Rasgrp4 RAS guanyl releasing protein 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rasgrp4provided by MGI
Official Full Name: RAS guanyl releasing protein 4provided by MGI
Primary source: MGI:MGI:2386851
See related: Ensembl:ENSMUSG00000030589 AllianceGenome:MGI:2386851
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Enables diacylglycerol binding activity. Involved in antibacterial innate immune response; phospholipase C-activating G protein-coupled receptor signaling pathway; and positive regulation of natural killer cell mediated immunity. Located in cytoplasmic side of plasma membrane. Is expressed in several structures, including genitourinary system; liver; peritoneal cavity; spleen; and tongue. Orthologous to human RASGRP4 (RAS guanyl releasing protein 4). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in thymus adult (RPKM 8.9), duodenum adult (RPKM 8.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 16.94 cM; 7 B1

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (28834358..28853386)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (29134607..29153961)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RasGRP4 is required for CD117+ MCs and DCs to optimally induce acute NK cell-dependent immune responses to LPS helps explain why this signaling protein has been conserved in evolution
Title: CD117+ Dendritic and Mast Cells Are Dependent on RasGRP4 to Function as Accessory Cells for Optimal Natural Killer Cell-Mediated Responses to Lipopolysaccharide.
Rasgrp4 is an activity dependent gene in mouse olfactory sensory neurons. [Rasgrp4]
Title: Activity-dependent genes in mouse olfactory sensory neurons.
GPCR activation of Ras and PI3Kc in neutrophils depends on PLCb2/b3 and the RasGEF RasGRP4.
Title: GPCR activation of Ras and PI3Kc in neutrophils depends on PLCb2/b3 and the RasGEF RasGRP4.
Ras guanine nucleotide-releasing protein-4 (RasGRP4) involvement in experimental arthritis and colitis.
Title: Ras guanine nucleotide-releasing protein-4 (RasGRP4) involvement in experimental arthritis and colitis.
Despite high levels of RasGRP4 expression in mast cells, RasGRP4 deficiency led to only a modest reduction in FcepsilonRI-mediated degranulation and cytokine production.
Title: The role of Ras guanine nucleotide releasing protein 4 in Fc epsilonRI-mediated signaling, mast cell function, and T cell development.
RasGRP4 cooperates with AML1 mutations in T cell leukemogenesis as a class I mutation
Title: Possible involvement of RasGRP4 in leukemogenesis.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (7) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI836081 (e-PCR)
- UniSTS:164352

AV026483 (e-PCR)
- UniSTS:179545

Rasgrp4 (e-PCR), detects polymorphism
- UniSTS:508522

NoName (e-PCR)
- UniSTS:235635

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables diacylglycerol binding	IDA Inferred from Direct Assay more info	PubMed
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info
enables guanyl-nucleotide exchange factor activity	ISS Inferred from Sequence or Structural Similarity more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Ras protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in antibacterial innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of natural killer cell mediated immunity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in small GTPase-mediated signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasmic side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: RAS guanyl-releasing protein 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001174155.1 → NP_001167626.1 RAS guanyl-releasing protein 4 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC164564, AK049562, BY749980

Consensus CDS

CCDS85258.1

UniProtKB/TrEMBL

E0CZ06

Related

ENSMUSP00000125137.2, ENSMUST00000159975.8

Conserved Domains (4) summary

smart00147
Location:197 → 432

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

cd00029
Location:541 → 595

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam13499
Location:465 → 494

EF-hand_7; EF-hand domain pair

cl02520
Location:82 → 164

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...
NM_145149.4 → NP_660131.2 RAS guanyl-releasing protein 4 isoform 2

See identical proteins and their annotated locations for NP_660131.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

Source sequence(s)

AC164564, AK049562, BY749980

Consensus CDS

CCDS85257.1

UniProtKB/Swiss-Prot

Q3TNQ1, Q3TRL5, Q3U3I4, Q811P7, Q8BKV7, Q8BTM9, Q91ZC8, Q920A2

UniProtKB/TrEMBL

E0CZ06

Related

ENSMUSP00000032811.6, ENSMUST00000032811.12

Conserved Domains (4) summary

smart00147
Location:197 → 432

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

cd00029
Location:541 → 590

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

pfam13499
Location:465 → 494

EF-hand_7; EF-hand domain pair

cl02520
Location:82 → 164

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...

RNA

NR_045676.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses two alternate splice sites in the coding region, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC164564

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

28834358..28853386

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011250525.2 → XP_011248827.1 RAS guanyl-releasing protein 4 isoform X2

UniProtKB/TrEMBL

E0CZ06

Related

ENSMUSP00000124183.2, ENSMUST00000159351.3

Conserved Domains (3) summary

smart00147
Location:197 → 432

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

cd00029
Location:541 → 595

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl02520
Location:82 → 164

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...
XM_017322159.3 → XP_017177648.1 RAS guanyl-releasing protein 4 isoform X1

UniProtKB/TrEMBL

E0CZ06

Conserved Domains (3) summary

smart00147
Location:201 → 436

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

cd00029
Location:545 → 599

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl02520
Location:86 → 168

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...
XM_017322161.3 → XP_017177650.1 RAS guanyl-releasing protein 4 isoform X4

UniProtKB/TrEMBL

E0CZ06

Conserved Domains (3) summary

smart00147
Location:201 → 436

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

cd00029
Location:545 → 599

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl02520
Location:86 → 168

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...
XM_017322160.3 → XP_017177649.1 RAS guanyl-releasing protein 4 isoform X3

UniProtKB/TrEMBL

E0CZ06

Conserved Domains (3) summary

smart00147
Location:201 → 436

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

cd00029
Location:545 → 594

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl02520
Location:86 → 168

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...
XM_017322162.3 → XP_017177651.1 RAS guanyl-releasing protein 4 isoform X5

UniProtKB/TrEMBL

E0CZ06

Conserved Domains (3) summary

smart00147
Location:201 → 436

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

cd00029
Location:545 → 594

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl02520
Location:86 → 168

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...
XM_017322163.3 → XP_017177652.1 RAS guanyl-releasing protein 4 isoform X6

UniProtKB/TrEMBL

E0CZ06

Conserved Domains (3) summary

smart00147
Location:155 → 390

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

cd00029
Location:499 → 553

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl02520
Location:40 → 122

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...
XM_017322164.3 → XP_017177653.1 RAS guanyl-releasing protein 4 isoform X7

UniProtKB/TrEMBL

E0CZ06

Conserved Domains (3) summary

smart00147
Location:201 → 436

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

cl00040
Location:545 → 582

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl02520
Location:86 → 168

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...
XM_017322165.3 → XP_017177654.1 RAS guanyl-releasing protein 4 isoform X8

UniProtKB/TrEMBL

E0CZ06

Conserved Domains (3) summary

smart00147
Location:201 → 436

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

cd00029
Location:545 → 577

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl02520
Location:86 → 168

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...