SSH1 slingshot protein phosphatase 1 [Homo sapiens (human)] - Gene

Summary

Official Symbol: SSH1provided by HGNC
Official Full Name: slingshot protein phosphatase 1provided by HGNC
Primary source: HGNC:HGNC:30579
See related: Ensembl:ENSG00000084112 MIM:606778; AllianceGenome:HGNC:30579
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: SSH1L
Summary: The protein encoded by this gene belongs to the slingshot homolog (SSH) family of phosphatases, which regulate actin filament dynamics. The SSH proteins dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Cofilin is inactivated by kinases such as LIM domain kinase-1 (LIMK1), which may also be dephosphorylated and inactivated by SSH proteins. The SSH family thus appears to play a role in actin dynamics by reactivating cofilin proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
Expression: Ubiquitous expression in gall bladder (RPKM 6.2), endometrium (RPKM 5.8) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 12q24.11

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	12	NC_000012.12 (108778191..108857583, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	12	NC_060936.1 (108752810..108832275, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	12	NC_000012.11 (109171967..109251359, complement)

Chromosome 12 - NC_000012.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dysregulated expression of slingshot protein phosphatase 1 (SSH1) disrupts circadian rhythm and WNT signaling associated to hepatocellular carcinoma pathogenesis.
Title: Dysregulated expression of slingshot protein phosphatase 1 (SSH1) disrupts circadian rhythm and WNT signaling associated to hepatocellular carcinoma pathogenesis.
Slingshot homolog-1-mediated Nrf2 sequestration tips the balance from neuroprotection to neurodegeneration in Alzheimer's disease.
Title: Slingshot homolog-1-mediated Nrf2 sequestration tips the balance from neuroprotection to neurodegeneration in Alzheimer's disease.
SSH1 promotes progression of intrahepatic cholangiocarcinoma via p38 MAPK-CXCL8 axis.
Title: SSH1 promotes progression of intrahepatic cholangiocarcinoma via p38 MAPK-CXCL8 axis.
Slingshot homolog-1 amplifies mitochondrial abnormalities by distinctly impairing health and clearance of mitochondria.
Title: Slingshot homolog-1 amplifies mitochondrial abnormalities by distinctly impairing health and clearance of mitochondria.
Role of SSH1 in colorectal cancer prognosis and tumor progression.
Title: Role of SSH1 in colorectal cancer prognosis and tumor progression.
genetic variants at the genes SSH1 and ST8SIA1 were related to the genetic predisposition to early-onset periodontitis that is in part triggered by smoking
Title: Smoking Modifies the Genetic Risk for Early-Onset Periodontitis.
SSH1 binds to gelsolin via actin filaments in the cytosolic fraction. Gelsolin promoted solubilization of actin filaments and SSH1 in cell-free assays and in cultured cells.
Title: Activation of cytosolic Slingshot-1 phosphatase by gelsolin-generated soluble actin filaments.
the N-terminal PH-like domain plays a critical role in F-actin binding and F-actin-mediated activation of the cofilin-phosphatase activity of SSH1
Title: A pleckstrin homology-like domain is critical for F-actin binding and cofilin-phosphatase activity of Slingshot-1.
Results reveal that SSH1L is upregulated in a subset of PCs and that the SSH1L/cofilin-1 signal pathway is associated positively in PC with cell migration.
Title: Cofilin-phosphatase slingshot-1L (SSH1L) is over-expressed in pancreatic cancer (PC) and contributes to tumor cell migration.
Authors show that viral penetration-induced calcium release facilitated herpes simplex virus type 1 intracellular trafficking through activating slingshot 1 (SSH), a phosphatase regulating actin filament dynamics.
Title: Calcium-signal facilitates herpes simplex virus type 1 nuclear transport through slingshot 1 and calpain-1 activation.

Submit: New GeneRIF Correction See all GeneRIFs (33)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH65932 (e-PCR)
- UniSTS:71706

24-1443 (e-PCR)
- UniSTS:240676

27-81 (e-PCR)
- UniSTS:240683

D8S2279 (e-PCR)
- UniSTS:473907

RH45532 (e-PCR)
- UniSTS:33617

STS-AA010350 (e-PCR)
- UniSTS:33771

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables MAP kinase serine/threonine phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables actin binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables calmodulin-dependent protein phosphatase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS140 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXY142 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables myosin phosphatase activity	IEA Inferred from Electronic Annotation more info
enables non-membrane spanning protein tyrosine phosphatase activity	IEA Inferred from Electronic Annotation more info
enables phosphoprotein phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphoprotein phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphoprotein phosphatase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein tyrosine phosphatase activity, metal-dependent	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cellular response to ATP	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in excitatory chemical synaptic transmission	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of actin filament polymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of AMPA glutamate receptor clustering	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of excitatory postsynaptic potential	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of synaptic plasticity	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of vascular associated smooth muscle cell migration	IEA Inferred from Electronic Annotation more info
involved_in protein dephosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of protein metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cleavage furrow	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in growth cone	IEA Inferred from Electronic Annotation more info
located_in lamellipodium	IEA Inferred from Electronic Annotation more info
located_in midbody	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein phosphatase Slingshot homolog 1

Names: SSH-like protein 1; hSSH-1L; slingshot homolog 1

NP_001154802.1

EC 3.1.3.16

EC 3.1.3.48

NP_001154803.1

EC 3.1.3.16

EC 3.1.3.48

NP_061857.3

EC 3.1.3.16

EC 3.1.3.48

XP_005269041.1

EC 3.1.3.16

EC 3.1.3.48

XP_005269042.1

EC 3.1.3.16

EC 3.1.3.48

XP_011536799.1

EC 3.1.3.16

EC 3.1.3.48

XP_011536801.1

EC 3.1.3.16

EC 3.1.3.48

XP_011536802.1

EC 3.1.3.16

EC 3.1.3.48

XP_011536803.1

EC 3.1.3.16

EC 3.1.3.48

XP_016874980.1

EC 3.1.3.16

EC 3.1.3.48

XP_047284977.1

EC 3.1.3.16

EC 3.1.3.48

XP_047284978.1

EC 3.1.3.16

EC 3.1.3.48

XP_047284979.1

EC 3.1.3.16

EC 3.1.3.48

XP_047284980.1

EC 3.1.3.16

EC 3.1.3.48

XP_047284981.1

EC 3.1.3.16

EC 3.1.3.48

XP_047284982.1

EC 3.1.3.16

EC 3.1.3.48

XP_054228292.1

EC 3.1.3.16

EC 3.1.3.48

XP_054228293.1

EC 3.1.3.16

EC 3.1.3.48

XP_054228294.1

EC 3.1.3.16

EC 3.1.3.48

XP_054228295.1

EC 3.1.3.16

EC 3.1.3.48

XP_054228296.1

EC 3.1.3.16

EC 3.1.3.48

XP_054228297.1

EC 3.1.3.16

EC 3.1.3.48

XP_054228298.1

EC 3.1.3.16

EC 3.1.3.48

XP_054228299.1

EC 3.1.3.16

EC 3.1.3.48

XP_054228300.1

EC 3.1.3.16

EC 3.1.3.48

XP_054228301.1

EC 3.1.3.16

EC 3.1.3.48

XP_054228302.1

EC 3.1.3.16

EC 3.1.3.48

XP_054228303.1

EC 3.1.3.16

EC 3.1.3.48

XP_054228304.1

EC 3.1.3.16

EC 3.1.3.48

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_029846.2 RefSeqGene

Range

5000..84392

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001161330.2 → NP_001154802.1 protein phosphatase Slingshot homolog 1 isoform 2

See identical proteins and their annotated locations for NP_001154802.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs at the 3' end compared to variant 1. This results in a shorter isoform (2, also known as SSH-1S) with a distinct C-terminus compared to isoform 1.

Source sequence(s)

AB072356, BP363810

Consensus CDS

CCDS55882.1

UniProtKB/Swiss-Prot

Q8WYL5

Related

ENSP00000448824.1, ENST00000551165.5

Conserved Domains (3) summary

cd11652
Location:3 → 237

SSH-N; N-terminal domain conserved in slingshot (SSH) phosphatases

cd00127
Location:308 → 444

DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...

pfam08766
Location:252 → 303

DEK_C; DEK C terminal domain
NM_001161331.1 → NP_001154803.1 protein phosphatase Slingshot homolog 1 isoform 3

See identical proteins and their annotated locations for NP_001154803.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) contains an alternative 5' terminal exon, and differs at the 3' end compared to variant 1. This results in a shorter isoform (3) with distinct N- and C-termini compared to isoform 1.

Source sequence(s)

AB072356, AK095421

Consensus CDS

CCDS53825.1

UniProtKB/Swiss-Prot

Q8WYL5

Related

ENSP00000326107.5, ENST00000326470.9

Conserved Domains (3) summary

cd11652
Location:41 → 248

SSH-N; N-terminal domain conserved in slingshot (SSH) phosphatases

cd00127
Location:319 → 455

DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...

pfam08766
Location:263 → 314

DEK_C; DEK C terminal domain
NM_018984.4 → NP_061857.3 protein phosphatase Slingshot homolog 1 isoform 1

See identical proteins and their annotated locations for NP_061857.3

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as SSH-1L). This isoform has been shown to be enzymatically active (PMID:11832213).

Source sequence(s)

AB072355, AC087893, AI621348, BC020243, BP363810

Consensus CDS

CCDS9121.1

UniProtKB/Swiss-Prot

Q6P6C0, Q8N9A7, Q8WYL3, Q8WYL4, Q8WYL5, Q9P2P8

Related

ENSP00000315713.5, ENST00000326495.10

Conserved Domains (3) summary

cd11652
Location:3 → 237

SSH-N; N-terminal domain conserved in slingshot (SSH) phosphatases

cd00127
Location:308 → 444

DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...

pfam08766
Location:252 → 303

DEK_C; DEK C terminal domain

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000012.12 Reference GRCh38.p14 Primary Assembly

Range

108778191..108857583 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_047429022.1 → XP_047284978.1 protein phosphatase Slingshot homolog 1 isoform X4
XM_005268985.3 → XP_005269042.1 protein phosphatase Slingshot homolog 1 isoform X7

See identical proteins and their annotated locations for XP_005269042.1

Conserved Domains (3) summary

cd11652
Location:1 → 157

SSH-N; N-terminal domain conserved in slingshot (SSH) phosphatases

cd00127
Location:228 → 364

DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...

pfam08766
Location:172 → 223

DEK_C; DEK C terminal domain
XM_047429023.1 → XP_047284979.1 protein phosphatase Slingshot homolog 1 isoform X6
XM_047429021.1 → XP_047284977.1 protein phosphatase Slingshot homolog 1 isoform X4
XM_005268984.5 → XP_005269041.1 protein phosphatase Slingshot homolog 1 isoform X2

Conserved Domains (3) summary

cd11652
Location:11 → 237

SSH-N; N-terminal domain conserved in slingshot (SSH) phosphatases

cd00127
Location:308 → 444

DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...

pfam08766
Location:252 → 303

DEK_C; DEK C terminal domain
XM_011538499.2 → XP_011536801.1 protein phosphatase Slingshot homolog 1 isoform X5

Conserved Domains (3) summary

cd11652
Location:41 → 248

SSH-N; N-terminal domain conserved in slingshot (SSH) phosphatases

pfam08766
Location:263 → 314

DEK_C; DEK C terminal domain

cl21483
Location:320 → 401

PTPc; Protein tyrosine phosphatases (PTP) catalyze the dephosphorylation of phosphotyrosine peptides; they regulate phosphotyrosine levels in signal transduction pathways. The depth of the active site cleft renders the enzyme specific for phosphorylated Tyr ...
XM_011538497.2 → XP_011536799.1 protein phosphatase Slingshot homolog 1 isoform X1

Conserved Domains (3) summary

cd11652
Location:41 → 248

SSH-N; N-terminal domain conserved in slingshot (SSH) phosphatases

cd00127
Location:319 → 455

DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...

pfam08766
Location:263 → 314

DEK_C; DEK C terminal domain
XM_047429024.1 → XP_047284980.1 protein phosphatase Slingshot homolog 1 isoform X8
XM_017019491.3 → XP_016874980.1 protein phosphatase Slingshot homolog 1 isoform X3
XM_011538501.4 → XP_011536803.1 protein phosphatase Slingshot homolog 1 isoform X7

See identical proteins and their annotated locations for XP_011536803.1

Conserved Domains (3) summary

cd11652
Location:1 → 157

SSH-N; N-terminal domain conserved in slingshot (SSH) phosphatases

cd00127
Location:228 → 364

DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...

pfam08766
Location:172 → 223

DEK_C; DEK C terminal domain
XM_011538500.4 → XP_011536802.1 protein phosphatase Slingshot homolog 1 isoform X7

See identical proteins and their annotated locations for XP_011536802.1

Conserved Domains (3) summary

cd11652
Location:1 → 157

SSH-N; N-terminal domain conserved in slingshot (SSH) phosphatases

cd00127
Location:228 → 364

DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...

pfam08766
Location:172 → 223

DEK_C; DEK C terminal domain
XM_047429025.1 → XP_047284981.1 protein phosphatase Slingshot homolog 1 isoform X9
XM_047429026.1 → XP_047284982.1 protein phosphatase Slingshot homolog 1 isoform X10

Alternate T2T-CHM13v2.0

Genomic

NC_060936.1 Alternate T2T-CHM13v2.0

Range

108752810..108832275 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054372321.1 → XP_054228296.1 protein phosphatase Slingshot homolog 1 isoform X4
XM_054372326.1 → XP_054228301.1 protein phosphatase Slingshot homolog 1 isoform X7
XM_054372323.1 → XP_054228298.1 protein phosphatase Slingshot homolog 1 isoform X6
XM_054372320.1 → XP_054228295.1 protein phosphatase Slingshot homolog 1 isoform X4
XM_054372318.1 → XP_054228293.1 protein phosphatase Slingshot homolog 1 isoform X2
XM_054372322.1 → XP_054228297.1 protein phosphatase Slingshot homolog 1 isoform X5
XM_054372317.1 → XP_054228292.1 protein phosphatase Slingshot homolog 1 isoform X1
XM_054372327.1 → XP_054228302.1 protein phosphatase Slingshot homolog 1 isoform X8
XM_054372319.1 → XP_054228294.1 protein phosphatase Slingshot homolog 1 isoform X3
XM_054372325.1 → XP_054228300.1 protein phosphatase Slingshot homolog 1 isoform X7
XM_054372324.1 → XP_054228299.1 protein phosphatase Slingshot homolog 1 isoform X7
XM_054372328.1 → XP_054228303.1 protein phosphatase Slingshot homolog 1 isoform X9
XM_054372329.1 → XP_054228304.1 protein phosphatase Slingshot homolog 1 isoform X10