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    ORC2 origin recognition complex subunit 2 [ Homo sapiens (human) ]

    Gene ID: 4999, updated on 10-Dec-2024

    Summary

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    Official Symbol
    ORC2provided by HGNC
    Official Full Name
    origin recognition complex subunit 2provided by HGNC
    Primary source
    HGNC:HGNC:8488
    See related
    Ensembl:ENSG00000115942 MIM:601182; AllianceGenome:HGNC:8488
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    ORC2L
    Summary
    The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate. [provided by RefSeq, Oct 2010]
    Expression
    Ubiquitous expression in testis (RPKM 10.9), thyroid (RPKM 5.8) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See ORC2 in Genome Data Viewer
    Location:
    2q33.1
    Exon count:
    20
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 2 NC_000002.12 (200908977..200963660, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 2 NC_060926.1 (201392427..201447106, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (201773700..201828383, complement)

    Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene BRD4-independent group 4 enhancer GRCh37_chr2:201753436-201754635 Neighboring gene RNA, U6 small nuclear 312, pseudogene Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16968 Neighboring gene peptidylprolyl isomerase like 3 Neighboring gene NGG1 interacting factor 3 like 1 Neighboring gene RNA, U6 small nuclear 762, pseudogene Neighboring gene OCT4 hESC enhancer GRCh37_chr2:201799831-201800332 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16969 Neighboring gene uncharacterized LOC105373835 Neighboring gene ReSE screen-validated silencer GRCh37_chr2:201828394-201828792 Neighboring gene hyccin PI4KA lipid kinase complex subunit 2 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16970 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr2:201907391-201907902 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr2:201907903-201908415 Neighboring gene heterogeneous nuclear ribonucleoprotein A1 pseudogene 35 Neighboring gene ribosomal protein L23a pseudogene 30

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. Circ_ORC2 enhances the regulatory effect of miR-19a on its target gene PTEN to affect osteosarcoma cell growth. Li X, et al. Biochem Biophys Res Commun, 2019 Jul 5. PMID 31103262
    2. Human minichromosome maintenance proteins and human origin recognition complex 2 protein on chromatin. Ritzi M, et al. J Biol Chem, 1998 Sep 18. PMID 9733749
    3. SUMOylated ORC2 Recruits a Histone Demethylase to Regulate Centromeric Histone Modification and Genomic Stability. Huang C, et al. Cell Rep, 2016 Apr 5. PMID 27052177
    4. Dephosphorylation of Orc2 by protein phosphatase 1 promotes the binding of the origin recognition complex to chromatin. Lee KY, et al. Biochem Biophys Res Commun, 2014 Jun 13. PMID 24792176
    5. Protein phosphatase 1 dephosphorylates Orc2. Lee KY, et al. Biochem Biophys Res Commun, 2014 May 9. PMID 24732362

    See all (124) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteins.
      Title: A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteins.
    2. The circ_ORC2 binds with miR-19a and enhances its expression, thereby inhibiting downstream PTEN expression and activating Akt pathway to promote osteosarcoma cell growth and invasion.
      Title: Circ_ORC2 enhances the regulatory effect of miR-19a on its target gene PTEN to affect osteosarcoma cell growth.
    3. The authors have discovered that human cell lines in culture proliferate with intact chromosomal origins of replication after disruption of both alleles of ORC2 or of the ATPase subunit, ORC1.
      Title: Two subunits of human ORC are dispensable for DNA replication and proliferation.
    4. the ORC2 complex with E2 restricts viral replication in the maintenance phase of the viral replication program
      Title: The Replicative Consequences of Papillomavirus E2 Protein Binding to the Origin Replication Factor ORC2.
    5. ORC2 SUMOylation promotes recruitment of KDM5A to centromeres.
      Title: SUMOylated ORC2 Recruits a Histone Demethylase to Regulate Centromeric Histone Modification and Genomic Stability.
    6. Protein phosphatase 1 dephosphorylates Orc2 to promote the binding of ORC to chromatin and replication origins for the subsequent round of the cell cycle.
      Title: Dephosphorylation of Orc2 by protein phosphatase 1 promotes the binding of the origin recognition complex to chromatin.
    7. These results suggest that PP1 dephosphorylates Orc2 to promote the binding of ORC to chromatin.
      Title: Protein phosphatase 1 dephosphorylates Orc2.
    8. Gemcitabine resistance is dependent on Plk1-mediated phosphorylation of Orc2 and Hbo1.
      Title: Plk1 phosphorylation of orc2 and hbo1 contributes to gemcitabine resistance in pancreatic cancer.
    9. Orc2 protects ORCA from ubiquitin-mediated degradation in vivo.
      Title: Orc2 protects ORCA from ubiquitin-mediated degradation.
    10. The phosphorylation of ORC2 dissociates origin recognition complex (ORC) from chromatin and replication origins and inhibits binding of ORC to newly replicated DNA.
      Title: Phosphorylation of ORC2 protein dissociates origin recognition complex from chromatin and replication origins.
    Submit: New GeneRIF Correction See all GeneRIFs (17)

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables DNA replication origin binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA replication initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in negative regulation of transcription by RNA polymerase II TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in centrosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chromosome, telomeric region HDA PubMed 
    colocalizes_with chromosome, telomeric region IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in heterochromatin IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of inner kinetochore IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane HDA PubMed 
    part_of nuclear origin of replication recognition complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of nuclear origin of replication recognition complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of nuclear origin of replication recognition complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		  
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of origin recognition complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    origin recognition complex subunit 2
    Names
    origin recognition complex protein 2 homolog
    origin recognition complex, subunit 2 homolog

    NCBI Reference Sequences (RefSeq)

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    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_006190.5 → NP_006181.1  origin recognition complex subunit 2

      See identical proteins and their annotated locations for NP_006181.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes a functional protein.
      Source sequence(s)
      AC005037, AI033876, DA111542, U40268
      Consensus CDS
      CCDS2334.1
      UniProtKB/Swiss-Prot
      Q13204, Q13416, Q53TX5
      UniProtKB/TrEMBL
      B4DYU9
      Related
      ENSP00000234296.2, ENST00000234296.7
      Conserved Domains (1) summary
      pfam04084
      Location:256 → 565
      ORC2; Origin recognition complex subunit 2

    RNA

    1. NR_033915.2 RNA Sequence

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) retains an intron in the CDS, resulting in a premature stop codon, as compared to variant 1. The transcript is a nonsense-mediated mRNA decay candidate, and is probably not making a functional protein.
      Source sequence(s)
      AC005037, AI033876, AK302614, BP264347, DA545257, U40268

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000002.12 Reference GRCh38.p14 Primary Assembly

      Range
      200908977..200963660 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011511252.4 → XP_011509554.1  origin recognition complex subunit 2 isoform X1

      See identical proteins and their annotated locations for XP_011509554.1

      UniProtKB/TrEMBL
      B4DYU9
      Conserved Domains (1) summary
      pfam04084
      Location:276 → 582
      ORC2; Origin recognition complex subunit 2

    2. XM_006712555.5 → XP_006712618.1  origin recognition complex subunit 2 isoform X2

      See identical proteins and their annotated locations for XP_006712618.1

      UniProtKB/Swiss-Prot
      Q13204, Q13416, Q53TX5
      UniProtKB/TrEMBL
      B4DYU9
      Conserved Domains (1) summary
      pfam04084
      Location:256 → 565
      ORC2; Origin recognition complex subunit 2

    3. XM_047444569.1 → XP_047300525.1  origin recognition complex subunit 2 isoform X2

      UniProtKB/Swiss-Prot
      Q13204, Q13416, Q53TX5
      UniProtKB/TrEMBL
      B4DYU9

    4. XM_011511253.4 → XP_011509555.1  origin recognition complex subunit 2 isoform X1

      See identical proteins and their annotated locations for XP_011509555.1

      UniProtKB/TrEMBL
      B4DYU9
      Conserved Domains (1) summary
      pfam04084
      Location:276 → 582
      ORC2; Origin recognition complex subunit 2

    5. XM_047444568.1 → XP_047300524.1  origin recognition complex subunit 2 isoform X1

      UniProtKB/TrEMBL
      B4DYU9

    6. XM_047444572.1 → XP_047300528.1  origin recognition complex subunit 2 isoform X4

    7. XM_047444570.1 → XP_047300526.1  origin recognition complex subunit 2 isoform X3

    RNA

    1. XR_002959301.2 RNA Sequence

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060926.1 Alternate T2T-CHM13v2.0

      Range
      201392427..201447106 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054342304.1 → XP_054198279.1  origin recognition complex subunit 2 isoform X1

      UniProtKB/TrEMBL
      B4DYU9

    2. XM_054342307.1 → XP_054198282.1  origin recognition complex subunit 2 isoform X2

      UniProtKB/Swiss-Prot
      Q13204, Q13416, Q53TX5
      UniProtKB/TrEMBL
      B4DYU9

    3. XM_054342306.1 → XP_054198281.1  origin recognition complex subunit 2 isoform X1

      UniProtKB/TrEMBL
      B4DYU9

    4. XM_054342308.1 → XP_054198283.1  origin recognition complex subunit 2 isoform X2

      UniProtKB/Swiss-Prot
      Q13204, Q13416, Q53TX5
      UniProtKB/TrEMBL
      B4DYU9

    5. XM_054342305.1 → XP_054198280.1  origin recognition complex subunit 2 isoform X1

      UniProtKB/TrEMBL
      B4DYU9

    6. XM_054342310.1 → XP_054198285.1  origin recognition complex subunit 2 isoform X4

    7. XM_054342309.1 → XP_054198284.1  origin recognition complex subunit 2 isoform X3

    RNA

    1. XR_008486379.1 RNA Sequence

    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q13416.2 GenPept UniProtKB/Swiss-Prot:Q13416

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