Gcnt1 glucosaminyl (N-acetyl) transferase 1, core 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Gcnt1provided by MGI
Official Full Name: glucosaminyl (N-acetyl) transferase 1, core 2provided by MGI
Primary source: MGI:MGI:95676
See related: Ensembl:ENSMUSG00000038843 AllianceGenome:MGI:95676
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: IGnT; C2GNT; C2GnT-L; C2GlcNAcT; B130048E03; C2 GlcNAcT; 5630400D21Rik
Summary: Enables beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity. Involved in O-glycan processing, core 2 and positive regulation of leukocyte tethering or rolling. Acts upstream of or within kidney morphogenesis and tissue morphogenesis. Predicted to be located in Golgi cisterna; extracellular space; and trans-Golgi network. Is expressed in several structures, including alimentary system; genitourinary system; nervous system; sensory organ; and skeleton. Orthologous to human GCNT1 (glucosaminyl (N-acetyl) transferase 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in kidney adult (RPKM 36.0), liver E14 (RPKM 9.3) and 9 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19 B; 19 12.75 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (17303505..17350208, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (17326141..17372844, complement)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Deficiency in the glycosyltransferase Gcnt1 increases susceptibility to tuberculosis through a mechanism involving neutrophils.
Title: Deficiency in the glycosyltransferase Gcnt1 increases susceptibility to tuberculosis through a mechanism involving neutrophils.
we show that Gcnt1 transcription and subsequent P-lselectin ligand induction in Th1 cells is governed by binding of STAT4 and T-bet to a distal enhancer and further regulated by epigenetic marks such as H3K27me3.
Title: Core 2 ß1,6-N-acetylglucosaminyltransferase-I, crucial for P-selectin ligand expression is controlled by a distal enhancer regulated by STAT4 and T-bet in CD4+ T helper cells 1.
simultaneous deletion of the Core2 beta1,6-N-acetylglucosaminyltransferase (C2GnT) enzymes C2GnT1 and C2Gnt2 resulted in delayed expulsion of worms.
Title: Requirement for core 2 O-glycans for optimal resistance to helminth infection.
striking differences between the requirement of FucT-VII and C2GlcNAcT-I for Ligands for E-selectin and P-selectin expression in CD4+ T cells.
Title: Differential regulation and impact of fucosyltransferase VII and core 2 β1,6-N-acetyl-glycosaminyltransferase for generation of E-selectin and P-selectin ligands in murine CD4+ T cells.
Core2 O-glycan structure is essential for expression of SI and DDP-IV during intestinal cell differentiation.
Title: Core2 O-glycan structure is essential for the cell surface expression of sucrase isomaltase and dipeptidyl peptidase-IV during intestinal cell differentiation.
Core2 1-6-N-glucosaminyltransferase-I deficiency protects injured arteries from neointima formation in ApoE-deficient mice
Title: Core2 1-6-N-glucosaminyltransferase-I deficiency protects injured arteries from neointima formation in ApoE-deficient mice.
Core2 1-6-N-glucosaminyltransferase-I is crucial for the formation of atherosclerotic lesions in apolipoprotein E-deficient mice.
Title: Core2 1-6-N-glucosaminyltransferase-I is crucial for the formation of atherosclerotic lesions in apolipoprotein E-deficient mice.
Pancreas carcinoma antigen, C2GnT, fused to invariant chain elicits T-cell response and tumor growth inhibition.
Title: Pancreas carcinoma antigen fused to invariant chain elicits T-cell response and tumor growth inhibition.
These results suggest that the core 2 GlcNAc extended glycan chains on megalin can change the ligand-binding affinity and capacity.
Title: Core 2 GlcNAc modification and megalin ligand-binding activity.
plays a critical role in lymphocyte trafficking during chronic inflammation
Title: Core 2 branching beta1,6-N-acetylglucosaminyltransferase and high endothelial venule-restricted sulfotransferase collaboratively control lymphocyte homing.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (1)
Targeted (2) 1 citation

General gene information

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Markers

D87332 (e-PCR)
- UniSTS:186146

NoName (e-PCR)
- UniSTS:237902

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cell adhesion molecule production	ISO Inferred from Sequence Orthology more info
involved_in cell adhesion molecule production	ISS Inferred from Sequence or Structural Similarity more info
involved_in core 2 O-glycan biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in core 2 O-glycan biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in core 2 O-glycan biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in glycoprotein biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in glycoprotein biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within kidney morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in leukocyte tethering or rolling	ISO Inferred from Sequence Orthology more info
involved_in leukocyte tethering or rolling	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of leukocyte tethering or rolling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to insulin	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within tissue morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi cisterna	ISO Inferred from Sequence Orthology more info
located_in Golgi cisterna	ISS Inferred from Sequence or Structural Similarity more info
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
located_in extracellular space	IEA Inferred from Electronic Annotation more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in trans-Golgi network	IEA Inferred from Electronic Annotation more info
located_in trans-Golgi network	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Names: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (Core 2 branching enzyme) (Core2-GlcNAc-transferase) (C2GNT); core 2 beta-1,6-N-acetylglucosaminyltransferase; core 2-branching enzyme; core2-GlcNAc-transferase; leukocyte type core 2 beta-1,6-N-acetylglucosaminyltransferase

NP_001129956.3

EC 2.4.1.102

NP_034395.4

EC 2.4.1.102

NP_775618.3

EC 2.4.1.102

XP_006526742.1

EC 2.4.1.102

XP_011245446.1

EC 2.4.1.102

XP_017173551.1

EC 2.4.1.102

XP_036017326.1

EC 2.4.1.102

XP_036017327.1

EC 2.4.1.102

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001136484.3 → NP_001129956.3 beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

AC147369, BY431975, BY768580

Consensus CDS

CCDS29687.1

UniProtKB/Swiss-Prot

O35981, Q09324, Q8BRB2

UniProtKB/TrEMBL

Q5D0D8

Related

ENSMUSP00000127835.2, ENSMUST00000169897.2

Conserved Domains (1) summary

pfam02485
Location:123 → 391

Branch; Core-2/I-Branching enzyme
NM_010265.5 → NP_034395.4 beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

AC147369, BY431975, CJ092264

Consensus CDS

CCDS29687.1

UniProtKB/Swiss-Prot

O35981, Q09324, Q8BRB2

UniProtKB/TrEMBL

Q5D0D8

Related

ENSMUSP00000157681.2, ENSMUST00000236139.2

Conserved Domains (1) summary

pfam02485
Location:123 → 391

Branch; Core-2/I-Branching enzyme
NM_173442.5 → NP_775618.3 beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

AC147369, AK045216, BY431975

Consensus CDS

CCDS29687.1

UniProtKB/Swiss-Prot

O35981, Q09324, Q8BRB2

UniProtKB/TrEMBL

Q5D0D8

Related

ENSMUSP00000133935.3, ENSMUST00000174236.8

Conserved Domains (1) summary

pfam02485
Location:123 → 391

Branch; Core-2/I-Branching enzyme

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000085.7 Reference GRCm39 C57BL/6J

Range

17303505..17350208 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011247144.4 → XP_011245446.1 beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase isoform X1

UniProtKB/TrEMBL

Q5D0D8

Conserved Domains (1) summary

pfam02485
Location:127 → 395

Branch; Core-2/I-Branching enzyme
XM_006526679.4 → XP_006526742.1 beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase isoform X1

UniProtKB/TrEMBL

Q5D0D8

Conserved Domains (1) summary

pfam02485
Location:127 → 395

Branch; Core-2/I-Branching enzyme
XM_017318062.3 → XP_017173551.1 beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase isoform X1

UniProtKB/TrEMBL

Q5D0D8

Conserved Domains (1) summary

pfam02485
Location:127 → 395

Branch; Core-2/I-Branching enzyme
XM_036161433.1 → XP_036017326.1 beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase isoform X1

UniProtKB/TrEMBL

Q5D0D8

Conserved Domains (1) summary

pfam02485
Location:127 → 395

Branch; Core-2/I-Branching enzyme
XM_036161434.1 → XP_036017327.1 beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase isoform X1

UniProtKB/TrEMBL

Q5D0D8

Related

ENSMUSP00000157544.2, ENSMUST00000235184.2

Conserved Domains (1) summary

pfam02485
Location:127 → 395

Branch; Core-2/I-Branching enzyme