain are located in the large head of the HOPS complex, where vesicular transport proteins Vps16 and Vps33 have been identified.
Title: The Habc domain of the SNARE Vam3 interacts with the HOPS tethering complex to facilitate vacuole fusion.
we identify missense mutations of Vps33 that produce a novel set of defects, including cargo missorting and the loss of Vps33-HOPS association
Title: Sec1/Munc18 protein Vps33 binds to SNARE domains and the quaternary SNARE complex.
contribution of Vps33 to hemifusion and pore opening between vacuoles. Mutations in Vps33 that weaken its interactions with the SNARE complex allowed normal trans-SNARE pairing and lipid mixing but retarded content mixing.
Title: The SM protein Vps33 and the t-SNARE H(abc) domain promote fusion pore opening.
Vps33p functions at multiple trafficking steps and is not limited to action at the vacuolar membrane
Title: The Sec1/Munc18 protein, Vps33p, functions at the endosome and the vacuole of Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to phosphatidylinositol binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi to vacuole transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in piecemeal microautophagy of the nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein targeting to vacuole	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of SNARE complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in vacuole fusion, non-autophagic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vacuole organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle docking	IGI Inferred from Genetic Interaction more info	PubMed
involved_in vesicle fusion with vacuole	IGI Inferred from Genetic Interaction more info	PubMed
involved_in vesicle tethering	IDA Inferred from Direct Assay more info	PubMed
involved_in vesicle-mediated transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in vesicle-mediated transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of CORVET complex	IBA Inferred from Biological aspect of Ancestor more info
part_of CORVET complex	IDA Inferred from Direct Assay more info	PubMed
part_of HOPS complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in fungal-type vacuole	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole membrane	IPI Inferred from Physical Interaction more info	PubMed
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: tethering complex ATP-binding subunit VPS33

NP_013500.3

ATP-binding protein that is a subunit of the HOPS and CORVET complexes; essential for protein sorting, vesicle docking, and fusion at the vacuole; binds to SNARE domains

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

910235..912310 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182284.3 → NP_013500.3 TPA: tethering complex ATP-binding subunit VPS33 [Saccharomyces cerevisiae S288C]

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZ31, P20795

UniProtKB/TrEMBL

A7A1S1, B3RHR1, G2WJL1, N1P1U4

Conserved Domains (1) summary

COG5158
Location:14 → 651

SEC1; Proteins involved in synaptic transmission and general secretion, Sec1 family [Intracellular trafficking and secretion]