Cad carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cadprovided by RGD
Official Full Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotaseprovided by RGD
Primary source: RGD:1588606
See related: EnsemblRapid:ENSRNOG00000026474 AllianceGenome:RGD:1588606
Gene type: protein coding
RefSeq status: INFERRED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables several functions, including aspartate carbamoyltransferase activity; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; and dihydroorotase activity. Involved in several processes, including 'de novo' pyrimidine nucleobase biosynthetic process; UTP biosynthetic process; and alpha-amino acid metabolic process. Located in neuronal cell body and terminal bouton. Part of protein-containing complex. Used to study hepatocellular carcinoma. Biomarker of hepatocellular carcinoma; renal cell carcinoma; and rhabdomyosarcoma. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 50. Orthologous to human CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Adrenal (RPKM 103.7), Thymus (RPKM 91.9) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 6q14

Exon count:: 45

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	6	NC_086024.1 (31012091..31035098, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	6	NC_051341.1 (25292133..25315078, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	6	NC_005105.4 (26657507..26680459, complement)

Chromosome 6 - NC_086024.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We conclude that dihydroorotate dehydrogenase can generate superoxide and/or H2O2 directly at low rates and is also capable of indirect production at higher rates from other sites through its ability to reduce the ubiquinone pool.
Title: Production of superoxide/H2O2 by dihydroorotate dehydrogenase in rat skeletal muscle mitochondria.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MARC4015-4016 (e-PCR)
- UniSTS:207115

MARC_4015-4016:996679280:1 (e-PCR)
- UniSTS:207115

Cad (e-PCR), detects polymorphism
- UniSTS:546687

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables amino acid binding	IEA Inferred from Electronic Annotation more info
enables aspartate carbamoyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables aspartate carbamoyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables aspartate carbamoyltransferase activity	ISO Inferred from Sequence Orthology more info
enables carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	IDA Inferred from Direct Assay more info	PubMed
enables carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	ISO Inferred from Sequence Orthology more info
enables dihydroorotase activity	IBA Inferred from Biological aspect of Ancestor more info
enables dihydroorotase activity	IDA Inferred from Direct Assay more info	PubMed
enables dihydroorotase activity	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in 'de novo' UMP biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in 'de novo' UMP biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in 'de novo' pyrimidine nucleobase biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in 'de novo' pyrimidine nucleobase biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in 'de novo' pyrimidine nucleobase biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in UDP biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in UTP biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in animal organ regeneration	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to epidermal growth factor stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to xenobiotic stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in citrulline biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in female pregnancy	IEP Inferred from Expression Pattern more info	PubMed
involved_in glutamine metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glutamine metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glutamine metabolic process	IEP Inferred from Expression Pattern more info	PubMed
involved_in heart development	IEP Inferred from Expression Pattern more info	PubMed
involved_in lactation	IEP Inferred from Expression Pattern more info	PubMed
involved_in liver development	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to amine	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to caffeine	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to cortisol	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to insulin	IDA Inferred from Direct Assay more info	PubMed
involved_in response to starvation	IDA Inferred from Direct Assay more info	PubMed
involved_in response to testosterone	IEP Inferred from Expression Pattern more info	PubMed
involved_in xenobiotic metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell projection	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear matrix	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
located_in terminal bouton	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: multifunctional protein CAD

Names: carbamyl phosphatate synthetase 2

NP_001099180.2

EC 2.1.3.2

XP_063117612.1

EC 2.1.3.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001105710.2 → NP_001099180.2 multifunctional protein CAD

Status: INFERRED

Source sequence(s)

JAXUCZ010000006

UniProtKB/TrEMBL

D4A8A0

Related

ENSRNOP00000076628.1, ENSRNOT00000101016.2

Conserved Domains (13) summary

cd01744
Location:178 → 354

GATase1_CPSase; Small chain of the glutamine-dependent form of carbamoyl phosphate synthase, CPSase II

PRK09060
Location:1429 → 1810

PRK09060; dihydroorotase; Validated

PRK00856
Location:1920 → 2224

pyrB; aspartate carbamoyltransferase catalytic subunit; Provisional

PRK12564
Location:1 → 355

PRK12564; carbamoyl phosphate synthase small subunit; Reviewed

cd01316
Location:1460 → 1806

CAD_DHOase; The eukaryotic CAD protein is a trifunctional enzyme of carbamoylphosphate synthetase-aspartate transcarbamoylase-dihydroorotase, which catalyzes the first three steps of de novo pyrimidine nucleotide biosynthesis. Dihydroorotase (DHOase) catalyzes the ...

cd01423
Location:1313 → 1438

MGS_CPS_I_III; Methylglyoxal synthase-like domain found in pyr1 and URA1-like carbamoyl phosphate synthetases (CPS), including ammonia-dependent CPS Type I, and glutamine-dependent CPS Type III. These are multidomain proteins, in which MGS is the C-terminal domain.

TIGR01369
Location:391 → 1440

CPSaseII_lrg; carbamoyl-phosphate synthase, large subunit

pfam00185
Location:2072 → 2221

OTCace; Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain

pfam00988
Location:2 → 138

CPSase_sm_chain; Carbamoyl-phosphate synthase small chain, CPSase domain

pfam02729
Location:1925 → 2065

OTCace_N; Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain

pfam02786
Location:514 → 716

CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain

pfam02787
Location:801 → 921

CPSase_L_D3; Carbamoyl-phosphate synthetase large chain, oligomerization domain

cl17255
Location:1048 → 1248

ATP-grasp_4; ATP-grasp domain