Htr3a 5-hydroxytryptamine (serotonin) receptor 3A [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Htr3aprovided by MGI
Official Full Name: 5-hydroxytryptamine (serotonin) receptor 3Aprovided by MGI
Primary source: MGI:MGI:96282
See related: Ensembl:ENSMUSG00000032269 AllianceGenome:MGI:96282
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 5-HT3; 5-HT3A; 5-HT3R
Summary: Enables identical protein binding activity and ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential. Predicted to be involved in several processes, including chemical synaptic transmission; inorganic cation transmembrane transport; and serotonin receptor signaling pathway. Part of serotonin-activated cation-selective channel complex. Is expressed in several structures, including central nervous system; eye; gallbladder; genitourinary system; and gut. Human ortholog(s) of this gene implicated in alcohol dependence; cocaine dependence; heroin dependence; irritable bowel syndrome; and nicotine dependence. Orthologous to human HTR3A (5-hydroxytryptamine receptor 3A). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in colon adult (RPKM 3.9), adrenal adult (RPKM 3.2) and 14 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 A5.3; 9 26.53 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (48810513..48822451, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (48899213..48911152, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Upregulated NMDAR-mediated GABAergic transmission underlies autistic-like deficits in Htr3a knockout mice.
Title: Upregulated NMDAR-mediated GABAergic transmission underlies autistic-like deficits in Htr3a knockout mice.
High-resolution structures of multiple 5-HT3AR-setron complexes reveal a novel mechanism of competitive inhibition.
Title: High-resolution structures of multiple 5-HT(3A)R-setron complexes reveal a novel mechanism of competitive inhibition.
Asymmetric opening of the homopentameric 5-HT3A serotonin receptor in lipid bilayers.
Title: Asymmetric opening of the homopentameric 5-HT(3A) serotonin receptor in lipid bilayers.
Study performed multiple all-atom molecular dynamics simulations of the mouse homomeric 5-hydroxytryptamine 3A (5-HT3A) serotonin receptor for 15 to 20 mus to observe the dynamic binding and unbinding of 5-hydroxytryptamine (5-HT) (i.e., serotonin) to the binding pocket located on the extracellular domain and allosteric regulation of the transmembrane domain from synergistic 5-HT binding.
Title: Microsecond-timescale simulations suggest 5-HT-mediated preactivation of the 5-HT(3A) serotonin receptor.
5-HT3R signaling may enhance 5-HT release, possibly from EC cells intravascularly, or paracrine, resulting in increases in plasma 5-HT concentration, which in turn, enhances apoptotic activities induced by 5-FU
Title: Serotonin 3 receptor signaling regulates 5-fluorouracil-mediated apoptosis indirectly via TNF-α production by enhancing serotonin release from enterochromaffin cells.
four cryo-electron microscopy structures of the full-length mouse 5-HT3 receptor in complex with the anti-emetic drug tropisetron, with serotonin, and with serotonin and a positive allosteric modulator, at resolutions ranging from 3.2 A to 4.5 A
Title: Conformational transitions of the serotonin 5-HT(3) receptor.
two serotonin-bound cryoelectron microscopy structures of the full-length 5-HT3A receptor in distinct conformations at 3.32 A and 3.89 A resolution that reveal the mechanism underlying channel activation
Title: Cryo-EM reveals two distinct serotonin-bound conformations of full-length 5-HT(3A) receptor.
Results found that 5-HT3aR plays a role in lacrimal gland secretory functions and confirmed that extracellular Ca2+ entry participates in [Ca2+]i mobilization stimulated by 5-HT.
Title: Serotonin hormonally regulates lacrimal gland secretory function via the serotonin type 3a receptor.
Drd2 and Htr3a genes may play the key role in the synchronization of other genes of neurotransmitter systems in the ventral tegmental area of depressive male mice.
Title: [Altered Expression of Neurotransmitters Systems' Genes in the Ventral Tegmental Area of Depressive Male Mice: Data of RNA-Seq].
A triad of residues aligning to Thr-152, Glu-209, and Lys-211 in Htr3, appear to be involved in side-chain interactions near binding sites in Htr3a (subunit alpha) and muscle-type Chrna1. Data suggest that mutating Htr3a triad to that of Chrna1 increases binding affinity of nicotine to Htr3a. (Htr3 = 5-hydroxytryptamine/serotonin receptor; Chrna1 = cholinergic receptor nicotinic alpha polypeptide 1)
Title: A triad of residues is functionally transferrable between 5-HT(3) serotonin receptors and nicotinic acetylcholine receptors.

Submit: New GeneRIF Correction See all GeneRIFs (69)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (8)
Targeted (6) 1 citation

General gene information

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Markers

Htr3a (e-PCR), detects polymorphism
- UniSTS:143427

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables excitatory extracellular ligand-gated monoatomic ion channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential	IDA Inferred from Direct Assay more info	PubMed
enables ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed
enables ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential	NAS Non-traceable Author Statement more info	PubMed
enables serotonin binding	ISO Inferred from Sequence Orthology more info	PubMed
enables serotonin-gated monoatomic cation channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables serotonin-gated monoatomic cation channel activity	ISO Inferred from Sequence Orthology more info	PubMed
enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in chemical synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in inorganic cation transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in monoatomic ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of membrane potential	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of presynaptic membrane potential	IEA Inferred from Electronic Annotation more info
involved_in serotonin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in serotonin-gated cation-selective signaling pathway	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in axon	ISO Inferred from Sequence Orthology more info
located_in cleavage furrow	IEA Inferred from Electronic Annotation more info
located_in cleavage furrow	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic membrane	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic membrane	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic membrane	ISO Inferred from Sequence Orthology more info
part_of serotonin-activated cation-selective channel complex	IPI Inferred from Physical Interaction more info	PubMed
part_of serotonin-activated cation-selective channel complex	ISO Inferred from Sequence Orthology more info
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info
part_of transmembrane transporter complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: 5-hydroxytryptamine receptor 3A

Names: 5-hydroxytryptamine receptor 3; 5HT3 serotonin receptor; serotonin receptor 3A; serotonin-gated ion channel receptor

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001099644.1 → NP_001093114.1 5-hydroxytryptamine receptor 3A isoform 2 precursor

See identical proteins and their annotated locations for NP_001093114.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1.

Source sequence(s)

AF513221, BY275174, CJ237116

Consensus CDS

CCDS90559.1

UniProtKB/TrEMBL

B7ZMU3, Q8K1F4

Related

ENSMUSP00000150647.2, ENSMUST00000217289.2

Conserved Domains (3) summary

TIGR00860
Location:5 → 477

LIC; Cation transporter family protein

pfam02931
Location:41 → 247

Neur_chan_LBD; Neurotransmitter-gated ion-channel ligand binding domain

pfam02932
Location:254 → 473

Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region
NM_013561.2 → NP_038589.2 5-hydroxytryptamine receptor 3A isoform 1 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).

Source sequence(s)

AF513221, BY274793, CJ237116, M74425

Consensus CDS

CCDS52788.1

UniProtKB/Swiss-Prot

E9QLC0, P23979, Q61225, Q61226

UniProtKB/TrEMBL

B7ZMU3

Related

ENSMUSP00000003826.8, ENSMUST00000003826.8

Conserved Domains (1) summary

TIGR00860
Location:5 → 483

LIC; Cation transporter family protein

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

48810513..48822451 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011242398.4 → XP_011240700.1 5-hydroxytryptamine receptor 3A isoform X1

UniProtKB/TrEMBL

B7ZMU3

Conserved Domains (1) summary

TIGR00860
Location:5 → 468

LIC; Cation transporter family protein