Spire2 spire type actin nucleation factor 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Spire2provided by MGI
Official Full Name: spire type actin nucleation factor 2provided by MGI
Primary source: MGI:MGI:2446256
See related: Ensembl:ENSMUSG00000010154 AllianceGenome:MGI:2446256
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Spir2; Spir-2
Summary: Predicted to enable microtubule binding activity. Involved in several processes, including cytoskeleton organization; cytoskeleton-dependent cytokinesis; and intracellular transport. Located in cell cortex and cytoplasmic vesicle membrane. Colocalizes with cleavage furrow. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; heart ventricle; and limb. Orthologous to human SPIRE2 (spire type actin nucleation factor 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in colon adult (RPKM 27.4), stomach adult (RPKM 18.7) and 17 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 E1; 8 72.1 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (124059452..124096257)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (123332713..123369518)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

After injection of double-strand RNA against Spire 1 and 2 (Spire1+2 double-strand RNA), actin mesh levels were significantly decreased compared with that of control (negative). Conserved zinc-binding cysteines in the FYVE domain of Spire2 are crucial for proper localization in the oocyte cortex and cytoplasmic vesicles. The interaction between FMN2 and Spire2 proteins does not affect localization of Spire2.
Title: Spire localization via zinc finger-containing domain is crucial for the asymmetric division of mouse oocyte.
The authors determined crystal structures of MyoVa-globular tail domain bound either to the Spir-2 motif or to Rab11 and show that a Spir-2:MyoVa:Rab11 complex can form.
Title: Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes.
We describe a novel locus, called Streptococcus pneumoniae infection resistance 2 (Spir2) on Chr4, which influences time to morbidity and the development of bacteraemia post-infection.
Title: Spir2; a novel QTL on chromosome 4 contributes to susceptibility to pneumococcal infection in mice.
Spire1 and Spire2 drive two critical steps of asymmetric oocyte division
Title: Spire-type actin nucleators cooperate with Formin-2 to drive asymmetric oocyte division.
Spir-2 is found to be expressed as a single mRNA in the developing nervous system and intestine. In adult mouse it is expressed in epithelial cells of the digestive tract, in neuronal cells of the nervous system, and in testical spermatocytes.
Title: Expression patterns of the mouse Spir-2 actin nucleator.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in Golgi vesicle transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in actin filament network formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament polymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin nucleation	IEA Inferred from Electronic Annotation more info
involved_in cleavage furrow formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cleavage furrow formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of meiotic spindle localization	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of meiotic spindle localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of meiotic spindle localization	ISO Inferred from Sequence Orthology more info
involved_in formin-nucleated actin cable assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in formin-nucleated actin cable assembly	ISO Inferred from Sequence Orthology more info
involved_in formin-nucleated actin cable assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in intracellular transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in polar body extrusion after meiotic divisions	IBA Inferred from Biological aspect of Ancestor more info
involved_in polar body extrusion after meiotic divisions	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of double-strand break repair	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of double-strand break repair	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in vesicle-mediated transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cell cortex	IBA Inferred from Biological aspect of Ancestor more info
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with cleavage furrow	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasmic vesicle membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic vesicle membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein spire homolog 2

Names: spire homolog 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_172287.2 → NP_758491.1 protein spire homolog 2

See identical proteins and their annotated locations for NP_758491.1

Status: PROVISIONAL

Source sequence(s)

BC049152

Consensus CDS

CCDS22754.1

UniProtKB/Swiss-Prot

Q8K1S6, Q8R0R2

Related

ENSMUSP00000010298.7, ENSMUST00000010298.7

Conserved Domains (3) summary

cd15768
Location:570 → 684

FYVE_SPIR2; FYVE-related domain found in protein spire homolog 2 (Spire2) and similar proteins

pfam16474
Location:26 → 212

KIND; Kinase non-catalytic C-lobe domain

cl19549
Location:174 → 351

DUF505; Protein of unknown function (DUF505)