Fbxw8 F-box and WD-40 domain protein 8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Fbxw8provided by MGI
Official Full Name: F-box and WD-40 domain protein 8provided by MGI
Primary source: MGI:MGI:1923041
See related: Ensembl:ENSMUSG00000032867 AllianceGenome:MGI:1923041
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: FBW6; FBW8; Fbx29; FBXO29; 4930438M06Rik
Summary: Enables ubiquitin-like ligase-substrate adaptor activity. Involved in positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; and ubiquitin-dependent protein catabolic process. Acts upstream of or within labyrinthine layer blood vessel development and spongiotrophoblast layer development. Part of Cul7-RING ubiquitin ligase complex and SCF ubiquitin ligase complex. Is active in cytosol. Is expressed in several structures, including heart; liver; lung; placenta; and spleen. Orthologous to human FBXW8 (F-box and WD repeat domain containing 8). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in ovary adult (RPKM 39.6), subcutaneous fat pad adult (RPKM 36.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 F; 5 57.92 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (118203046..118293523, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (118064981..118155458, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

FBXW8 and PARK2 are sequestrated into insolubility by ATXN2 PolyQ expansions, but only FBXW8 expression is dysregulated
Title: Both ubiquitin ligases FBXW8 and PARK2 are sequestrated into insolubility by ATXN2 PolyQ expansions, but only FBXW8 expression is dysregulated.
mTORC2 negatively feeds back to IRS-1 via control of Fbw8 stability and localization.
Title: mTOR complex 2 regulates proper turnover of insulin receptor substrate-1 via the ubiquitin ligase subunit Fbw8.
our data support the concept that expanded ATXN2 undergoes progressive insolubility and affects PABPC1 by a toxic gain-of-function mechanism with tissue-specific effects, which may be partially alleviated by the induction of FBXW8.
Title: ATXN2-CAG42 sequesters PABPC1 into insolubility and induces FBXW8 in cerebellum of old ataxic knock-in mice.
Depletion of FBXW8 caused a significant accumulation of cyclin D1, as well as sequestration of CDK1 in the cytoplasm. This resulted in a severe reduction of cell proliferation.
Title: A critical role for FBXW8 and MAPK in cyclin D1 degradation and cancer cell proliferation.
FBXW8-CUL7 complex plays a significant role in growth control
Title: Disruption of the Fbxw8 gene results in pre- and postnatal growth retardation in mice.
Cul7 forms a heterodimeric complex with Cul1 in a manner dependent on Fbxw8.
Title: Fbxw8 is essential for Cul1-Cul7 complex formation and for placental development.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Gene trapped (1) 1 citation
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:235289

NoName (e-PCR)
- UniSTS:235291

BB357976 (e-PCR)
- UniSTS:207859

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase activity	IC Inferred by Curator more info	PubMed
enables ubiquitin-like ligase-substrate adaptor activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
contributes_to ubiquitin-protein transferase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in Golgi organization	ISO Inferred from Sequence Orthology more info
involved_in Golgi organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in cell population proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in cilium assembly	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within labyrinthine layer blood vessel development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of dendrite morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of dendrite morphogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within spongiotrophoblast layer development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	IC Inferred by Curator more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of 3M complex	ISO Inferred from Sequence Orthology more info
part_of 3M complex	ISS Inferred from Sequence or Structural Similarity more info
part_of Cul7-RING ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Cul7-RING ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
part_of Cul7-RING ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in Golgi apparatus	ISS Inferred from Sequence or Structural Similarity more info
part_of SCF ubiquitin ligase complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in centriole	IBA Inferred from Biological aspect of Ancestor more info
is_active_in ciliary basal body	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: F-box/WD repeat-containing protein 8

Names: F-box and WD-40 domain-containing protein 8; F-box protein FBX29

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_172721.2 → NP_766309.2 F-box/WD repeat-containing protein 8

See identical proteins and their annotated locations for NP_766309.2

Status: VALIDATED

Source sequence(s)

AK034447, AK053794, BY468586

Consensus CDS

CCDS19609.1

UniProtKB/Swiss-Prot

Q8BI62, Q8BI75, Q8BI76, Q8BIA4, Q8CID8, Q921Z1

Related

ENSMUSP00000047012.8, ENSMUST00000049474.9

Conserved Domains (4) summary

COG2319
Location:263 → 588

WD40; WD40 repeat [General function prediction only]

pfam12937
Location:118 → 162

F-box-like; F-box-like

sd00039
Location:213 → 252

7WD40; WD40 repeat [structural motif]

cl02567
Location:209 → 501

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...