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    NT5E 5'-nucleotidase ecto [ Homo sapiens (human) ]

    Gene ID: 4907, updated on 10-Dec-2024

    Summary

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    Official Symbol
    NT5Eprovided by HGNC
    Official Full Name
    5'-nucleotidase ectoprovided by HGNC
    Primary source
    HGNC:HGNC:8021
    See related
    Ensembl:ENSG00000135318 MIM:129190; AllianceGenome:HGNC:8021
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
    Summary
    The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
    Expression
    Ubiquitous expression in endometrium (RPKM 30.0), ovary (RPKM 19.6) and 22 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See NT5E in Genome Data Viewer
    Location:
    6q14.3
    Exon count:
    9
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 6 NC_000006.12 (85450083..85495784)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 6 NC_060930.1 (86666882..86712587)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 6 NC_000006.11 (86159801..86205502)

    Chromosome 6 - NC_000006.12Genomic Context describing neighboring genes Neighboring gene TPT1 pseudogene 6 Neighboring gene deoxyuridine triphosphatase pseudogene 5 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17366 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24787 Neighboring gene ReSE screen-validated silencer GRCh37_chr6:86210956-86211197 Neighboring gene sorting nexin 14 Neighboring gene Sharpr-MPRA regulatory region 13581 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr6:86262889-86263390 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr6:86263391-86263890 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr6:86302067-86302652 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:86302653-86303236 Neighboring gene H3K27ac hESC enhancer GRCh37_chr6:86303237-86303820 Neighboring gene synaptotagmin binding cytoplasmic RNA interacting protein Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17368 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17369 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17370

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. CD73 is associated with poor prognosis in high-grade serous ovarian cancer. Turcotte M, et al. Cancer Res, 2015 Nov 1. PMID 26363007
    2. Ecto-5'-nucleotidase (CD73) is a biomarker for clear cell renal carcinoma stem-like cells. Song L, et al. Oncotarget, 2017 May 9. PMID 28404888, Free PMC Article
    3. CD73 promotes proliferation and migration of human cervical cancer cells independent of its enzyme activity. Gao ZW, et al. BMC Cancer, 2017 Feb 15. PMID 28202050, Free PMC Article
    4. Down-regulation of CD73 on B cells of patients with viremic HIV correlates with B cell activation and disease progression. Kim ES, et al. J Leukoc Biol, 2017 May. PMID 28193736
    5. Polymorphism in exon 6 of the human NT5E gene is associated with aortic valve calcification. Kochan Z, et al. Nucleosides Nucleotides Nucleic Acids, 2016 Dec. PMID 27906615

    See all (309) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. CD73 promotes non-small cell lung cancer metastasis by regulating Axl signaling independent of GAS6.
      Title: CD73 promotes non-small cell lung cancer metastasis by regulating Axl signaling independent of GAS6.
    2. High levels of soluble CD73 unveil resistance to BRAF inhibitors in melanoma cells.
      Title: High levels of soluble CD73 unveil resistance to BRAF inhibitors in melanoma cells.
    3. Regulation of CD73 on NAD metabolism: Unravelling the interplay between tumour immunity and tumour metabolism.
      Title: Regulation of CD73 on NAD metabolism: Unravelling the interplay between tumour immunity and tumour metabolism.
    4. The immunomodulatory ballet of tumour-derived extracellular vesicles and neutrophils orchestrating the dynamic CD73/PD-L1 pathway in cancer.
      Title: The immunomodulatory ballet of tumour-derived extracellular vesicles and neutrophils orchestrating the dynamic CD73/PD-L1 pathway in cancer.
    5. ENTPD1 (CD39) and NT5E (CD73) expression in human glioblastoma: an in silico analysis.
      Title: ENTPD1 (CD39) and NT5E (CD73) expression in human glioblastoma: an in silico analysis.
    6. CD73 as a T cell dysfunction marker predicting cardiovascular and infection events in patients undergoing hemodialysis.
      Title: CD73 as a T cell dysfunction marker predicting cardiovascular and infection events in patients undergoing hemodialysis.
    7. Altered CD39 and CD73 Expression in Rheumatoid Arthritis: Implications for Disease Activity and Treatment Response.
      Title: Altered CD39 and CD73 Expression in Rheumatoid Arthritis: Implications for Disease Activity and Treatment Response.
    8. Human plasmacytoid dendritic cells express the functional purinergic halo (CD39/CD73).
      Title: Human plasmacytoid dendritic cells express the functional purinergic halo (CD39/CD73).
    9. CD73 contributes to the pathogenesis of fusion-negative rhabdomyosarcoma through the purinergic signaling pathway.
      Title: CD73 contributes to the pathogenesis of fusion-negative rhabdomyosarcoma through the purinergic signaling pathway.
    10. The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
      Title: The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
    Submit: New GeneRIF Correction See all GeneRIFs (234)

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Associated conditions

    Description Tests
    Hereditary arterial and articular multiple calcification syndrome
    MedGen: C1859372 OMIM: 211800 GeneReviews: Not available
    		not available

    EBI GWAS Catalog

    Description
    An atlas of genetic influences on human blood metabolites.
    EBI GWAS Catalog
    Human metabolic individuality in biomedical and pharmaceutical research.
    EBI GWAS Catalog

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    HIV-1 interactions

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    Protein interactions

    Protein Gene Interaction Pubs
    Vif vif HIV-1 Vif upregulates the expression of 5'-nucleotidase, ecto (NT5E, CD73) in Vif-expression T cells PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables 5'-deoxynucleotidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 5'-nucleotidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 5'-nucleotidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 5'-nucleotidase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables GMP 5'-nucleotidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables IMP 5'-nucleotidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables XMP 5'-nucleosidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables thymidylate 5'-phosphatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in ADP catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in AMP catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ATP metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA metabolic process TAS
    Traceable Author Statement
    more info
    		 PubMed 
    involved_in adenosine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in calcium ion homeostasis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in inhibition of non-skeletal tissue mineralization IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in leukocyte cell-cell adhesion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in negative regulation of inflammatory response IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to ATP IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cell surface HDA PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		  
    located_in external side of plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in extracellular exosome HDA PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		  
    located_in plasma membrane TAS
    Traceable Author Statement
    more info
    		  

    General protein information

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    Preferred Names
    5'-nucleotidase
    Names
    5'-NT
    5'-deoxynucleotidase
    IMP-specific 5'-nucleotidase
    Purine 5-Prime-Nucleotidase
    ecto-5'-nucleotidase
    thymidylate 5'-phosphatase
    NP_001191742.1
    NP_002517.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_028214.1 RefSeqGene

      Range
      5500..51201
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001204813.2 → NP_001191742.1  5'-nucleotidase isoform 2 preproprotein

      See identical proteins and their annotated locations for NP_001191742.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.
      Source sequence(s)
      AK075008, BC065937, BQ019358, DA574510, X55740
      Consensus CDS
      CCDS56439.1
      UniProtKB/TrEMBL
      B2RBH2
      Related
      ENSP00000358665.3, ENST00000369651.7
      Conserved Domains (3) summary
      COG0737
      Location:11 → 482
      UshA; 2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'- or 3'-nucleotidase, 5'-nucleotidase family [Nucleotide transport and metabolism, Defense mechanisms]
      cd07409
      Location:29 → 310
      MPP_CD73_N; CD73 ecto-5'-nucleotidase and related proteins, N-terminal metallophosphatase domain
      pfam02872
      Location:339 → 457
      5_nucleotid_C; 5'-nucleotidase, C-terminal domain

    2. NM_002526.4 → NP_002517.1  5'-nucleotidase isoform 1 preproprotein

      See identical proteins and their annotated locations for NP_002517.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AK075008, BC065937, BQ019358, DA574510, X55740
      Consensus CDS
      CCDS5002.1
      UniProtKB/Swiss-Prot
      B3KQI8, O75520, P21589, Q5W116
      UniProtKB/TrEMBL
      B2RBH2
      Related
      ENSP00000257770.3, ENST00000257770.8
      Conserved Domains (3) summary
      COG0737
      Location:11 → 532
      UshA; 2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'- or 3'-nucleotidase, 5'-nucleotidase family [Nucleotide transport and metabolism, Defense mechanisms]
      cd07409
      Location:29 → 310
      MPP_CD73_N; CD73 ecto-5'-nucleotidase and related proteins, N-terminal metallophosphatase domain
      pfam02872
      Location:339 → 507
      5_nucleotid_C; 5'-nucleotidase, C-terminal domain

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000006.12 Reference GRCh38.p14 Primary Assembly

      Range
      85450083..85495784
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060930.1 Alternate T2T-CHM13v2.0

      Range
      86666882..86712587
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P21589.1 GenPept UniProtKB/Swiss-Prot:P21589

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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