Nos2 nitric oxide synthase 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Nos2provided by RGD
Full Name: nitric oxide synthase 2provided by RGD
Primary source: RGD:3185
See related: EnsemblRapid:ENSRNOG00000057443 AllianceGenome:RGD:3185
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: iNos; Nos2a
Summary: Enables several functions, including Hsp90 protein binding activity; beta-catenin binding activity; and enzyme binding activity. Involved in several processes, including cellular response to cytokine stimulus; response to bacterium; and response to hexose. Acts upstream of or within cellular response to cytokine stimulus and cellular response to organic cyclic compound. Located in several cellular components, including extracellular space; perinuclear region of cytoplasm; and peroxisome. Used to study several diseases, including Alzheimer's disease; diabetic angiopathy; pleurisy; renal hypertension; and toxic shock syndrome. Biomarker of several diseases, including acute necrotizing pancreatitis; artery disease (multiple); inflammatory bowel disease (multiple); liver disease (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including dementia (multiple); hypertension (multiple); malaria; pulmonary tuberculosis; and type 2 diabetes mellitus. Orthologous to human NOS2 (nitric oxide synthase 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Adrenal (RPKM 16.6), Heart (RPKM 10.1) and 5 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q25

Exon count:: 28

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (64313335..64349221)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (63815308..63851208)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (66188290..66221621)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Analyzing the FMN-heme interdomain docking interactions in neuronal and inducible NOS isoforms by pulsed EPR experiments and conformational distribution modeling.
Title: Analyzing the FMN-heme interdomain docking interactions in neuronal and inducible NOS isoforms by pulsed EPR experiments and conformational distribution modeling.
Upregulation of NADPH-oxidase, inducible nitric oxide synthase and apoptosis in the hippocampus following impaired insulin signaling in the rats: Development of sporadic Alzheimer's disease.
Title: Upregulation of NADPH-oxidase, inducible nitric oxide synthase and apoptosis in the hippocampus following impaired insulin signaling in the rats: Development of sporadic Alzheimer's disease.
Temporal and spatial effects on C-reactive protein's regulation of inducible nitric oxide synthase production in periodontal disease.
Title: Temporal and spatial effects on C-reactive protein's regulation of inducible nitric oxide synthase production in periodontal disease.
Inhibition of TLR4, NF-kappaB, and INOS pathways mediates ameliorative effect of syringic acid in experimental ulcerative colitis in rats.
Title: Inhibition of TLR4, NF-κB, and INOS pathways mediates ameliorative effect of syringic acid in experimental ulcerative colitis in rats.
Role of the iNOS isoform in the cardiovascular dysfunctions of male rats with 6-OHDA-induced Parkinsonism.
Title: Role of the iNOS isoform in the cardiovascular dysfunctions of male rats with 6-OHDA-induced Parkinsonism.
Hypoglycaemia aggravates impaired endothelial-dependent vasodilation in diabetes by suppressing endothelial nitric oxide synthase activity and stimulating inducible nitric oxide synthase expression.
Title: Hypoglycaemia aggravates impaired endothelial-dependent vasodilation in diabetes by suppressing endothelial nitric oxide synthase activity and stimulating inducible nitric oxide synthase expression.
Decreasing effects of protein kinase inhibitors on the expression of NOS2 and inflammatory cytokines and on phagocytosis in rat peritoneal macrophages is partly related to repolarization.
Title: Decreasing effects of protein kinase inhibitors on the expression of NOS2 and inflammatory cytokines and on phagocytosis in rat peritoneal macrophages is partly related to repolarization.
The importance of vascular epithelial growth factor (VEGF) and inducible nitric oxide synthase (INOS) in rhinitis medicamentosa pathogenesis: An experimental rat model study.
Title: The importance of vascular epithelial growth factor (VEGF) and inducible nitric oxide synthase (INOS) in rhinitis medicamentosa pathogenesis: An experimental rat model study.
iNOS is essential to maintain a protective Th1/Th2 response and the production of cytokines/chemokines against Schistosoma japonicum infection in rats.
Title: iNOS is essential to maintain a protective Th1/Th2 response and the production of cytokines/chemokines against Schistosoma japonicum infection in rats.
Enhancement of heat shock protein 70 attenuates inducible nitric oxide synthase in preeclampsia complicated with fetal growth restriction.
Title: Enhancement of heat shock protein 70 attenuates inducible nitric oxide synthase in preeclampsia complicated with fetal growth restriction.

Submit: New GeneRIF Correction See all GeneRIFs (495)

General gene information

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Markers

RH94571 (e-PCR)
- UniSTS:119819

D11Bhm163 (e-PCR), detects polymorphism
- UniSTS:141601

RH132839 (e-PCR)
- UniSTS:216122

M84373 (e-PCR)
- UniSTS:124991

GDB:437694 (e-PCR)
- UniSTS:157251

D10Mco38 (e-PCR)
- UniSTS:240217

D10Mco39 (e-PCR)
- UniSTS:240218

D10Mco40 (e-PCR)
- UniSTS:240219

D10Mco42 (e-PCR)
- UniSTS:240221

Nos2 (e-PCR), detects polymorphism
- UniSTS:532120

D10Wox24 (e-PCR)
- UniSTS:517831

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables FMN binding	IBA Inferred from Biological aspect of Ancestor more info
enables FMN binding	IEA Inferred from Electronic Annotation more info
enables FMN binding	ISO Inferred from Sequence Orthology more info
enables Hsp90 protein binding	IDA Inferred from Direct Assay more info	PubMed
enables NADP binding	IEA Inferred from Electronic Annotation more info
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables arginine binding	IEA Inferred from Electronic Annotation more info
enables arginine binding	ISO Inferred from Sequence Orthology more info
enables beta-catenin binding	IPI Inferred from Physical Interaction more info	PubMed
enables cAMP-dependent protein kinase regulator activity	IDA Inferred from Direct Assay more info	PubMed
enables cadherin binding	IPI Inferred from Physical Interaction more info	PubMed
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables flavin adenine dinucleotide binding	IBA Inferred from Biological aspect of Ancestor more info
enables flavin adenine dinucleotide binding	IEA Inferred from Electronic Annotation more info
enables flavin adenine dinucleotide binding	ISO Inferred from Sequence Orthology more info
enables heme binding	IEA Inferred from Electronic Annotation more info
enables heme binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nitric-oxide synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables nitric-oxide synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables nitric-oxide synthase activity	IEA Inferred from Electronic Annotation more info
enables nitric-oxide synthase activity	ISO Inferred from Sequence Orthology more info
enables nitric-oxide synthase activity	TAS Traceable Author Statement more info	PubMed
enables nitric-oxide synthase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables tetrahydrobiopterin binding	IEA Inferred from Electronic Annotation more info
enables tetrahydrobiopterin binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in Fc-gamma receptor signaling pathway involved in phagocytosis	IEA Inferred from Electronic Annotation more info
involved_in Fc-gamma receptor signaling pathway involved in phagocytosis	ISO Inferred from Sequence Orthology more info
involved_in G protein-coupled receptor signaling pathway coupled to cGMP nucleotide second messenger	IDA Inferred from Direct Assay more info	PubMed
involved_in arginine catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in arginine catabolic process	IEA Inferred from Electronic Annotation more info
involved_in arginine catabolic process	ISO Inferred from Sequence Orthology more info
involved_in blood vessel remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cGMP-mediated signaling	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to cytokine stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to interleukin-1	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to lipopolysaccharide	IEA Inferred from Electronic Annotation more info
involved_in cellular response to lipopolysaccharide	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within cellular response to lipopolysaccharide	ISO Inferred from Sequence Orthology more info
involved_in cellular response to lithium ion	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within cellular response to organic cyclic compound	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to redox state	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to tumor necrosis factor	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to type II interferon	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to type II interferon	ISO Inferred from Sequence Orthology more info
involved_in cellular response to vitamin D	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to xenobiotic stimulus	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to xenobiotic stimulus	ISO Inferred from Sequence Orthology more info
involved_in circadian rhythm	IEA Inferred from Electronic Annotation more info
involved_in circadian rhythm	ISO Inferred from Sequence Orthology more info
involved_in defense response to Gram-negative bacterium	IEP Inferred from Expression Pattern more info	PubMed
involved_in defense response to bacterium	IBA Inferred from Biological aspect of Ancestor more info
involved_in defense response to bacterium	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within defense response to bacterium	ISO Inferred from Sequence Orthology more info
involved_in defense response to bacterium	ISO Inferred from Sequence Orthology more info
involved_in defense response to bacterium	ISS Inferred from Sequence or Structural Similarity more info
involved_in endothelial cell proliferation	IEP Inferred from Expression Pattern more info	PubMed
involved_in glycoside metabolic process	IEP Inferred from Expression Pattern more info	PubMed
involved_in inflammatory response	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of blood pressure	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in nitric oxide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in nitric oxide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in nitric oxide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in nitric oxide biosynthetic process	TAS Traceable Author Statement more info	PubMed
involved_in nitric oxide mediated signal transduction	IBA Inferred from Biological aspect of Ancestor more info
NOT acts_upstream_of_or_within ovulation from ovarian follicle	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-cysteine S-nitrosylation	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-cysteine S-nitrosylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of interleukin-6 production	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of interleukin-6 production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-6 production	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of interleukin-8 production	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of interleukin-8 production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-8 production	ISS Inferred from Sequence or Structural Similarity more info
involved_in prostaglandin secretion	IEA Inferred from Electronic Annotation more info
involved_in prostaglandin secretion	ISO Inferred from Sequence Orthology more info
involved_in prostaglandin secretion	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of blood pressure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell population proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in regulation of cytokine production involved in inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in regulation of cytokine production involved in inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in regulation of cytokine production involved in inflammatory response	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of insulin secretion	IEA Inferred from Electronic Annotation more info
involved_in regulation of insulin secretion	ISO Inferred from Sequence Orthology more info
involved_in response to activity	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to bacterium	ISO Inferred from Sequence Orthology more info
involved_in response to dexamethasone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to estradiol	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to fructose	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to glucose	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to hormone	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to hormone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to hypoxia	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to hypoxia	ISO Inferred from Sequence Orthology more info
involved_in response to immobilization stress	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to lipopolysaccharide	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to lipopolysaccharide	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to lipopolysaccharide	ISO Inferred from Sequence Orthology more info
involved_in response to mechanical stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to oxygen-glucose deprivation	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to tumor necrosis factor	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retinoic acid receptor signaling pathway	IEP Inferred from Expression Pattern more info	PubMed
involved_in superoxide metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within superoxide metabolic process	ISO Inferred from Sequence Orthology more info
involved_in superoxide metabolic process	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cortical cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cortical cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisome	IEA Inferred from Electronic Annotation more info
located_in peroxisome	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: nitric oxide synthase, inducible

Names: NOS type II; inducible NO synthase; inducible NOS; inducible nitric oxide synthase; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase, inducible-like; peptidyl-cysteine S-nitrosylase NOS2

XP_038941131.1

EC 1.14.13.39

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001429940.1 → NP_001416869.1 nitric oxide synthase, inducible isoform 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000010

UniProtKB/TrEMBL

F1LSH9
NM_012611.4 → NP_036743.4 nitric oxide synthase, inducible isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000010

UniProtKB/TrEMBL

M0RDG2

Related

ENSRNOP00000067662.2, ENSRNOT00000073138.4

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

64313335..64349221

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039085203.2 → XP_038941131.1 nitric oxide synthase, inducible isoform X1

Related

ENSRNOP00000016133.5, ENSRNOT00000016133.8

Conserved Domains (3) summary

cd00795
Location:80 → 492

NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...

pfam00258
Location:537 → 668

Flavodoxin_1

cl06868
Location:732 → 1128

FNR_like; Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron transport ...