Ubr5 ubiquitin protein ligase E3 component n-recognin 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ubr5provided by MGI
Official Full Name: ubiquitin protein ligase E3 component n-recognin 5provided by MGI
Primary source: MGI:MGI:1918040
See related: Ensembl:ENSMUSG00000037487 AllianceGenome:MGI:1918040
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Edd; Edd1
Summary: Enables ubiquitin protein ligase activity. Involved in negative regulation of smoothened signaling pathway and ubiquitin-dependent protein catabolic process via the N-end rule pathway. Acts upstream of or within negative regulation of interleukin-17 production and regulation of protein stability. Predicted to be located in several cellular components, including chromatin; nucleoplasm; and perinuclear region of cytoplasm. Predicted to be part of protein-containing complex. Predicted to be active in cytoplasm and nucleus. Is expressed in cranial ganglion; nervous system; sensory organ; submandibular gland primordium; and vibrissa. Human ortholog(s) of this gene implicated in breast cancer (multiple) and carcinoma (multiple). Orthologous to human UBR5 (ubiquitin protein ligase E3 component n-recognin 5). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in testis adult (RPKM 22.8), CNS E11.5 (RPKM 15.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 B3.1; 15 15.1 cM

Exon count:: 60

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (37966421..38084103, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (37967328..38078862, complement)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

UBR5 promotes antiviral immunity by disengaging the transcriptional brake on RIG-I like receptors.
Title: UBR5 promotes antiviral immunity by disengaging the transcriptional brake on RIG-I like receptors.
Ubiquitin-protein ligase Ubr5 cooperates with hedgehog signalling to promote skeletal tissue homeostasis.
Title: Ubiquitin-protein ligase Ubr5 cooperates with hedgehog signalling to promote skeletal tissue homeostasis.
UBR5 HECT domain mutations identified in mantle cell lymphoma control maturation of B cells.
Title: UBR5 HECT domain mutations identified in mantle cell lymphoma control maturation of B cells.
Knockdown of the E3 ubiquitin ligase UBR5 and its role in skeletal muscle anabolism.
Title: Knockdown of the E3 ubiquitin ligase UBR5 and its role in skeletal muscle anabolism.
Tumor derived UBR5 promotes ovarian cancer growth and metastasis through inducing immunosuppressive macrophages.
Title: Tumor derived UBR5 promotes ovarian cancer growth and metastasis through inducing immunosuppressive macrophages.
Data suggest that miRNA-1224-mediated and Ago2-dependent modulation of spinal circRNA-Filip1l expression regulates nociception via targeting Ubr5, revealing a novel epigenetic mechanism of interaction between miRNA and circRNA in chronic inflammation pain.
Title: MicroRNA-1224 Splicing CircularRNA-Filip1l in an Ago2-Dependent Manner Regulates Chronic Inflammatory Pain via Targeting Ubr5.
Hence, while loss of UBR5 perturbed Hedgehog signalling in the developing limb, there were no obvious morphological defects.
Title: Use of a Conditional Ubr5 Mutant Allele to Investigate the Role of an N-End Rule Ubiquitin-Protein Ligase in Hedgehog Signalling and Embryonic Limb Development.
Findings unveil UBR5 as a novel and critical regulator of tumor growth, metastasis, and immune response in triple negative breast cancer.
Title: E3 Ubiquitin Ligase UBR5 Drives the Growth and Metastasis of Triple-Negative Breast Cancer.
UBR5-mediated ATMIN ubiquitination is a vital event for ATM pathway selection and activation in response to DNA damage
Title: UBR5-mediated ubiquitination of ATMIN is required for ionizing radiation-induced ATM signaling and function.
a novel function of RIP1 kinase involving its interaction with EDD to regulate JNK activation and TNFalpha production.
Title: A novel role for RIP1 kinase in mediating TNFα production.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (5) 1 citation
Gene trapped (3) 1 citation
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AW549941 (e-PCR)
- UniSTS:180748

RH126145 (e-PCR)
- UniSTS:162857

RH15795 (e-PCR)
- UniSTS:69326

NoName (e-PCR)
- UniSTS:237286

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin binding	ISS Inferred from Sequence or Structural Similarity more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	ISS Inferred from Sequence or Structural Similarity more info
enables ubiquitin-ubiquitin ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin-ubiquitin ligase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA damage response	ISS Inferred from Sequence or Structural Similarity more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA repair-dependent chromatin remodeling	ISO Inferred from Sequence Orthology more info
involved_in DNA repair-dependent chromatin remodeling	ISS Inferred from Sequence or Structural Similarity more info
involved_in cytoplasm protein quality control	ISO Inferred from Sequence Orthology more info
involved_in cytoplasm protein quality control by the ubiquitin-proteasome system	ISO Inferred from Sequence Orthology more info
involved_in cytoplasm protein quality control by the ubiquitin-proteasome system	ISS Inferred from Sequence or Structural Similarity more info
involved_in heterochromatin boundary formation	ISO Inferred from Sequence Orthology more info
involved_in heterochromatin boundary formation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within negative regulation of interleukin-17 production	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of smoothened signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear protein quality control by the ubiquitin-proteasome system	ISO Inferred from Sequence Orthology more info
involved_in nuclear protein quality control by the ubiquitin-proteasome system	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of canonical Wnt signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of canonical Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of gene expression	ISO Inferred from Sequence Orthology more info	PubMed
involved_in positive regulation of protein import into nucleus	ISO Inferred from Sequence Orthology more info
involved_in progesterone receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in progesterone receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein K11-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein K29-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein K48-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein branched polyubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein polyubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein polyubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of protein stability	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process via the N-end rule pathway	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in chromatin	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info	PubMed

General protein information

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Preferred Names: E3 ubiquitin-protein ligase UBR5

Names: E3 ubiquitin-protein ligase, HECT domain-containing 1; HECT-type E3 ubiquitin transferase UBR5; extraembryonic development; hyperplastic discs protein homolog

NP_001074828.2

EC 2.3.2.26

NP_001106192.1

EC 2.3.2.26

XP_006520242.1

EC 2.3.2.26

XP_006520243.1

EC 2.3.2.26

XP_006520244.1

EC 2.3.2.26

XP_006520245.1

EC 2.3.2.26

XP_030104623.1

EC 2.3.2.26

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001081359.3 → NP_001074828.2 E3 ubiquitin-protein ligase UBR5 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AK014485, AK162029, AY550908, CB527808, CJ063125, DV047629

Consensus CDS

CCDS49595.1

UniProtKB/Swiss-Prot

Q698K9, Q6PEQ8, Q6PFQ9, Q80TP3, Q80VL4, Q810V6, Q9CXE9

UniProtKB/TrEMBL

A0A2I3BQS6

Related

ENSMUSP00000154293.2, ENSMUST00000226414.2

Conserved Domains (5) summary

smart00396
Location:1177 → 1244

ZnF_UBR1; Putative zinc finger in N-recognin, a recognition component of the N-end rule pathway

smart00517
Location:2389 → 2452

PolyA; C-terminal domain of Poly(A)-binding protein. Present also in Drosophila hyperplastics discs protein

smart00119
Location:2467 → 2795

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with

pfam00632
Location:2500 → 2798

HECT; HECT-domain (ubiquitin-transferase)

pfam11547
Location:179 → 226

E3_UbLigase_EDD; E3 ubiquitin ligase EDD
NM_001112721.2 → NP_001106192.1 E3 ubiquitin-protein ligase UBR5 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

Source sequence(s)

AK014485, AK162029, AY550908, CB527808, CJ063125

Consensus CDS

CCDS88751.1

UniProtKB/Swiss-Prot

Q698K9, Q6PEQ8, Q6PFQ9, Q80TP3, Q80VL4, Q810V6, Q9CXE9

UniProtKB/TrEMBL

E9Q2H1

Related

ENSMUSP00000105965.4, ENSMUST00000110336.4

Conserved Domains (5) summary

smart00396
Location:1171 → 1238

ZnF_UBR1; Putative zinc finger in N-recognin, a recognition component of the N-end rule pathway

smart00517
Location:2383 → 2446

PolyA; C-terminal domain of Poly(A)-binding protein. Present also in Drosophila hyperplastics discs protein

smart00119
Location:2461 → 2789

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with

pfam00632
Location:2494 → 2792

HECT; HECT-domain (ubiquitin-transferase)

pfam11547
Location:179 → 226

E3_UbLigase_EDD; E3 ubiquitin ligase EDD

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

37966421..38084103 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006520182.5 → XP_006520245.1 E3 ubiquitin-protein ligase UBR5 isoform X4

UniProtKB/Swiss-Prot

Q698K9, Q6PEQ8, Q6PFQ9, Q80TP3, Q80VL4, Q810V6, Q9CXE9

Conserved Domains (4) summary

smart00396
Location:1171 → 1238

ZnF_UBR1; Putative zinc finger in N-recognin, a recognition component of the N-end rule pathway

smart00517
Location:2385 → 2448

PolyA; C-terminal domain of Poly(A)-binding protein. Present also in Drosophila hyperplastics discs protein

smart00119
Location:2463 → 2791

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with

cd14423
Location:184 → 230

CUE_UBR5; CUE domain found in E3 ubiquitin-protein ligase UBR5 and similar proteins
XM_006520181.5 → XP_006520244.1 E3 ubiquitin-protein ligase UBR5 isoform X3

UniProtKB/Swiss-Prot

Q698K9, Q6PEQ8, Q6PFQ9, Q80TP3, Q80VL4, Q810V6, Q9CXE9

Conserved Domains (4) summary

smart00396
Location:1177 → 1244

ZnF_UBR1; Putative zinc finger in N-recognin, a recognition component of the N-end rule pathway

smart00517
Location:2389 → 2452

PolyA; C-terminal domain of Poly(A)-binding protein. Present also in Drosophila hyperplastics discs protein

smart00119
Location:2498 → 2793

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with

cd14423
Location:184 → 230

CUE_UBR5; CUE domain found in E3 ubiquitin-protein ligase UBR5 and similar proteins
XM_006520180.5 → XP_006520243.1 E3 ubiquitin-protein ligase UBR5 isoform X2

UniProtKB/Swiss-Prot

Q698K9, Q6PEQ8, Q6PFQ9, Q80TP3, Q80VL4, Q810V6, Q9CXE9

Conserved Domains (4) summary

smart00396
Location:1177 → 1244

ZnF_UBR1; Putative zinc finger in N-recognin, a recognition component of the N-end rule pathway

smart00517
Location:2391 → 2454

PolyA; C-terminal domain of Poly(A)-binding protein. Present also in Drosophila hyperplastics discs protein

smart00119
Location:2500 → 2795

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with

cd14423
Location:184 → 230

CUE_UBR5; CUE domain found in E3 ubiquitin-protein ligase UBR5 and similar proteins
XM_006520179.5 → XP_006520242.1 E3 ubiquitin-protein ligase UBR5 isoform X1

UniProtKB/Swiss-Prot

Q698K9, Q6PEQ8, Q6PFQ9, Q80TP3, Q80VL4, Q810V6, Q9CXE9

Conserved Domains (4) summary

smart00396
Location:1177 → 1244

ZnF_UBR1; Putative zinc finger in N-recognin, a recognition component of the N-end rule pathway

smart00517
Location:2391 → 2454

PolyA; C-terminal domain of Poly(A)-binding protein. Present also in Drosophila hyperplastics discs protein

smart00119
Location:2469 → 2797

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with

cd14423
Location:184 → 230

CUE_UBR5; CUE domain found in E3 ubiquitin-protein ligase UBR5 and similar proteins
XM_030248763.2 → XP_030104623.1 E3 ubiquitin-protein ligase UBR5 isoform X5

Conserved Domains (3) summary

smart00396
Location:187 → 254

ZnF_UBR1; Putative zinc finger in N-recognin, a recognition component of the N-end rule pathway

smart00517
Location:1401 → 1464

PolyA; C-terminal domain of Poly(A)-binding protein. Present also in Drosophila hyperplastics discs protein

smart00119
Location:1479 → 1807

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with