Eps15 epidermal growth factor receptor pathway substrate 15 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Eps15provided by RGD
Official Full Name: epidermal growth factor receptor pathway substrate 15provided by RGD
Primary source: RGD:1305550
See related: EnsemblRapid:ENSRNOG00000010299 AllianceGenome:RGD:1305550
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: RGD1307641
Summary: Predicted to enable identical protein binding activity; polyubiquitin modification-dependent protein binding activity; and ubiquitin binding activity. Involved in several processes, including endocytic recycling; positive regulation of receptor recycling; and postsynaptic neurotransmitter receptor internalization. Located in apical plasma membrane; basal plasma membrane; and early endosome. Is active in glutamatergic synapse and postsynaptic endocytic zone. Orthologous to human EPS15 (epidermal growth factor receptor pathway substrate 15). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Spleen (RPKM 300.5), Brain (RPKM 275.3) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 5q34-q35

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	5	NC_086023.1 (129274487..129374856)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	5	NC_051340.1 (124045865..124146221)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	5	NC_005104.4 (128923809..129022268)

Chromosome 5 - NC_086023.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Knockdown of Eps15 suppresses the internalization of GluA1 but not the mutant GluA1 that lacks ubiquitination sites, indicating a role of Eps15 for the internalization of ubiquitinated AMPARs
Title: Endocytic adaptor epidermal growth factor receptor substrate 15 (Eps15) is involved in the trafficking of ubiquitinated α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors.
distinct forms of Eps15 direct EGFR to either the late endosome/lysosome for degradation (Eps15) or to the recycling endosome for transit back to the cell surface (Eps15S)
Title: Recycling of the epidermal growth factor receptor is mediated by a novel form of the clathrin adaptor protein Eps15.
FCHO2 regulates clathrin-mediated endocytosis through its interactions with membranes and Eps15.
Title: Characterization of the EFC/F-BAR domain protein, FCHO2.
Nedd4-mediated ubiquitination of Cx43 is required to recruit Eps15, through its ubiquitin-interacting motif, and targets ubiquitinated Cx43 to the endocytic pathway.
Title: Eps15 interacts with ubiquitinated Cx43 and mediates its internalization.
Eps15 plays an important role in clathrin-coated vesicle formation not only at the plasma membrane but also at the TGN during the secretory process
Title: Eps15 mediates vesicle trafficking from the trans-Golgi network via an interaction with the clathrin adaptor AP-1.
Binds Stonin2 at the second EH domain.
Title: Structure of the Eps15-stonin2 complex provides a molecular explanation for EH-domain ligand specificity.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D5Got134 (e-PCR)
- UniSTS:112164

D5Rat95 (e-PCR)
- UniSTS:118940

D5Got46 (e-PCR)
- UniSTS:113870

RH131227 (e-PCR)
- UniSTS:214510

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables polyubiquitin modification-dependent protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in Golgi to endosome transport	ISO Inferred from Sequence Orthology more info
involved_in clathrin coat assembly	ISO Inferred from Sequence Orthology more info
involved_in endocytic recycling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in endosomal transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of receptor recycling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in postsynaptic neurotransmitter receptor internalization	IDA Inferred from Direct Assay more info	PubMed
involved_in postsynaptic neurotransmitter receptor internalization	IEP Inferred from Expression Pattern more info	PubMed
involved_in postsynaptic neurotransmitter receptor internalization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in receptor-mediated endocytosis of virus by host cell	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of protein localization	ISO Inferred from Sequence Orthology more info
involved_in symbiont entry into host cell	ISO Inferred from Sequence Orthology more info
involved_in ubiquitin-dependent endocytosis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of AP-2 adaptor complex	ISO Inferred from Sequence Orthology more info
located_in aggresome	ISO Inferred from Sequence Orthology more info
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in basal plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in ciliary membrane	ISO Inferred from Sequence Orthology more info
part_of clathrin coat of coated pit	IBA Inferred from Biological aspect of Ancestor more info
part_of clathrin coat of coated pit	ISO Inferred from Sequence Orthology more info
located_in clathrin-coated pit	ISO Inferred from Sequence Orthology more info
located_in clathrin-coated vesicle	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in early endosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic endocytic zone	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic endocytic zone	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: epidermal growth factor receptor substrate 15

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001365390.1 → NP_001352319.1 epidermal growth factor receptor substrate 15

Status: VALIDATED

Source sequence(s)

AB262963, JAXUCZ010000005

UniProtKB/TrEMBL

A0A8I5YC99, A7BFV9

Related

ENSRNOP00000084473.1, ENSRNOT00000116370.2

Conserved Domains (2) summary

smart00027
Location:121 → 215

EH; Eps15 homology domain

cl25732
Location:333 → 504

SMC_N; RecF/RecN/SMC N terminal domain

RNA

NR_158458.2 RNA Sequence

Status: VALIDATED

Source sequence(s)

JAXUCZ010000005

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086023.1 Reference GRCr8

Range

129274487..129374856

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063287709.1 → XP_063143779.1 epidermal growth factor receptor substrate 15 isoform X7
XM_039109961.2 → XP_038965889.1 epidermal growth factor receptor substrate 15 isoform X1

UniProtKB/TrEMBL

A0A8I5YC99

Related

ENSRNOP00000077237.2, ENSRNOT00000116078.2

Conserved Domains (2) summary

smart00027
Location:121 → 215

EH; Eps15 homology domain

COG1196
Location:369 → 540

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_039109962.2 → XP_038965890.1 epidermal growth factor receptor substrate 15 isoform X6

UniProtKB/TrEMBL

Q5JC29

Conserved Domains (2) summary

smart00027
Location:121 → 215

EH; Eps15 homology domain

COG1196
Location:369 → 540

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_063287710.1 → XP_063143780.1 epidermal growth factor receptor substrate 15 isoform X8
XM_063287708.1 → XP_063143778.1 epidermal growth factor receptor substrate 15 isoform X5

Related

ENSRNOP00000059209.4, ENSRNOT00000064145.4
XM_063287706.1 → XP_063143776.1 epidermal growth factor receptor substrate 15 isoform X3
XM_063287705.1 → XP_063143775.1 epidermal growth factor receptor substrate 15 isoform X2
XM_063287707.1 → XP_063143777.1 epidermal growth factor receptor substrate 15 isoform X4

Related

ENSRNOP00000089899.2, ENSRNOT00000098058.2