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    SPAM1 sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) [ Bos taurus (domestic cattle) ]

    Gene ID: 353352, updated on 29-Jan-2025

    Summary

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    Gene symbol
    SPAM1
    Gene description
    sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
    See related
    BGD:BT23575; Ensembl:ENSBTAG00000004640
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Bos taurus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos
    Also known as
    PH-20
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    Genomic context

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    See SPAM1 in Genome Data Viewer
    Location:
    chromosome: 4
    Exon count:
    4
    Annotation release Status Assembly Chr Location
    RS_2024_12 current ARS-UCD2.0 (GCF_002263795.3) 4 NC_037331.1 (88202791..88222320)

    Chromosome 4 - NC_037331.1Genomic Context describing neighboring genes Neighboring gene small nucleolar RNA U3 Neighboring gene hyaluronidase 4 Neighboring gene uncharacterized LOC132345126 Neighboring gene uncharacterized LOC112446513

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_037331.1 Chromosome 4 Reference ARS-UCD2.0 Primary Assembly

    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Dominette sequence data
    • BioProject: PRJNA294306
    • Analysis date: Tue Dec 17 08:57:05 2024

    Bibliography

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    Related articles in PubMed

    1. SPAM1 and PH-20 are two gene products expressed in bovine testis and present in sperm. Saindon AA, et al. Reproduction, 2018 Dec. PMID 30328344
    2. Stratification of chondroitin sulfate binding sites in 3D-model of bovine testicular hyaluronidase and effective size of glycosaminoglycan coat of the modified protein. Maksimenko AV, et al. Biochemistry (Mosc), 2015 Mar. PMID 25761683
    3. Effects of divalent cations on bovine testicular hyaluronidase catalyzed transglycosylation of chondroitin sulfates. Kakizaki I, et al. Biochem Biophys Res Commun, 2011 Mar 11. PMID 21320470
    4. SPAM1 isoforms from two tissue origins are differentially localized within ejaculated bull sperm membranes and have different roles during fertilization. Morin G, et al. Biol Reprod, 2010 Feb. PMID 19812301
    5. Development of a fluorescent substrate to measure hyaluronidase activity. Zhang LS, et al. Anal Biochem, 2008 Aug 1. PMID 18492482, Free PMC Article

    See all (9) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. PH-20 transcripts are found in bovine testis and the protein is present in ejaculated spermatozoa.
      Title: SPAM1 and PH-20 are two gene products expressed in bovine testis and present in sperm.
    2. Bovine testicular hyaluronidase 3D-model demonstrated lysine residue stratification during enzyme modification.
      Title: Stratification of chondroitin sulfate binding sites in 3D-model of bovine testicular hyaluronidase and effective size of glycosaminoglycan coat of the modified protein.
    3. the effect of metal cations on the transglycosylation by bovine testicular hyaluronidase using as substrates metal salts of glycosaminoglycan chains was investigated.
      Title: Effects of divalent cations on bovine testicular hyaluronidase catalyzed transglycosylation of chondroitin sulfates.
    4. results clearly demonstrate that ejaculated bull sperm possess two forms of sperm adhesion molecule 1(SPAM1): one (epididymal) expressed at the surface, and one (testicular) that interacts with the zona pellucida after the acrosome exocytosis.
      Title: SPAM1 isoforms from two tissue origins are differentially localized within ejaculated bull sperm membranes and have different roles during fertilization.
    5. The hydrolysis Vm values strongly suggest that as the chain length decreases the hyaluronidase increasingly catalyses transglycosylation rather than hydrolysis.
      Title: Chain-length dependence of the kinetics of the hyaluronan hydrolysis catalyzed by bovine testicular hyaluronidase.
    6. The simplicity, sensitivity, and versatility of the fluorescent substrate (termed FRET-HA) suggest that it will have utility in a variety of assay platforms and should be a new tool for assessing hyaluronidase activity.
      Title: Development of a fluorescent substrate to measure hyaluronidase activity.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Clone Names

    • MGC134590

    Gene Ontology Provided by RefSeq

    Function Evidence Code Pubs
    enables hyalurononglucosaminidase activity IEA
    Inferred from Electronic Annotation
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in carbohydrate metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		 PubMed 
    involved_in fusion of sperm to egg plasma membrane involved in single fertilization IEA
    Inferred from Electronic Annotation
    more info
    		 PubMed 
    involved_in hyaluronan catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in acrosomal vesicle IEA
    Inferred from Electronic Annotation
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    sperm adhesion molecule 1
    Names
    hyaluronidase, zona pellucida binding
    NP_001008413.3
    XP_024846268.1
    XP_024846269.1
    XP_024846271.1
    XP_059741399.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001008413.3 → NP_001008413.3  sperm adhesion molecule 1 precursor

      See identical proteins and their annotated locations for NP_001008413.3

      Status: VALIDATED

      Source sequence(s)
      NKLS02000004
      UniProtKB/TrEMBL
      F1MTV1, Q2YDK3
      Related
      ENSBTAP00070040613.1, ENSBTAT00070066607.1
      Conserved Domains (1) summary
      pfam01630
      Location:42 → 371
      Glyco_hydro_56; Hyaluronidase

    RefSeqs of Annotated Genomes: GCF_002263795.3-RS_2024_12

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference ARS-UCD2.0 Primary Assembly

    Genomic

    1. NC_037331.1 Reference ARS-UCD2.0 Primary Assembly

      Range
      88202791..88222320
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_024990500.2 → XP_024846268.1  sperm adhesion molecule 1 isoform X1

      UniProtKB/TrEMBL
      F1MTV1, Q2YDK3
      Conserved Domains (1) summary
      pfam01630
      Location:42 → 371
      Glyco_hydro_56; Hyaluronidase

    2. XM_059885416.1 → XP_059741399.1  sperm adhesion molecule 1 isoform X1

      UniProtKB/TrEMBL
      F1MTV1, Q2YDK3

    3. XM_024990503.2 → XP_024846271.1  sperm adhesion molecule 1 isoform X2

      UniProtKB/TrEMBL
      Q2YDK3
      Related
      ENSBTAP00070040562.1, ENSBTAT00070066538.1
      Conserved Domains (1) summary
      pfam01630
      Location:42 → 341
      Glyco_hydro_56; Hyaluronidase

    4. XM_024990501.2 → XP_024846269.1  sperm adhesion molecule 1 isoform X1

      UniProtKB/TrEMBL
      F1MTV1, Q2YDK3
      Conserved Domains (1) summary
      pfam01630
      Location:42 → 371
      Glyco_hydro_56; Hyaluronidase
    Nucleotide Protein Strain
    Heading Accession and Version

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