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    laf-1 ATP-dependent RNA helicase laf-1 [ Caenorhabditis elegans ]

    Gene ID: 190611, updated on 9-Dec-2024

    Summary

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    Official Symbol
    laf-1
    Official Full Name
    ATP-dependent RNA helicase laf-1
    Primary source
    WormBase:WBGene00002244
    Locus tag
    CELE_Y71H2AM.19
    See related
    AllianceGenome:WB:WBGene00002244
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Caenorhabditis elegans (strain: Bristol N2)
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
    Summary
    Enables RNA strand annealing activity; identical protein binding activity; and molecular condensate scaffold activity. Involved in several processes, including masculinization of hermaphroditic germ-line; positive regulation of embryonic development; and positive regulation of fertilization. Located in P granule. Is expressed in germ line. Human ortholog(s) of this gene implicated in colorectal cancer and syndromic X-linked intellectual disability. Orthologous to human DDX3X (DEAD-box helicase 3 X-linked) and DDX3Y (DEAD-box helicase 3 Y-linked). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See laf-1 in Genome Data Viewer
    Location:
    chromosome: III
    Exon count:
    6
    Sequence:
    Chromosome: III; NC_003281.10 (2769356..2779053, complement)

    Chromosome III - NC_003281.10Genomic Context describing neighboring genes Neighboring gene intestinal acid PHOsphatase Neighboring gene ncRNA Neighboring gene HMG box domain-containing protein Neighboring gene ncRNA Neighboring gene DNA/pantothenate metabolism flavoprotein C-terminal domain-containing protein;Myotubularin phosphatase domain-containing protein Neighboring gene Serine/threonine-protein phosphatase 2A activator Neighboring gene Cytochrome oxidase c subunit VIb

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003281.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Phase behaviour of disordered proteins underlying low density and high permeability of liquid organelles. Wei MT, et al. Nat Chem, 2017 Nov. PMID 29064502, Free PMC Article
    2. Controllable protein phase separation and modular recruitment to form responsive membraneless organelles. Schuster BS, et al. Nat Commun, 2018 Jul 30. PMID 30061688, Free PMC Article
    3. Methods and Strategies to Quantify Phase Separation of Disordered Proteins. Ceballos AV, et al. Methods Enzymol, 2018. PMID 30471691, Free PMC Article
    4. Quantifying Dynamics in Phase-Separated Condensates Using Fluorescence Recovery after Photobleaching. Taylor NO, et al. Biophys J, 2019 Oct 1. PMID 31540706, Free PMC Article
    5. Incorporation and Assembly of a Light-Emitting Enzymatic Reaction into Model Protein Condensates. Guan M, et al. Biochemistry, 2021 Oct 26. PMID 34648259, Free PMC Article

    See all (43) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Identifying sequence perturbations to an intrinsically disordered protein that determine its phase-separation behavior.
      Title: Identifying sequence perturbations to an intrinsically disordered protein that determine its phase-separation behavior.
    2. The article describes RNA remodeling activity of human DDX3X and Caenorhabditis elegans LAF-1 tuned by protein concentration, RNA length, and ATP.
      Title: RNA Remodeling Activity of DEAD Box Proteins Tuned by Protein Concentration, RNA Length, and ATP.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by WormBase

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA strand annealing activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables mRNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables molecular condensate scaffold activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables nucleic acid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in cell differentiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in gamete generation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in masculinization of hermaphroditic germ-line IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in masculinization of hermaphroditic germ-line IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of gene expression IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in negative regulation of gene expression IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of embryonic development IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in positive regulation of embryonic development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of fertilization IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in positive regulation of fertilization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in P granule IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in P granule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in P granule IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of canonical inflammasome complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cell leading edge ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    located_in cell projection IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasmic stress granule IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in lamellipodium IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    ATP-dependent RNA helicase laf-1
    NP_001254859.1
    • Confirmed by transcript evidence

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003281.10 Reference assembly

      Range
      2769356..2779053 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001267930.4 → NP_001254859.1  ATP-dependent RNA helicase laf-1 [Caenorhabditis elegans]

      See identical proteins and their annotated locations for NP_001254859.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D0PV95, Q4W5R4
      Conserved Domains (2) summary
      cd18787
      Location:466 → 596
      SF2_C_DEAD; C-terminal helicase domain of the DEAD box helicases
      cd17967
      Location:233 → 460
      DEADc_DDX3_DDX4; DEAD-box helicase domain of ATP-dependent RNA helicases DDX3 and DDX4
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    D0PV95.1

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