Ndc80 NDC80 kinetochore complex component [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ndc80provided by MGI
Official Full Name: NDC80 kinetochore complex componentprovided by MGI
Primary source: MGI:MGI:1914302
See related: Ensembl:ENSMUSG00000024056 AllianceGenome:MGI:1914302
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: HEC; HEC1; Kntc2; 2610020P18Rik
Summary: Enables cyclin binding activity. Acts upstream of or within several processes, including kinetochore organization; meiosis I cell cycle process; and skeletal muscle satellite cell proliferation. Located in centrosome; cytoplasm; and kinetochore. Orthologous to human NDC80 (NDC80 kinetochore complex component). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in CNS E11.5 (RPKM 11.9), liver E14 (RPKM 11.3) and 9 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 E1.3; 17 41.87 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (71803095..71833852, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (71496100..71526857, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes.
Title: Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes.
These data strongly indicated that KNTC2 is a promising target for the treatment of HCC and that phosphorylated HH3 at serine 10 is one of the target engagement markers for KNTC2.
Title: Anti-tumor activity of KNTC2 siRNA in orthotopic tumor model mice of hepatocellular carcinoma.
Further investigation of the phosphoregulation of meiotic Kif4 revealed that Aurora Kinase and Cdk activity is critical for Kif4 kinetochore localization and interaction with Ndc80 and CENP-C. Finally, Kif4 protein but not gene expression was found to be upregulated with age, suggesting a role for this protein in the decline of oocyte quality with age
Title: Kif4 Is Essential for Mouse Oocyte Meiosis.
Hec1-dependent cyclin B2 stabilization during early metaphase is important for early-stage spindle assembly.
Title: Hec1-dependent cyclin B2 stabilization regulates the G2-M transition and early prometaphase in mouse oocytes.
Disruption of Ndc80 function by either siRNA injection or antibody injection resulted in severe chromosome misalignment, spindle disruption, and precocious polar body extrusion.
Title: Ndc80 regulates meiotic spindle organization, chromosome alignment, and cell cycle progression in mouse oocytes.
overexpression of Hec1 results in mitotic checkpoint hyperactivation that leads to aneuploidy sufficient to generate tumors
Title: Hec1 overexpression hyperactivates the mitotic checkpoint and induces tumor formation in vivo.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables cyclin binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinetochore adaptor activity	ISO Inferred from Sequence Orthology more info
enables kinetochore adaptor activity	ISS Inferred from Sequence or Structural Similarity more info
enables microtubule binding	ISO Inferred from Sequence Orthology more info
enables microtubule binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within G2/MI transition of meiotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in attachment of mitotic spindle microtubules to kinetochore	IBA Inferred from Biological aspect of Ancestor more info
involved_in attachment of mitotic spindle microtubules to kinetochore	ISO Inferred from Sequence Orthology more info
involved_in attachment of mitotic spindle microtubules to kinetochore	ISS Inferred from Sequence or Structural Similarity more info
involved_in attachment of spindle microtubules to kinetochore	ISO Inferred from Sequence Orthology more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within centrosome duplication	ISO Inferred from Sequence Orthology more info	PubMed
involved_in chromosome segregation	ISO Inferred from Sequence Orthology more info
involved_in chromosome segregation	ISS Inferred from Sequence or Structural Similarity more info
involved_in chromosome segregation	NAS Non-traceable Author Statement more info	PubMed
involved_in establishment of mitotic spindle orientation	IEA Inferred from Electronic Annotation more info
involved_in establishment of mitotic spindle orientation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within kinetochore organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within metaphase chromosome alignment	ISO Inferred from Sequence Orthology more info	PubMed
involved_in metaphase chromosome alignment	ISO Inferred from Sequence Orthology more info
involved_in metaphase chromosome alignment	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within mitotic cell cycle checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mitotic sister chromatid segregation	ISO Inferred from Sequence Orthology more info
involved_in mitotic spindle assembly checkpoint signaling	NAS Non-traceable Author Statement more info	PubMed
involved_in mitotic spindle organization	ISO Inferred from Sequence Orthology more info
involved_in mitotic spindle organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of mitotic cell cycle spindle assembly checkpoint	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mitotic cell cycle spindle assembly checkpoint	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of protein stability	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within skeletal muscle satellite cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spindle assembly involved in female meiosis I	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within spindle assembly involved in female meiosis I	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Ndc80 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Ndc80 complex	ISO Inferred from Sequence Orthology more info
part_of Ndc80 complex	ISS Inferred from Sequence or Structural Similarity more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in chromosome, centromeric region	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore	ISO Inferred from Sequence Orthology more info
located_in kinetochore	ISS Inferred from Sequence or Structural Similarity more info
located_in kinetochore	NAS Non-traceable Author Statement more info	PubMed
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of outer kinetochore	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: kinetochore protein NDC80 homolog

Names: NDC80 homolog, kinetochore complex component; highly expressed in cancer, rich in leucine heptad repeats; kinetochore associated 2; kinetochore protein Hec1; kinetochore-associated protein 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_023294.2 → NP_075783.2 kinetochore protein NDC80 homolog

See identical proteins and their annotated locations for NP_075783.2

Status: VALIDATED

Source sequence(s)

AF326730, AK011497, BX527376, CJ201671

Consensus CDS

CCDS28959.1

UniProtKB/Swiss-Prot

Q3TQT6, Q3UWM5, Q99P70, Q9D0F1

UniProtKB/TrEMBL

A0A3B2WAS6

Related

ENSMUSP00000024851.10, ENSMUST00000024851.10

Conserved Domains (3) summary

COG5185
Location:48 → 619

HEC1; Protein involved in chromosome segregation, interacts with SMC proteins [Cell cycle control, cell division, chromosome partitioning]

pfam03801
Location:51 → 205

Ndc80_HEC; HEC/Ndc80p family

cl19219
Location:324 → 394

DUF342; Protein of unknown function (DUF342)