Duox2 dual oxidase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Duox2provided by MGI
Official Full Name: dual oxidase 2provided by MGI
Primary source: MGI:MGI:3036280
See related: Ensembl:ENSMUSG00000068452 AllianceGenome:MGI:3036280
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: LNOX2; THOX2; NOXEF2; P138-TOX; A430065P05Rik
Summary: Predicted to enable NAD(P)H oxidase H2O2-forming activity; calcium ion binding activity; and superoxide-generating NAD(P)H oxidase activity. Acts upstream of or within several processes, including bone mineralization; endocrine system development; and thyroid hormone metabolic process. Predicted to be located in several cellular components, including cell leading edge; cell surface; and endoplasmic reticulum. Predicted to be part of NADPH oxidase complex. Predicted to be active in plasma membrane. Is expressed in placenta and thyroid gland. Used to study congenital hypothyroidism. Human ortholog(s) of this gene implicated in congenital hypothyroidism and thyroid dyshormonogenesis 6. Orthologous to human DUOX2 (dual oxidase 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in large intestine adult (RPKM 68.1), small intestine adult (RPKM 25.6) and 2 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 E5; 2 60.61 cM

Exon count:: 34

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (122109727..122129221, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (122279246..122298740, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Intestinal Epithelial Inactivity of Dual Oxidase 2 Results in Microbiome-Mediated Metabolic Syndrome.
Title: Intestinal Epithelial Inactivity of Dual Oxidase 2 Results in Microbiome-Mediated Metabolic Syndrome.
Telocytes reduce oxidative stress by downregulating DUOX2 expression in inflamed lungs of mice.
Title: Telocytes reduce oxidative stress by downregulating DUOX2 expression in inflamed lungs of mice.
DUOX2 variants associate with preclinical disturbances in microbiota-immune homeostasis and increased inflammatory bowel disease risk.
Title: DUOX2 variants associate with preclinical disturbances in microbiota-immune homeostasis and increased inflammatory bowel disease risk.
Epithelial TLR4 Signaling Activates DUOX2 to Induce Microbiota-Driven Tumorigenesis.
Title: Epithelial TLR4 Signaling Activates DUOX2 to Induce Microbiota-Driven Tumorigenesis.
All these results reveal a critical role of Duox2 in the infection-induced H2O2 production and the H2O2-mediated immune response to infection in the respiratory tract.
Title: Chemiluminescence imaging of Duox2-derived hydrogen peroxide for longitudinal visualization of biological response to viral infection in nasal mucosa.
The findings illustrate for the first time that the extreme C-terminus of Duox2 is required for the functional activity of the enzyme .
Title: CRISPR/Cas9-mediated modification of the extreme C-terminus impairs PDGF-stimulated activity of Duox2.
Duox2 is necessary for the regulation of IFN secretion in in vivo lung, and pulmonary administration of Duox2 DNA using cationic polymer triggers the induction of type I and III IFNs resulting in more complete suppression of influenza A virus infection.
Title: Intranasal delivery of Duox2 DNA using cationic polymer can prevent acute influenza A viral infection in vivo lung.
Both Nox1 and Duox2 induce exfoliation of crypt epithelium, but only Nox1 induces apoptosis. NOX1 and DUOX2 may be potential therapeutic targets for treating ileocolitis in human patients suffering inflammatory bowel disease (IBD).
Title: Deficiency in Duox2 activity alleviates ileitis in GPx1- and GPx2-knockout mice without affecting apoptosis incidence in the crypt epithelium.
dual oxidase-2 and IL-6 play important roles in GPR43-mediated skin inflammation.
Title: GPR43 activation enhances psoriasis-like inflammation through epidermal upregulation of IL-6 and dual oxidase 2 signaling in a murine model.
We propose that Duox2 is responsible for IFN-independent signaling for induction of pattern recognition receptors transcription and can control acute Influenza virus A infection at the beginning of infection.
Title: Duox2 is required for the transcription of pattern recognition receptors in acute viral lung infection: An interferon-independent regulatory mechanism.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (5) 1 citation
Spontaneous (1) 1 citation
Targeted (2) 1 citation

General gene information

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Markers

Duox1 (e-PCR), detects polymorphism
- UniSTS:462682

Duox2 (e-PCR), detects polymorphism
- UniSTS:546746

Duox2 (e-PCR), detects polymorphism
- UniSTS:525522

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables NAD(P)H oxidase H2O2-forming activity	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables superoxide-generating NAD(P)H oxidase activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within adenohypophysis morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within bone mineralization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cytokine-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in defense response	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within hormone metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hydrogen peroxide biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within inner ear development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within multicellular organism growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell motility	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of wound healing	ISO Inferred from Sequence Orthology more info
involved_in response to cAMP	ISO Inferred from Sequence Orthology more info
involved_in response to virus	ISO Inferred from Sequence Orthology more info
involved_in superoxide anion generation	IBA Inferred from Biological aspect of Ancestor more info
involved_in superoxide anion generation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within thyroid gland development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within thyroid hormone metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of NADPH oxidase complex	IBA Inferred from Biological aspect of Ancestor more info
located_in apical part of cell	ISO Inferred from Sequence Orthology more info
located_in cell leading edge	ISO Inferred from Sequence Orthology more info
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: dual oxidase 2

NP_001349684.1

EC 1.6.3.1

XP_006499173.1

EC 1.6.3.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001362755.1 → NP_001349684.1 dual oxidase 2 precursor

Status: VALIDATED

Source sequence(s)

AL844566

Consensus CDS

CCDS89548.1

UniProtKB/TrEMBL

A0A494BAW1, B7ZWM4

Related

ENSMUSP00000158204.2, ENSMUST00000237546.2

Conserved Domains (4) summary

cd06186
Location:1271 → 1545

NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...

cd09820
Location:38 → 594

dual_peroxidase_like; Dual oxidase and related animal heme peroxidases

cd00051
Location:824 → 885

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam01794
Location:1085 → 1229

Ferric_reduct; Ferric reductase like transmembrane component

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

122109727..122129221 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006499110.3 → XP_006499173.1 dual oxidase 2 isoform X1

UniProtKB/TrEMBL

A0A494BAW1, B7ZWM4

Conserved Domains (4) summary

cd06186
Location:1271 → 1545

NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...

cd09820
Location:38 → 594

dual_peroxidase_like; Dual oxidase and related animal heme peroxidases

cd00051
Location:824 → 885

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam01794
Location:1085 → 1229

Ferric_reduct; Ferric reductase like transmembrane component

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_177610.2: Suppressed sequence

Description

NM_177610.2: This RefSeq was removed because currently there is insufficient support for the transcript.